Effectiveness of platelet-rich fibrin matrix treated with silver nanoparticles in fracture healing in rabbit model

Abstract

Aim:The current study was conducted to evaluate the effect of platelet-rich fibrin matrix (PRFM) treated with silver nanoparticles (AgNPs) on enhancing the healing of the experimentally induced bone gap in a rabbit model.Materials and Methods:Twenty healthy male local rabbits aged between 6 and 8 months, their weights between 1.5 and 2 kg were used in this study and divided randomly into four equal groups, under general anesthesia (1 cm), bone gap was induced in the tibia bone to create a critical bone defect and leave it without any treatment in the first group (control group). While in the second group the bone gap was filled with PRFM; in the third group, the gap was filled with 0.3 ml AgNPs; and in the fourth group, the gap was filled with PRFM treated with AgNPs.Results:There was no infection at the operation site in all experimental animals, and the radiograph images showed periosteal and endosteal reaction; the gaps were bridged faster in the fourth group as compared with the other groups. The histological examination showed lamellar bone with haversian canal completely filled the fracture gap and contact with old bone in the fourth group as compared to other groups.Conclusion:Using a combination of PRFM and single nucleotide polymorphisms together gave better acceleration in the bone healing process than using each one of them separately.

Standardization of Animal Models and Techniques for Platelet-Rich Fibrin Production: A Narrative Review and Guideline

“…This method is convenient to perform, and it is used for collecting larger volumes of blood and is best suited for the production of PRF and other blood concentrates. The jugular vein allows a faster and more significant amount of blood than the marginal ear vein, making it less stressful for the rabbit than a heart puncture [ 87 ].…”

Flunixin Meglumine Enhanced Bone Fracture Healing in Rabbits Associated with Activation of Early Collagen Deposition and Enhancement of Vascular Endothelial Growth Factor Expression

“…Animals were randomly divided into 3 groups as follows: a control group in which animals received saline solution as a placebo for 5 successive days, an FM group in which animals were intramuscularly injected with 2 mg/kg bwt of FMne (Finadyne ® , MSD Animal Health, Walton, UK) once daily for 5 successive days, and a ketoprofen group in which animals were intramuscularly injected 2 with mg/kg bwt of ketoprofen (Ketolgin, Amoun, Al Obour, Egypt) once daily for 5 successive days [1,21,23].…”

“…Two radiographic views (ventrodorsal and lateral) were taken. The X-ray was performed before and after surgical procedures at 0, 2, 4, 6, and 8 weeks after operation, as mentioned in [1].…”

“…The bone healing process involves multi-sequential events associated with numerous cellular functions that promote mineralization of the fracture site followed by remodeling Animals 2021, 11, 2834. https://doi.org/10.3390/ani11102834 https://www.mdpi.com/journal/animals to return the affected bone to its original structure [1]. In the literature, many postoperative drugs, including antibiotics [2] and anti-inflammatory drugs [3][4][5][6], have been used in different stages of the healing process.…”

Advanced platelet-rich fibrin plus gold nanoparticles enhanced the osteogenic capacity of human mesenchymal stem cells

“…Interestingly, the study of PRF exudates on PDL cells showed increased proliferation and osteoblastic differentiation rate [18]. These data stand for a fact that the extraction of PRF could also be an alternative modality for the induction of cell differentiation [19, 20]. However, the effect of the fibrinous substrate must not be neglected in favor of stem cell differentiation toward specific lineages.…”