Girifl ve Amaç: Bu çal›flman›n amac›, bölgemizde, hepatit C virüsü infeksiyonunun olas› bulafl›m yollar›n›n ve yak›n dönemde hepatit C virüsü genotiplerinin da¤›l›m›n›n belirlenmesidir. Gereç ve Yöntem: Hepatit C virüsü risklerinin belirlenmesi amac› ile Ege Üniversitesi Hastanesi T›bbi Mikrobiyoloji Laboratuvar›na, 2005-2010
Objective: In our study, we aimed to investigate the prevalence of rotavirus and enteric adenovirus in stool samples sent to our laboratory for antigen detection of children between 0-18 years of age admitted to hospital with diarrhea, abdominal pain, vomiting and fever and diagnosed as gastroenteritis. We also analyzed their frequencies according to demographic parameters. Methods: Results of 5156 pediatric patients admitted to Necmettin Erbakan University Meram Faculty of Medicine Hospital and diagnosed as gastroenteritis between January 2013-December 2015 were investigated retrospectively. VIKIA ® RotaAdeno (bioMérieux, Marcy l'Etoile, France), a chromatographic immunoassay detecting both viruses simultaneously was used according to the manufacturer's recommendations in stool samples. Results: Viral antigens were detected in 884 (17.1%) of the total 5156 samples. 764 (14.8%) of the positive results were detected as rotavirus and 120 (2.3%) were detected as adenovirus. Of the patients with positive results, 412 (46.6%) were female and 472 (53.4%) were male. When results are considered according to age, 2-4 age group was found to have the most common positivity (n=372) as 42.1%. Seasonal distribution of acute gastroenteritis cases was analyzed and the number of cases due to rotavirus was found to be increased in winter and spring and enteric adenoviruses were detected all year round. Conclusions: Rotavirus is the most common reason of gastroenteritis in the newborn and children, which must be considered for patients with diarrhea especially in the first four years of life. Rapid diagnosis is important for prediction of clinical implications and treatment. As enteric adenovirus is an important reason of gastroenteritis in infancy and childhood, it is necessary to investigate adenovirus antigens as well. Conducting regional studies are important for contributing to epidemiological data. Klimik Dergisi 2016; 29(3): 121-4.
ÖZObjective: In this study, it is aimed to determine the mutations responsible for drug resistance in patients with chronic hepatitis B virus (HBV) infection received/receiving antiviral treatment at our hospital and to examine the patients in terms of the treatment applied and their HBV-DNA levels. Materials and Methods:One hundred and thirty-one samples taken from patients diagnosed with chronic hepatitis B infection between January 2010 and January 2015 at Necmettin Erbakan University Meram Faculty of Medicine Hospital were studied with reverse hybridization principle-based INNO-LiPA HBV DR v2 method and the results were evaluated retrospectively. Results: Mutation was determined in 12 samples (9.1%). While tyrosine, methionine, aspartate, aspartate (YMDD) pattern change causing lamuvidine resistance was determined in 10 samples, 7 of them were observed to be M204I tyrosine, isoleucine, aspartate, aspartate (YIDD) and 4 were M204V tyrosine, valine, aspartate, aspartate (YVDD). Multiple mutations were determined in six samples (M204V+M204I+L180I, YVDD+L180M+V/G173L, YIDD+L180M, YIDD+L80V in one each and YIDD+L80I, YVDD+L180M in two each) and single mutation was determined in 3 samples (YIDD in two samples and N236T and L80V in one each). Control HBV-DNA levels were evaluated in patients with resistance gene after 6-12 months and a decrease in DNA level was observed in 11 of 12 patients. Conclusion:Since a limited number of mutations can be examined via LiPA method, it is concluded that different mutation patterns causing drug resistance cannot be determined and it will be beneficial to use an additional method such as sequencing that enables to determine these genes. Additionally, as a result of treatment failure due to drug resistance, if the treatment will be continued with a novel drug that is not used before, it is considered that the possibility of the presence of mutations causing a resistance against this antiviral should not be neglected. Keywords: Hepatitis B, drug resistance, mutationAmaç: Bu çalışmada, hastanemizde antiviral terapi almış/alan kronik hepatit B virüs (HBV) enfeksiyonu olan hastalarda ilaç direncinden sorumlu mutasyonların belirlenmesi, hastaların verilen tedavi ve HBV-DNA düzeyleri yönünden incelenmesi amaçlandı. Gereç ve Yöntemler: Necmettin Erbakan Üniversitesi Meram TıpFakültesi Hastanesi'nde Ocak 2010 -Ocak 2015 tarihleri arasında kronik hepatit B enfeksiyonu tanısı ile takip edilen hastalardan alınan ve hastanemiz mikrobiyoloji laboratuvarına gönderilen 131 örnek ters hibridizasyon temeline dayalı INNO-LiPA HBV DR v2 yöntemi ile çalışıldı ve sonuçlar retrospektif olarak incelendi. Bulgular: Yüz otuz bir örneğin 12'sinde (%9,1) mutasyon saptanmıştır. On örnekte lamuvidin direncine neden olan tirozin, metiyonin, aspartat, aspartat (YMDD) motif değişikliği belirlenirken bunların 7'si M204I tirozin, izolösin, aspartat, aspartat (YIDD), 4'ü M204V tirozin, valin, aspartat, aspartat (YVDD) şeklinde izlenmiştir. Altı örnekte çoklu mutasyon (birer örnekte M204V+M204I+L180I, YVDD+...
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