Application of in vitro and in vivo models in the study of food allergy

Huang, Jinsha; Liu, Changjun; Li, Han; Wang, Chong; Xie, Menghua; Qian, Yi; Fu, Linglin

doi:10.1016/j.fshw.2018.10.002

Cited by 42 publications

(32 citation statements)

References 73 publications

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“…To identify the possible immune mechanisms associated with allergic reactions observed in zebrafish in response to tick saliva and red meat consumption, selected immune response and food allergy markers involved in T H 1 and T H 2 cellmediated responses (Lu et al, 2008;Huang et al, 2018) were characterized in the kidney and intestine involved in both innate and adaptive fish immunity (Liu et al, 2015;Brugman, 2016;Martins et al, 2019). The CD4 + T cells or T helper cells develop into T H 1 and T H 2 cells ( Figure 10A).…”

Section: Discussionmentioning

confidence: 99%

“…Total RNA was extracted from zebrafish intestine and kidney samples using the AllPrep DNA/RNA/Protein (Qiagen, Hilden, Germany). The expression of selected zebrafish immune response and food allergy markers (Lu et al, 2008;Huang et al, 2018) akirin 2 (akr2), complement component c3a (c3a), interleukin 1-beta (il1b), interleukin 4 (il4), nuclear factor interleukin 3 regulated (nfil3), Toll-like receptor 4b (tlr4b), interferon-induced GTP-binding protein MxA (mxa), interferon (ifn), and MYD88 innate immune signal transduction adaptor (myd88) was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) with gene-specific primers ( Supplementary Table 1) using the KAPA SYBR FAST one-step universal kit (Sigma-Aldrich) in the Rotor-Gene Q (Qiagen) thermocycler following manufacturer's recommendations. A dissociation curve was run at the end of the reactions to ensure that only one amplicon was formed and that the amplicon denatured consistently in the same temperature range for every sample (Ririe et al, 1997).…”

Section: Expression Of Selected Immune Response Markers By Quantitatimentioning

confidence: 99%

“…Additionally, this animal model has been used to study the antibody response to the carbohydrate α-Gal and its potential for the control of infectious diseases caused by pathogens with this modification on their surface (Yilmaz et al, 2014;Cabezas-Cruz et al, 2016;Iniguez et al, 2017;Moura et al, 2017;Portillo et al, 2019). In this context, various fish species constitute models for investigating human diseases (Schartl, 2014), and zebrafish (Danio rerio Hamilton 1822) is a relevant animal model for research in genetics, developmental biology, toxicology, oncology, immunology, and allergy (Huang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Allergic Reactions and Immunity in Response to Tick Salivary Biogenic Substances and Red Meat Consumption in the Zebrafish Model

Contreras

Pacheco

Alberdi

et al. 2020

Front. Cell. Infect. Microbiol.

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Ticks are arthropod ectoparasite vectors of pathogens and the cause of allergic reactions affecting human health worldwide. In humans, tick bites can induce high levels of immunoglobulin E antibodies against the carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) present in glycoproteins and glycolipids from tick saliva that mediate anaphylactic reactions known as the alpha-Gal syndrome (AGS) or red meat allergy. In this study, a new animal model was developed using zebrafish for the study of allergic reactions and the immune mechanisms in response to tick salivary biogenic substances and red meat consumption. The results showed allergic hemorrhagic anaphylactic-type reactions and abnormal behavior patterns likely in response to tick salivary toxic and anticoagulant biogenic compounds different from α-Gal. However, the results showed that only zebrafish previously exposed to tick saliva developed allergic reactions to red meat consumption with rapid desensitization and tolerance. These allergic reactions were associated with tissue-specific Toll-like receptor-mediated responses in types 1 and 2 T helper cells (T H 1 and T H 2) with a possible role for basophils in response to tick saliva. These results support previously proposed immune mechanisms triggering the AGS and provided evidence for new mechanisms also potentially involved in the AGS. These results support the use of the zebrafish animal model for the study of the AGS and other tick-borne allergies.

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Section: Discussionmentioning

confidence: 99%

Section: Expression Of Selected Immune Response Markers By Quantitatimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Allergic Reactions and Immunity in Response to Tick Salivary Biogenic Substances and Red Meat Consumption in the Zebrafish Model

Contreras

Pacheco

Alberdi

et al. 2020

Front. Cell. Infect. Microbiol.

