Early diagnosis and treatment of meningitis are very important for reducing its mortality and morbidity. In patients with suspected meningitis, early detection of the responsible agents may be possible with molecular methods, such as PCR. Significant economic benefits may be obtained by preventing unnecessary antibiotic use and hospitalizations through the early detection of the microbial agents.
ÖZET SUMMARY Evaluation of Antimicrobial Susceptibility of Staphylococcus aureus Strains Isolated from Blood Cultures in Konya Training and Research Hospital Between the Years 2009-2013 Bloodstream infections have high morbidity and mortality rates. Staphylococcus aureus is one of the most common causes of bloodstream infections. Evaluation of frequency of microorganisms isolated from blood cultures and antibiotic susceptibility can provide clinicians with relevant infomation for the emprical treatment of patients. In this study, antimicrobial susceptibility patterns of S.aureus strains isolated from bloodstream infections have been evaluated. Blood cultures were performed with automated system; BACTEC 9240 (Becton Dickinson, USA). Phoenix-100 (Becton Dickinson-USA) automated system were used for identification and antimicrobial susceptibility. Susceptibility was determined using the CLSI interpretive criteria.During a period of five years, a total of 409 isolates of S.aureus were evaluated and 33 % were found methicillinresistant. The susceptibility rates of trimethoprim-sulfamethoxazole, gentamycin, erythromycin, tetracycline, clindamycin and rifampin in methicillin-resistant strains were 74.3 %, 36 %, 16.6 %, 41 %, 37.2 %, 48.6 % respectively. The susceptibility rates of trimethoprim-sulfamethoxazole, gentamycin, erythromycin, tetracycline, clindamycin and rifampin in methicillinsuscebtible strains were 89 %, 73.8 %, 59.7 %, 74 %, 66 %, 76.6 % respectively. The uncontrolled use of antibiotics increase the development of antimicrobial resistance. The creation of rational antibiotic use awareness and periodically evaluation of their own resistance profiles for each center and share with related committees would be the preventive factors against resistance development.
Objective: Acute respiratory infections are one of the most common infectious diseases. Clinical course of acute respiratory infections in young children and the elderly may be serious and life-threatening. It has been emphasized that 20-60% of etiologic agents are viruses. Respiratory syncytial virus (RSV) is the most common viral pathogen in children. On the other hand, influenza virus is the most commonly identified agent in adults. The aim of this study is to identify the viral pathogens in hospitalized patients with respiratory tract infection by using multiplex PCR method.Methods: Nasopharyngeal swab samples obtained from hospitalized patients with acute respiratory infections were enrolled between January 2013 and December 2015. The identification of influenza virus type A and B, human rhinovirus (HRV), respiratory syncytial virus A and B (RSV A-B), parainfluenza virus type 1, 2, 3, and 4, adenovirus, bocavirus, human coronavirus, human enterovirus and human metapneumovirus (HMPV) in nasopharyngeal samples was investigated by using CLART ® Pneumovir kit based on clinical array technology (Genomica, Spain).Results: Of the 1290 patients included, 1110 (86%) were children and 180 (14%) were adults. The number of samples in which only one virus was identified was 600. In additional 150 specimens, co-infections of multiple viruses were detected. The total of positive samples was 750 (58%). The majority of these positive specimens were children's samples (694 versus 56). RSV was the most common viral agent (35%) followed by HRV (13%), and influenza (10%). The rate of co-existence of viral pathogens was 20%. The multiplex pcr results were shown in Table 1.Conclusion: While RSV was the most common viral pathogen detected inrespiratory infections, other emerging agents, such as human metapneumovirus, bocavirus, and HRV were detected in considerably high rates, suggesting these emerging agents should not be underestimated in the etiology of respiratory infections. In conclusion, in this study, it was shown that microarray-based multiplex PCR method is an easy, rapid, and sensitive diagnostic tool for diagnosis of viral respiratory infections and that utility of this method makes it essential among routine diagnostic tools in clinical microbiology laboratories. http://dx. Abstract no: 233 Presentation at ESCV 2016: Poster 193
Aim: The aim of this study was to investigate the associations between the serum levels of interleukin (IL)-6, IL-10, tumor necrosis factor (TNF)-α, IL-6/IL-10 ratio and Glasgow Coma Scale (GCS), Acute Physiology and Chronic Health Evaluation (APACHE-II), Sequential Organ Failure Assessment (SOFA) scores and mortality in patients with sepsis. Materials and Methods: The sample of the study consisted of 50 patients (median age: 75 years) presented to the Intensive Care Unit of the Emergency Department between January 2019 and December 2019 with sepsis. Blood samples were taken from all patients on day 1 and day 3. The IL-6, IL-10, TNF-α levels, APACHE-II, SOFA, and GCS scores were recorded on a data collection form. Results: The deceased and the survived groups significantly differed in day-1 (p = 0.013) and day-3 IL-6 (p = 0.016) levels, day-1 IL-6/IL-10 ratio (p = 0.029) and gender. On the other hand, there was no significant difference between the groups in day-1 and day-3 IL-10 levels and day-3 IL-6/IL-10 ratio. The GCS score was significantly lower in the deceased group than in the survived group (p < 0.05). Conclusion: High IL-6 levels and high IL-6/IL-10 ratios on the day of diagnosis with sepsis were found to be correlated with mortality. IL-6 level may be particularly useful for predicting mortality if used in combination with scoring systems such as the GCS and different clinical parameters.
Respiratory tract infections (RTI) are among the most commonly seen infections in children across the world, with the highest rate of morbidity. Viruses are known to be the primary agent in these infections and mostly lead to upper RTIs. The most commonly seen viral agents of RTIs may be listed as rhinoviruses (HRV), influenza, parainfluenza (PIV), respiratory syncytial virus (RSV), adenovirus, and enteroviruses. In this study, prediagnosed children with acute RTI between January 2013 and May 2015 were included. Nasopharyngeal swab samples obtained from a total of 2,268 patients admitted to the hospital of Meram Medical School of Necmettin Erbakan University and Ministry of Health Konya Training and Research Hospital were investigated. The nasopharyngeal swab samples obtained in the hospital of Meram Medical School were investigated via Seeplex RV12 ACE Detection multiplex PCR (Seegene, South Korea) while the samples in Konya Training and Research Hospital were assessed using the system by CLART PneumoVir (Genomica, Spain). Of the 2,268 samples investigated in the study, 1,320 (58.2%) were detected for viruses. Positive number of viruses found in both kits were collected for use in a table, and the most common viruses rates were calculated according to these numbers. Among positive 1,221 samples, 27.9% were found to be RSV B, and this rate was, in turn, followed by HRV (18.8%) and RSV A. The most frequent concomitant of double viral agents was observed in RSV B and HRV as 14% followed by RSV A and HRV as 6.8%, and PIV 3 and HRV with 5.9%. While RSV B and RSV A were encountered most in winter and spring months, HRV was determined as an infectious agent in all seasons. Multiplex polymerase chain reaction is beneficial for physicians to diagnose such viruses at an early stage. By the early detection of respiratory viruses leading to seasonal epidemics, physicians' approach to patients will become easier, and unnecessary use of antibiotics will be prevented. Additionally, our study findings are intriguing in shedding light on studies related to the development and application of vaccines.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.