Joint Report of the Third International Bovine Lymphocyte Antigen (BoLA) Workshop, Helsinki, Finland, 27 July 1986

Abstract

Two hundred and eighty-two alloantisera were submitted by 20 participating laboratories from 13 countries and tested against lymphocytes of 1298 cattle. The cell panel consisted of samples from 38 Bos tuurus breeds, 11 Bos tuurus crossbreeds, 4 Bos indicus breeds, 6 Bos tuurus X Bos indicus, and a variety of other crossbred populations. Using a standardized lymphocytotoxicity test, all 17 previously identified BoLA specificities were confirmed. The workshop produced agreement on 16 new lymphocyte alloantigenic specificities. Three of the new specificities behaved as splits of previously identified BoLA specificities. Four of the new specificities behaved as alleles at the agreed BoLA-A locus. Seven new specificities are tentatively assigned to the BoLA-A locus but require further definition. Two new specificities may represent products of a second closely-linked BoLA locus.

One‐dimensional isoelectric focusing and immunoblotting of bovine major histocompatibility complex (BoLA) class I molecules and correlation with class I serology

“…The BoLA class I typing was performed on peripheral blood lymphocytes, using a panel of 70 BoLA alloimmune antisera in a microlymphocytotoxic test according to the standards of the Third International BoLA Comparison Test (Bull et al 1989). The antisera recognized the following 27 specificities behaving as alleles of the w18(w6), w19(w6), w20, w21, w22, w23(w5), w26, w28, w31(w30), cph22(w28), cph43(w30), cph45(w9), cph48, cph49, cph63(w28), where w indicates an internationally defined specificity and parenthesis indicates the super-typic specificity for splits.…”

“…Genetic variation at the bovine major histocompatibility complex (BoLA) class I loci has been studied by serological, biochemical and DNA methods. In the Third BoLA Comparison Test, 33 serologically defined BoLA class I specificities were described of which all but two were assigned to a single class I locus, designated BOLA-A (Bull et al 1989). The biochemical studies, employing isoelectric focusing (IEF), have used immunoprecipitation with the monoclonal antibodies w6/32 and B1.1G6 (Joosten et al 1988;Oliver et al 1989;Watkins et al 1989).…”

Biochemical evidence of the expression of two major histocompatibility complex class I genes on bovine peripheral blood mononuclear cells

“…The polymorphism of class I antigens of the bovine major histocompatibility complex (MHC) is defined by serological (Bull et al 1989) and biochemical methods (Joosten et al 1988;Lindberg & Andersson 1988;Oliver et al 1989;Watkins et al 1989). Class I bovine lymphocyte antigen (BoLA) products are 44-45kD glycoprotein molecules that are non-covalently associated with the 12-kD P2-microglobulin (Huang-Xuan et al 1982;Bensaid et al 1988).…”

“…Class I bovine lymphocyte antigen (BoLA) products are 44-45kD glycoprotein molecules that are non-covalently associated with the 12-kD P2-microglobulin (Huang-Xuan et al 1982;Bensaid et al 1988). The Third International BoLA Workshop (Bull et al 1989) has established more than 30 serologically defined specificities which appear to be encoded at a single class I locus. There is limited evidence for the fact that some serological reagents detect products of a second locus (Stear ef al.…”

Identification and characterization of new BoLA‐DRB3 alleles by heteroduplex analysis and direct sequencing

“…The gene complex is divided into two regions, BoLA-A and BOLA-D, coding for class I and class I1 antigens respectively. The BOLA-A region has been defined serologically, and 31 internationally accepted specificities have been recognized (Bull et al 1989;Bernoco eta]. 1991).…”