SummaryThe results and agreements of the 1st international BoLA workshop, held in Edinburgh, Scotland in August 1978, are reported. Most of these concern the results from a comparison test of 249 alloantisera to bovine lymphocytes, the antisera being contributed by 9 laboratories. These sera were compared directly in Edinburgh on a panel of lymphocytes from 130 cattle of 21 breeds. In the micro‐lymphocytotoxicity test used 75% of the sera reacted. Sixty eight of these sera were grouped into clusters according to their reaction patterns against the lymphocyte panel. Eleven of these clusters were clearly defined and were given workshop BoLA designations. In addition 22 sera were assigned to subgroups of the agreed clusters. There was no evidence that the method of production of the sera had any effect on their specificity.Although genetic data was not available, the phenotypes of the test panel of lymphocytes are consistent with the clusters detecting antigens controlled by multiple alleles at a single autosomal locus. It was agreed to name the genetic region where this putative locus is located BoLA (bovine lymphocyte antigen).
Two hundred and eighty-two alloantisera were submitted by 20 participating laboratories from 13 countries and tested against lymphocytes of 1298 cattle. The cell panel consisted of samples from 38 Bos tuurus breeds, 11 Bos tuurus crossbreeds, 4 Bos indicus breeds, 6 Bos tuurus X Bos indicus, and a variety of other crossbred populations. Using a standardized lymphocytotoxicity test, all 17 previously identified BoLA specificities were confirmed. The workshop produced agreement on 16 new lymphocyte alloantigenic specificities. Three of the new specificities behaved as splits of previously identified BoLA specificities. Four of the new specificities behaved as alleles at the agreed BoLA-A locus. Seven new specificities are tentatively assigned to the BoLA-A locus but require further definition. Two new specificities may represent products of a second closely-linked BoLA locus.
Zusammenfassung Die von uns gewonnenen Isoimmunseren von Schafen enthielten gegen Lymphozyten gerichtete zytotoxische Antikörper. Mit diesen Immunseren wurden an Lymphozyten von Schafen 31 verschiedene Lymphozytenantigene nachgewiesen und die Phänotypfrequenzen beim Merinolandschaf ermittelt. Genetisch determinierte R‐Rezeptoren wurden sowohl auf den Lymphozyten als auch auf den Erythrozyten aller R‐positiven Schafe nachgewiesen. Summary Lymphocyte antigens in the sheep Immune sera containing cytotoxic anti‐lymphocyte antibodies were obtained through iso‐immunization of sheep. Using these sera, 31 different antigens were detected on lymphocytes of the German Merino Landrace, and the phenotype‐frequencies for this breed were established. Genetically determined R‐receptors were found to be present on both lymphocytes and erythrocytes of all R‐positive sheep. Résumé A propos des antigènes lymphocytaires chez le mouton Des isoimmunosérums de moutons contenant des anticorps cytotoxiques antilymphocytaires ont été obtenus. 31 antigènes lymphocytaires différents furent mis en évidence sur des lymphocytes de moutons à partir de ces immunosérums et les fréquences des phénotypes ont été trouvées chez le mouton «Merinos». Les récepteurs R génétiquement déterminants ont été situés aussi bien sur les lymphocytes que sur les érythrocytes de tous les moutons R‐positifs. Resumen Sobre los antígenos linfocitarios en la oveja Los sueros isoinmunes de ovejas, obtenidos por nosotros, contenían anticuerpos citotóxicos dirigidos contra los linfocitos. Con estos sueros inmunes se identificaron en linfocitos de ovejas 31 antígenos linfocitarios diferentes, y se determinaron las frecuencias fenotípicas en la oveja merina rural. Se identificaron receptores R, determinados genéticamente, tanto en los linfocitos como en los eritrocitos de todas las ovejas R‐positivas.
Blood samples from 54 animals were exchanged between 15 laboratories in nine countries to improve and expand BoLA class 1 and class I1 typing. A total of 27 out of 33 (82%) of previously accepted BOLA-w specificities were represented within the cell panel. Seventeen new serum-defined BoLA specificities were accepted by the workshop participants, thus expanding the number of internationally recognized BoLA specificities to 50. The large number of new specificities detected resulted from the number of serological reagents used ( n = 1139) and the genetic diversity of the cell panel. Confidence derived from the high percentage of agreement between the laboratories on antigen detection (97.3%; r = 0.84) permitted the removal of the workshop (w) notation from 23 BOLA-w specificities and their acceptance as full status BOLA-A antigens. Two new non-BOLA antigens were also detected, one completely included within the red blood cell factor S' (BoLy-S'), whereas a second (BoLy-wl) did not show any association with tested red blood cell factors. A comparison between serological, isoelectric focusing (IEF) and DNA typing for BoLA class I1 polymorphism was conducted with a subset of workshop cells. Correlation between the three methods was significant for three combinations of alleles. Three other serologically defined class I1 specificities were correlated with DR and/or DQ restriction fragment length polymorphism (RFLP) types, whereas six additional IEF types were correlated with DR and/or DQ RFLP types ( r L 0.50). Several new IEF, DRB, DQA and DQB RFLP patterns were identifed. In 46 animals that were typed for BOLA-DR and DQ genes by RFLP analysis, 46 different BoLA haplotypes were tentatively defined. These 46 haplotypes were distinguished by 31 serologically-defined BOLA-A alleles (and 2 'blanks'), 15 DRB RFLP types (plus up to 10 new DRB RFLP patterns) and 23 DQA-DQB haplotypes.
Summary One hundred and twelve pig sera were screened for their inhibitory properties to cytolytic reactions of eighteen reagents obtained after skin grafting. All of the tested pig sera discriminately inhibited the cytotoxicity of various reagents, which clearly demonstrated that the inhibitions are of defined specificities. This could be confirmed in pig families. In a number of instances serum contained inhibitors of such specificities that were not detectable on the lymphocyte membrane of the animal the serum was taken from. The aim of this study was not to identify the specificities involved. Nevertheless the SL‐A3 specificity was observed in serum of eighty‐four out of 112 animals, whereby the SL‐A3 antigen was detectable on the lymphocyte membrane of only fourteen of those animals. The occurrence of histocompatibility inhibitors in serum of pigs is of importance in experimental organ grafting and in immunizations of animals when reagents of certain specificities are attempted to be produced. Granulocytes appear also to be furnished with cell bound histocompatibility antigens.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.