A Comparative Histopathological Study of Repaired Tendons Wrapped with Two Biological Matrices in Bucks

Abstract

| This study was planned to evaluate the efficacy of two biological matrices represented by autologous platelet rich fibrin matrix (PRFM), as well as a cross linked decellularized caprine pericardial extracellular matrix (PECM) on enhancing healing of the experimentally severed superficial digital flexor tendon in a goat model. It was carried out on 48 adult bucks, which were divided randomly into three equal groups. Under sedation and regional anesthesia, SDFT was severed at the mid metacarpal region of the right forelimb. In the first control group, tenorrhaphy was performed and left without additives. While in the second group the tenorrhaphy site was wrapped with autologous platelet rich fibrin strips which were prepared at the time of operation, as well as in the third group the tenorrhaphy site was wrapped with a cross linked decellularized PECM strip which was previously prepared from the whole fresh caprine pericardium. The histopathological evaluation in this study revealed that the neovascularization, cellularity and collagen fiber alignment scores were significantly increased in both treated groups as compared to the control group. In conclusion, both biological matrices led to improvement in tendon healing and repair, without any complications, but the PECM gave superior results in tendon repair when compared to other groups.

Macroscopic Effect of Small Intestine Submucosa Hydrogel-Silver Nanoparticles Composite on Healing of Infected Wounds

“…The management of wound is differing by the use of antiseptics and antimicro-bials, adherent and non-adherent dressings, and miscellaneous topical applications (Shi et al, 2020). Small intestinal submucosa (SIS) is a natural biomaterial derived from the small intestine of vertebrates; That consists of collagen, proteoglycan glycosaminoglycan, glycoprotein, and growth factors, all of which together make SIS an excellent choice as an ideally implanted material for tissue engineering and clinical applications (AL-Falahi and Salih, 2016). The benefits of using SIS are two folds; firstly, it provides a scaffold to reconstruct and repair the host tissue, and secondly, it signals the surrounding host cells to grow, to form new vessels, and to foster cellular differentiation, which initiates site specific tissue remodeling Wang et al, (2018).…”

Ultra-Sonographic Evaluation and Growth Factors Measurement of Using Tendon Derived Hydrogel and Hyaluronic Acid in Tendon Repair in Rams

“…The emerging field of Tissue Engineering and Regenerative Medicine is increasingly being employed to design strategies for the repair of tendon tissue. These strategies are focused on the activation and enhancement of the body's own repair system by using a combinatory approach that may include the application of cells, stimulatory factors, genes, and scaffolds (2).The published researches describe the production of decellularized scaffolds from non-homologous anatomical sites such as using of small intestine submucosa (3)(4), Bovine Urinary Bladder Sub-mucosa (5), and pericardium and platelet-rich fibrin matrix (PRFM) (6). In the last decade researches had also shown the efficacy of extracellular matrix hydrogels derived from the tissue targeted for treatment of tendon injuries (7)(8).…”