Comparison of Conventional and Molecular Methods Used for Diagnosis of Mycobacterium Tuberculosis in Clinical Samples

Akkaya, Oya; Kurtoğlu, Muhammet Güzel

doi:10.7754/clin.lab.2019.190145

Cited by 5 publications

(2 citation statements)

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“…qPCR can be used for accurate detection of mycobacteria with partial quantification, as verified by several studies. These studies focused on the detection of NTM in multiple water samples [ 20 , 25 , 26 , 27 , 28 , 29 , 30 ], as well as the detection of obligate and potentially pathogenic mycobacteria in clinical samples from humans [ 31 ], tissue samples from animals [ 32 , 33 , 34 , 35 , 36 ], and in food and feed samples [ 33 , 37 , 38 , 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Recovery of Mycobacteria from Heavily Contaminated Environmental Matrices

et al. 2021

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For epidemiology studies, a decontamination method using a solution containing 4.0% NaOH and 0.5% tetradecyltrimethylammonium bromide (TDAB) represents a relatively simple and universal procedure for processing heavily microbially contaminated matrices together with increase of mycobacteria yield and elimination of gross contamination. A contamination rate only averaging 7.3% (2.4% in Cluster S; 6.9%% in Cluster R and 12.6%% in Cluster E) was found in 787 examined environmental samples. Mycobacteria were cultured from 28.5% of 274 soil and water sediments samples (Cluster S), 60.2% of 251 samples of raw and processed peat and other horticultural substrates (Cluster R), and 29.4% of 262 faecal samples along with other samples of animal origin (Cluster E). A total of 38 species of slow and rapidly growing mycobacteria were isolated. M. avium ssp. hominissuis, M. fortuitum, and M. malmoense were the species most often isolated. The parameters for the quantitative detection of mycobacteria by PCR can be significantly refined by treating the sample suspension before DNA isolation with PMA (propidium monoazide) solution. This effectively eliminates DNA residue from both dead mycobacterial cells and potentially interfering DNA segments present from other microbial flora. In terms of human exposure risk assessment, the potential exposure to live non-tuberculous mycobacteria can be more accurately determined.

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Section: Introductionmentioning

confidence: 99%

Recovery of Mycobacteria from Heavily Contaminated Environmental Matrices

et al. 2021

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“…Rapid and precise diagnosis of active TB largely represents an unmet clinical need ( Pai and Schito, 2015 ). Traditional diagnosis has defects, such as the low sensitivity (12–15%) of acid-fast bacilli (AFB) and time-consuming nature of cultures ( Akkaya and Kurtoglu, 2019 ). Molecular diagnostics such as Xpert MTB/RIF (Cepheid, Sunnyvale, CA, United States) can achieve a sensitivity of 34–66.7% for smear negative- Pulmonary TB (PTB) and extrapulmonary TB ( Qureshi et al, 2019 ; Wu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

The Evaluation and Validation of Blood-Derived Novel Biomarkers for Precise and Rapid Diagnosis of Tuberculosis in Areas With High-TB Burden

Gong

Xiong

et al. 2021

Front. Microbiol.

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Tuberculosis (TB) remains a highly contagious public health threat. Precise and prompt diagnosis and monitoring of treatment responses are urgently needed for clinics. To pursue novel and satisfied host blood-derived biomarkers, we streamlined a bioinformatic pipeline by integrating differentially expressed genes, a gene co-expression network, and short time-series analysis to mine the published transcriptomes derived from whole blood of TB patients in the GEO database, followed by validating the diagnostic performance of biomarkers in both independent datasets and blood samples of Chinese patients using quantitative real-time PCR (qRT-PCR). We found that four genes, namely UBE2L6 (Ubiquitin/ISG15-conjugating enzyme E2 L6), BATF2 (Basic leucine zipper transcriptional factor ATF-like), SERPING1 (Plasma protease C1 inhibitor), and VAMP5 (Vesicle-associated membrane protein 5), had high diagnostic value for active TB. The transcription levels of these four gene combinations can reach up to 88% sensitivity and 78% specificity (average) for the diagnosis of active TB; the highest sensitivity can achieve 100% by parallel of BATF2 and VAMP5, and the highest specificity can reach 89.5% through a combination of SERPIG1, UBE2L6, and VAMP5, which were significantly higher than 75.3% sensitivity and 69.1% specificity by T-SPOT.TB in the same patients. Quite unexpectedly, the gene set can assess the efficacy of anti-TB response and differentiate active TB from Latent TB infection. The data demonstrated these four biomarkers might have great potency and advantage over IGRAs in the diagnosis of TB.

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A pH-responsive magnetic resonance tuning probe for precise imaging of bacterial infection in vivo

Liu

Deng

et al. 2023

Acta Biomaterialia

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Comparison of Conventional and Molecular Methods Used for Diagnosis of Mycobacterium Tuberculosis in Clinical Samples

Cited by 5 publications

References 0 publications

Recovery of Mycobacteria from Heavily Contaminated Environmental Matrices

Recovery of Mycobacteria from Heavily Contaminated Environmental Matrices

The Evaluation and Validation of Blood-Derived Novel Biomarkers for Precise and Rapid Diagnosis of Tuberculosis in Areas With High-TB Burden

A pH-responsive magnetic resonance tuning probe for precise imaging of bacterial infection in vivo

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