Background: This experiment is conducted to evaluate the anti-inflammatory effect of Piper chaba roots. Methods: The in-vitro anti-inflammatory activity of Piper chaba was carried out by human red blood cell (HRBC) membrane stabilization method which includes heat-induced hemolysis and hypo tonicity-induced hemolysis and also by another method of egg albumin denaturation assay. Results: Anti-inflammatory activity study of crude ethanolic extract was performed using heat induced membrane stabilization method, hypo-tonicity induced HRBC membrane stabilization method and egg albumin denaturation method. Crude ethanolic extracts of P. chaba showed promising in vitro anti-inflammatory activity in a concentration dependent manner. Using acetyl salicylic acid (ASA) as standard drug and was compared with ethanolic extract to determine anti-inflammatory activity. Heat induced anti-inflammatory test revealed that crude ethanolic extract of P. chaba (500 μg/ml) and positive control ASA(500 μg/ml) have 52.667% and 78% respectively, hypo tonicity induced antiinflammatory test showed 35.67% and 59% inhibition of red blood cell (RBC) hemolysis. Egg albumin denaturation method also evaluated that crude ethanolic extract (1000 μg/ml) and ASA (1000 μg/ml) showed 60% and 97.12% inhibition of egg albumin denaturation. Conclusion: The plant of P. chaba of the genus Piper possesses promising anti-inflammatory activities.

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At the end of 2019, a novel coronavirus (CoV) was found at the seafood market of Hubei province in Wuhan, China, and this virus was officially named coronavirus diseases 2019 (COVID-19) by World Health Organization (WHO). COVID-19 is mainly characterized by severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) and creates public health concerns as well as significant threats to the economy around the world. Unfortunately, the pathogenesis of COVID-19 is unclear and there is no effective treatment of this newly life-threatening and devastating virus. Therefore, it is crucial to search for alternative methods that alleviate or inhibit the spread of COVID-19. In this review, we try to find out the etiology, epidemiology, symptoms as well as transmissions of this novel virus. We also summarize therapeutic interventions and suggest antiviral treatments, immune-enhancing candidates, general supplements, and CoV specific treatments that control replication and reproduction of SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV).

BackgroundInspite of introduction of oral hypoglycemic agents, diabetes and its related complications remains to be a major clinical problem. The aim of this study was to investigate the antidiabetic, antihyperlipidemic and antioxidant activities of Grewia asiatica (Linn) stem bark in alloxan induced diabetic rats.MethodsDiabetes was induced by a single dose of intraperitoneal injection of alloxan (110 mg/kg) in Norwegian Long Evans rats. Ethanol extract of barks from Grewia asiatica (GAE 200 and 400 mg/kg) and metformin (150 mg/kg) were orally administered once daily for 15 days. Blood glucose levels and body weights of rats were measured on 0, 5, 10 and 15 days of oral treatment. At the end of the experiment the rats were sacrificed and blood sample were collected for the measurement of total cholesterol (TC), triglycerides (TG), very low density lipoproteins (VLDL), low density lipoproteins (LDL), high density lipoproteins (HDL), SGOT and CK-MB. Analysis of liver glycogen content and histopathlogy of pancreas were carried out. In vitro DPPH free radical scavenging activity, total phenolic and total flavonoid content of GAE were also determined.ResultsAfter 15 days of oral administration of GAE at doses of 200 and 400 mg/kg increased survival rate and showed a significant attenuation in blood glucose and lipid profile in diabetic rats. Oral ingestion of GAE significantly reduced the SGOT and CK-MB levels and restored liver glycogen content when compared to diabetic control. The effects of GAE on SGOT, CK-MB and liver glycogen content were dose-dependent. The diabetic rats treated with GAE showed significant improvement in normal cellular population size of islets. Phytochemical screening of GAE revealed the presence of flavonoid, steroid, tannin and phenolic compounds. Total phenolic content was 44.65 ± 3.17 mg of gallic acid equivalent per gm of GAE extract and the total flavonoid content was 39.11 ± 4.65 mg of quercetin equivalent per gm of GAE extract. In DPPH scavenging assay, IC50 values of GAE and ascorbic acid were found 76.45 and 12.50 μg/ml, respectively.ConclusionWe demonstrated that ethanol extract of barks from G. asiatica possess glucose, lipid lowering efficacy, restored liver glycogen and protects pancreas from oxidative damage in alloxan-induced diabetic rats. Thus, the results of the present study provide a scientific rationale for the use of G. asiatica in the management of diabetes and its related complications.

