Joint Report of the Fourth International Bovine Lymphocyte Antigen (BoLA) Workshop, East Lansing, Michigan, USA, 25 August 1990

Abstract

Blood samples from 54 animals were exchanged between 15 laboratories in nine countries to improve and expand BoLA class 1 and class I1 typing. A total of 27 out of 33 (82%) of previously accepted BOLA-w specificities were represented within the cell panel. Seventeen new serum-defined BoLA specificities were accepted by the workshop participants, thus expanding the number of internationally recognized BoLA specificities to 50. The large number of new specificities detected resulted from the number of serological reagents used ( n = 1139) and the genetic diversity of the cell panel. Confidence derived from the high percentage of agreement between the laboratories on antigen detection (97.3%; r = 0.84) permitted the removal of the workshop (w) notation from 23 BOLA-w specificities and their acceptance as full status BOLA-A antigens. Two new non-BOLA antigens were also detected, one completely included within the red blood cell factor S' (BoLy-S'), whereas a second (BoLy-wl) did not show any association with tested red blood cell factors. A comparison between serological, isoelectric focusing (IEF) and DNA typing for BoLA class I1 polymorphism was conducted with a subset of workshop cells. Correlation between the three methods was significant for three combinations of alleles. Three other serologically defined class I1 specificities were correlated with DR and/or DQ restriction fragment length polymorphism (RFLP) types, whereas six additional IEF types were correlated with DR and/or DQ RFLP types ( r L 0.50). Several new IEF, DRB, DQA and DQB RFLP patterns were identifed. In 46 animals that were typed for BOLA-DR and DQ genes by RFLP analysis, 46 different BoLA haplotypes were tentatively defined. These 46 haplotypes were distinguished by 31 serologically-defined BOLA-A alleles (and 2 'blanks'), 15 DRB RFLP types (plus up to 10 new DRB RFLP patterns) and 23 DQA-DQB haplotypes.

Detección de los polimorfismos de 4 STRS en la región Bola y análisis de su efecto sobre Mastitis clínica Bovina en la raza Holstein

“…La región clase I designada como BoLA A, ha sido definida serológicamente por Amorena y Stone (1978) y comprende 50 aloantígenos reconocidos internacionalmente (Bernoco et al, 1991). Los genes BoLA clase II están distribuidos en dos regiones diferentes IIA y IIB (Davies et al, 1994).…”