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Scope: Shellfish allergy is an important cause of food allergy, and tropomyosin (TM) is the major allergen within shellfish. Probiotics are safe bacteria that benefit host health and nutrition and is proposed as a novel approach for treating immunological diseases, including food allergies. Methods and results: The probiotic strain Lactobacillus casei Zhang (LcZ) isolated from koumiss is investigated for its capacity to modulate food allergyinduced by TM in BALB/c mice. Oral administration of LcZ attenuated allergy symptoms and intestinal epithelial damage. Furthermore, flow cytometry, real-time quantitative PCR, and ELISA demonstrated that LcZ administration altered the development and function of dendritic cells (DCs), T cells, and B cells, finally resulting in the change of TM-specific antibody isotypes into a tolerogenic pattern. Moreover, an in vitro spleen cell culture model reveals that LcZ directly modulates regulatory tolerogenic DC and T cell development, dependent on the activation of the nuclear factor kappa B (NF-B) signaling pathway. Conclusion: This work indicates the ability of LcZ to alleviate TM-induced food allergy and demonstrates the involvement of the tolerogenic immune cells and NF-B signaling pathway, indicating LcZ to be a potential immunomodulator and immunotherapy assistor.

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Porphyra polysaccharides possess multiple pharmacological activities, such as immunoregulatory, anti-tumor and anti-inflammatory effects, but the specific underlying mechanisms are not fully understood.

Fbxo6 (also called FBG2) is a critical component of the evolutionarily conserved ubiquitin–protein ligase complex SCF (Skp1/Cdc53‐Cullin1/F‐box). Previous studies have demonstrated that Fbxo6 facilitates the growth and proliferation but inhibits the apoptosis and invasion of gastric cancer cells. However, the role of Fbxo6 in non‐small cell lung cancer (NSCLC) is still not clear. Our results revealed that Fbxo6 expression is correlated with early TNM stage and favorable overall survival of NSCLC patients. Further in vitro experiments showed that Fbxo6 inhibits proliferation, facilitates apoptosis and promotes the sensitivity of cisplatin via decreased expression and phosphorylation of Chk1. Thus, Fbxo6 may be a useful prognosis marker and therapeutic target to overcome the chemoresistance of cisplatin‐based chemotherapy agents in NSCLC patients.

Food allergy is a severe food safety problem worldwide. Probiotics are effective in alleviating food allergy whereas antibiotics may exacerbate allergenic responses, but the knowledge on the interplay between them during food allergy is limited. In the present study, we investigate the activity of probiotic strain Lactobacillus casei (Lc) and antibiotic cocktail (Abx) on in a food allergy mouse model. The results showed that Lc relieved allergenic symptom and changed allergen-specific antibodies isotypes. Moreover, Lc upregulated chemokines in the spleen, and promoted regulatory CD103+ DC and IgA-producing B cell proliferation. In contrast, the application of Abx eliminated all these beneficial effects of Lc. Microbiota analysis revealed that the commensal genus Butyricicoccus was a critical mediator in the process. These results revealed the mechanisms of Lc and Abx interaction on food allergy and provided Butyricicoccus as a potential therapeutic target for food allergy.

Purpose: FAM110B is a member of the FAM110 family (family with sequence similarity 110), which is a component of the centrosome associated proteins. Previous studies have shown that FAM110B may be involved in the process of cell cycle and may play a role in carcinogenesis and tumor progression. Using an online database, we found that FAM110B may predict favorable prognosis in non-small cell lung cancer (NSCLC). Therefore, the role of FAM110B playing in NSCLC needs to be further investigated. Patients and Methods: Online databases and immunohistochemistry were used to predict the expression and prognostic value of FAM110B in NSCLC samples. Immunofluorescence staining was used to investigate the subcellular distribution of FAM110B. Western blot, MTT, colony formation, and matrigel invasion assay were used to detect the expression and the effect of FAM110B on mediating proliferation and invasion in NSCLC cell lines. Results: In this study, immunohistochemistry results showed that FAM110B expression significantly correlated with early TNM staging (P=0.020) and negative regional lymph node metastasis (P=0.006). Kaplan-Meier survival analysis found that the median survival time of patients with positive FAM110B expression (56.181±2.348 months) was significantly longer than those with negative FAM110B expression (47.701±2.997 months, P=0.024). Moreover, overexpression of FAM110B inhibited the proliferation and invasion of A549, H1299, and LK2 cell lines. Conversely, FAM110B RNAi exerted opposite effects in the above cell lines. Furthermore, FAM110B overexpression downregulated the active β-catenin, phosphorylation of GSK-3β (p-GSK-3β), cyclin B1, cyclin D1, MMP2, and MMP7, and upregulated the phosphorylation of β-catenin (p-β-catenin) in A549 and H1299 cells. Besides, the FAM110Binduced depressions of p-GSK-3β and active β-catenin were reversed after being treated with Wnt/β-catenin inhibitor, XAV-939. Conclusion: In summary, our results demonstrated that the overexpression of FAM110B restricts the proliferation and invasion of NSCLC cells by inhibiting Wnt/β-catenin signaling. Our study reveals the antitumor function of FAM110B in NSCLC and indicates that FAM110B is a potential therapeutic target.

Mol. Nutr. Food Res. 2020, 64, 1900496 DOI: https://doi.org/10.1002/mnfr.201900496 By using a mouse model, Yanbo Wang and co‐workers demonstrate that probiotic Lactobacillus casei Zhang (LcZ) alleviates shrimp tropomyosin‐induced food allergy via maintaining epithelia stability and immune cell homeostasis, which was mediated by NF‐κB signaling. The study, described in article 1900496, suggests LcZ to be a promising immunomodulator and immunotherapy assistor, especially for food allergy.