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“…The animal models are adequate as long as they have a GI system similar to the one of humans, produce IgE antibodies and manifest similar allergic reactions in response to food allergens. Among rodent models, Brown Norway and Wistar rats meet these conditions [ 15 ]. Although Brown Norway rats have been traditionally claimed as the most suitable model to study FA, Wistar rats have recently been demonstrated to increase IgE levels after oral immunization with OVA and to develop the key clinical manifestations of FA to peanut allergens [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Glycomacropeptide on Food Allergy with Gastrointestinal Manifestations in a Rat Model through Down-Regulation of Type 2 Immune Response

et al. 2020

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Glycomacropeptide (GMP) is a bioactive peptide derived from milk κ-casein with immune-modulatory and anti-inflammatory properties. Food allergy (FA) is an adverse immune reaction with a broad spectrum of manifestations. Allergen intake induces persistent intestinal inflammation and tissue damage. In this study, the anti-allergic activity of GMP was evaluated using a rat ovalbumin (OVA)-induced FA model with gastrointestinal manifestation. Rats were orally GMP treated from 3 days prior and during FA development. The severity of food anaphylaxis and diarrheal episodes, antibody production and histamine level were measured. Histopathological changes, inflammation and predominant cytokine profile at intestine were analyzed. Oral GMP intake decreased clinical signs and diarrhea severity induced by allergen, with a significant reduction in intestinal edema and expression level of IL-1β and TNF-α. Prophylaxis with GMP also diminished serum anti-OVA IgE and IgG1, and histamine levels. GMP treatment markedly decreased eosinophil infiltration, mast cell and goblet cell hyperplasia, total IgE expression in intestine, and prevented histological changes in villi, crypts and internal muscularis layer. The treatment effectively suppressed IL-5, IL-13 and GATA3 expression and skewed the intestinal cytokine profile toward type 1 and regulatory. These results suggest that GMP may protect against FA through down-regulating the type 2 inflammatory response.

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“…Often, biopsies from allergic patients or animal's lung or gut are used (4); however, obtaining these specimens can be limiting the research opportunities. In vitro allergy models often consist of single-cell models, such as mast cells (MCs) or basophils (5). MCs or basophils represent cells of the allergic effector phase because their activation results in the acute allergic symptoms, meaning that in these models, allergy treatment can be studied in vitro rather than allergy prevention.…”

Section: Introductionmentioning

confidence: 99%

Specific Polyunsaturated Fatty Acids Can Modulate in vitro Human moDC2s and Subsequent Th2 Cytokine Release

et al. 2020

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Allergy is becoming a rapidly increasing problem worldwide, and in vitro models are frequently used to study the mechanisms behind the different types of allergic response. The dendritic cell (DC)-T-cell model can be used to study sensitization. However, lipopolysaccharide (LPS) is often used to maturate the DCs, but it gives rise to a DC1 phenotype, whereas Th2-driven inflammatory diseases such as allergy are characterized by the involvement of the DC2 phenotype. Our aim was to create a DC2-T-cell human model (human moDC2s) to study in vitro sensitization and validate the model using polyunsaturated fatty acids (PUFAs) that were previously shown to have immunomodulatory properties. We found that the generated DC2s expressed OX40L and drove naive T-cells into IL-13 production of CD4 + effector T-cells. In line with in vivo findings, n−3 long-chain (LC)PUFA docosahexaenoic acid (DHA) effectively decreased the DC2's surface expression of OX40L, as well as the IL-12p40 and IL-23 cytokine production by DC2s and subsequently lowered IL-13 production by DC2-induced effector T-cells. Similar cytokine production effects were found with eicosapentaenoic acid (EPA) and arachidonic acid (AA), whereas linoleic acid (LA) increased OX40L surface expression and subsequent T-cell-derived IL-13/IFNγ ratios, suggesting an increased risk of allergy development. Altogether, these data show that human moDC2s are able to induce Th2-type IL-13 secretion by T-cell differentiated in the presence of these DC2s and that this model can be differentially modulated by PUFAs. These results are in line with previous in vivo studies using PUFAs, indicating that this model may be of use to predict in vivo outcomes.

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Application of in vitro and in vivo models in the study of food allergy

Cited by 42 publications

References 73 publications

Allergic Reactions and Immunity in Response to Tick Salivary Biogenic Substances and Red Meat Consumption in the Zebrafish Model

Allergic Reactions and Immunity in Response to Tick Salivary Biogenic Substances and Red Meat Consumption in the Zebrafish Model

Protective Effect of Glycomacropeptide on Food Allergy with Gastrointestinal Manifestations in a Rat Model through Down-Regulation of Type 2 Immune Response

Specific Polyunsaturated Fatty Acids Can Modulate in vitro Human moDC2s and Subsequent Th2 Cytokine Release

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