Immunogenetic Properties of Sl‐a Inhibitor Substances in Serum of Pigs*

Abstract

Summary One hundred and twelve pig sera were screened for their inhibitory properties to cytolytic reactions of eighteen reagents obtained after skin grafting. All of the tested pig sera discriminately inhibited the cytotoxicity of various reagents, which clearly demonstrated that the inhibitions are of defined specificities. This could be confirmed in pig families. In a number of instances serum contained inhibitors of such specificities that were not detectable on the lymphocyte membrane of the animal the serum was taken from. The aim of this study was not to identify the specificities involved. Nevertheless the SL‐A3 specificity was observed in serum of eighty‐four out of 112 animals, whereby the SL‐A3 antigen was detectable on the lymphocyte membrane of only fourteen of those animals. The occurrence of histocompatibility inhibitors in serum of pigs is of importance in experimental organ grafting and in immunizations of animals when reagents of certain specificities are attempted to be produced. Granulocytes appear also to be furnished with cell bound histocompatibility antigens.

Serologically defined specificities of the pig main histocompatibility complex (SL‐A)

“…They also confirmed the association between classic, serologically determinable products (SD products) and the degree of blastic transformation of allogenic lymphocytes in a mixed culture (MLC) for which lymphocyte-defined (LD) products are responsible (Vaiman et al, 1970b;White et al, 1973;Sachs et al, 1976). In addition, recombination between the genes controlling SD and LD products was described (Vaiman et al, 1973), SL-A linkage to the J blood group locus was detected (Hruban et al,19'761, serum specific SL-A inhibitors were found (Cwik & Schmid, 1975) and the existence of B lymphocytic alloantigens controlled from a region responsible for blastic transformation in a MLC was described (Vaiman et al, 1975).…”