Clinical and Histopathological Evaluation of Using Platelet-Rich Plasma and Platelet-Rich Fibrin Matrix in Treatment of Induced Chronic Open Wounds in Bucks

Abstract

Objective: This study was designed to identify the effectiveness of platelet-rich plasma gel (PRP) and platelet-rich fibrin matrix (PRF) application on the induced open chronic full-thickness cutaneous wounds in goats.Methods: A total of 24 adult apparently healthy local breed bucks were used in this study. PRP gel and PRF matrix were prepared from whole blood at the time of treatment, four square full-thickness skin wounds (4 cm × 4 cm) on the dorsal sides of the back of each animal were created, by daily scratched to interrupt healing process continuation to prolong inflammatory reaction to form chronic wound, this surgical procedure was continued for 8 weeks to ensure the traditional chronicity wounds, after that they were treated by PRP gel in the first group (n=12), while PRF matrix was used in the second group (n=12). Each group was also subdivided into treatment (n=2 wounds) and control group (n=2 wounds) in each animal. The healing process was evaluated by clinical examination during experimental period and histologically in day 7, 14, 28, and 45 post operation. Conclusion:Both groups showed faster tissue repair and epithelialization in treatment groups compared to control groups. The results of this study confirmed PRP gel and PRF matrix application improved and accelerated cutaneous open chronic wound healing.

The Effect of Platelet-Rich Fibrin on Donor Site of Split Thickness Skin Graft in Burned Patients

“…In our study, mononuclear inflammatory cells were found in PRF-injected areas versus the untreated donor site which was in line with results discovered by other authors [17,18]. In addition, mature thick collagen fibers were seen well organized in the dermis at the PRF-injected donor site, in contrast to the control donor location, where immature collagen fibers were commonly visible and this was in line with the findings of other researchers [17,18,21,22].…”

Isolation, Purification, and Characterization of Bovine Tendon Collagen and Analysis of Glycine, Proline, and Hydroxyproline by High-Performance Liquid Chromatography-Fluorescence

“…The percentages were calculated according to the linear regression equation of each amino acid. The mean percentages of glycine, proline, and hydroxyproline were 33.247±0.20%; 11.867±0.20%; and 10.510±0.23%, which were similar to the levels of bovine collagen reported by Schrieber and Gareis, i.e., 33% glycine, 11% proline, and 10% hydroxyproline [9,10].…”

Histological characteristics of Platelet-Rich Fibrin clots obtained under various modes of blood centrifugation

“…Удосконалення технологій отримання речовин, концентруючих тромбоцити, сприяло розвитку другого покоління цих біоматеріалів, а саме фібрину, збагаченого тромбоцитами (PRF -Platelet-Rich Fibrin) (Dohan Ehrenfest et al, 2012;Tina Roshini et al, 2019;Chernyshenko et al, 2019). Аутологічні продукти першого та другого покоління відрізняються один від одного за фізичними властивостями, а також за способами їх одержання, в тому числі за кількістю ступенів центрифугування (Pazzini et al, 2016;Khalaf & Salih, 2018;Yaltirik et al, 2018). Плазму, збагачену тромбоцитами, виготовляють шляхом відбору зразка крові з різними антикоагулянтами та наступним одночи двоступеневим центрифугуванням, у результаті чого отримують очищену плазму, збагачену тромбоцитами, або плазму, збагачену лейкоцитами та тромбоцитами.…”