The antidiabetic effects of Ethyl acetate (Et-Ac), Petroleum-ether (Pet-ether), and Chloroform fractions from ethanolic extract of the leaves of Ocimum sanctum were investigated in normal and alloxan induced diabetic rats (AIDRs). The effect of these fractions (200 mg/kg body weight i.p) on fasting blood glucose (FBG), total cholesterol (TC), triglyceride (TG), serum glutamate oxaloacetate transaminases, serum glutamate pyruvate transaminases (SGOT, SGPT) level, and liver glycogen content were investigated in AIDRs and found significant effects. The most significant reduction of FBG level of around 80.19% was observed for Et-Ac fraction in AIDRs. A significant reduction (p< 0.01) in serum TC and TG level of 54.49% and 79.78% respectively was also found for Et-Ac fraction of O. sanctum. The hypoglycemic and hypolipidemic activities were comparable to metformin HCl (150 mg/kg). In severely diabetic rats, liver glycogen content was decreased by 50.60%. Administration of these fractions to the AIDRs resulted in the significant elevation of liver glycogen content. In diabetic rats, SGOT and SGPT levels were significantly elevated that were further reduced after intraperitoneal administration of these fractions. These results indicate that different fractions of O. sanctum have favorable effects in bringing down the severity of diabetes together with hepatoprotectivity.

BackgroundDiabetes mellitus is a global health problem and constantly increasing day by day. The number of diabetic people in world is expected to rise to 366 million in 2030. The available drugs for diabetes, insulin or oral hypoglycemic agents have one or more side effects and search for new antidiabetic drugs with minimal or no side effects from medicinal plants is a challenging for us. The present study was undertaken to investigate the antidiabetic and antioxidant activity of Semecarpus anacardium (Linn.) (abbreviated as SF).MethodsThe antidiabetic activity was determined by using alloxan-induced diabetic rats. After 15 days of treatment, serum biochemical parameters such as TC, TG, LDL, HDL, SGOT and SGPT were estimated. The survival rate, body weight, organ weight, liver glycogen and blood parameters (RBC and Hb) were also measured. The antioxidant activity was measured by DPPH free radical scavenging assay. Phytochemical screening, total phenolic and total flavonoid content were determined by using standard methods.ResultsThe results showed that the survival rate was 100% in rats of Group SA 400. The effect of extract on blood glucose level in Groups SA 100, SA 200 and SA 400 were dose-dependent throughout the treatment period. No significant changes in organ weight to body weight ratio were observed, liver weights significantly improved in Groups SA 200 and SA 400. The bark extract exhibited significant (p < 0.05) anti-diabetic activity with lowering TC, TG, LDL level dose-dependently and protected liver which may be partially explained by attenuation of SGOT and SGPT levels and increases liver glycogen. The percentage of Hb and RBC counts were negatively correlated with the doses of extracts. In DPPH scavenging assay, IC50 values of SA extract and ascorbic acid were found 72.24 μg/ml and 17.81 μg/ml, respectively. Phytochemical screening showed the presence of steroids, triterpenoids, flavonoids, glycosides, saponins, and tannins that were contribute to biological activity.ConclusionsThese results indicated that stem barks of S. anacardium possess strong anti-diabetic and antioxidant potentials and support traditional medicinal use for the treatment of diabetes mellitus and good source for natural antioxidants.

The antidiabetic and hypolipidemic effects of petroleum-ether, ethyl acetate and chloroform fractions from ethanolic extract of the leaves of Catharanthus roseus (C. roseus) were investigated in normal and streptozotocin-induced diabetic rats (SIDRs). Single doses (150 mg/kg, i.p.) of C. roseus extracts in the fasting blood glucose (FBG) levels were determined in normal and SIDRs on 0, 1, 2, 3, 6, 10, 16, and 24 th hours and serum triglyceride (TG) and serum total cholesterol (TC) levels were determined after 24 th hour. In normoglycemic rats and in SIDRs, petroleum-ether and ethyl acetate fraction of C. roseus reduced blood glucose level significantly. In case of hypolipidemic effects, all fractions reduced serum total cholesterol but the ethyl acetate fraction of C. roseus was the most effective. All fractions of C. roseus reduced serum triglyceride level but the ethyl acetate fraction reduced triglyceride level at the highest. The antidiabetic and hypolipidemic activities were compared to metformin HCl (150 mg/kg). Of all the three fractions, ethyl acetate fractions were the best in activity. Ethyl acetate fraction of C. roseus was found to contain flavonoids and alkaloids. The mechanism underlying the antidiabetic activity is probably increased glycogenesis, decreased gluconeogenesis or decreased absorption of glucose from intestine.