Acalypha indica Linn is a tropical weed, grows annually in East Nusa Tenggara. The weed is member of Euphorbiaceae family, a largest plant family known as medicinal plant. The weed leaves were used by local people in NTT to treat wounds, diseases or myasis on their livestock. This study aim to investigate the secondary metabolites in A. indica L leaves as a scientific proven for its local use.
The extract preparation comprises of several steps, i.e collection of fresh leaves, dry and wet sortation. The clean leaves were air dried in a room temperature for about 2 weeks before grounded into powder and subjected to extraction. The extraction method was maceration with ethanol 96% as solvent. The dense extract was evaporated using rotary evaporator and subjected to phytochemical screening.
The result shows that ethanol extract of A.indica leaves were tested positive for flavonoid and tannin. Alkaloid, saponins, triterpenes and steroid were tested negative on the extract.
Regenerasi dan pemulihan jaringan luka merupakan proses fisiologi normal tubuh dalam merespon perlukaan untuk mengembalikan integritas dan fungsi normal kulit. Kesembuhan luka dapat dipercepat dengan pemberian obat kimia maupun obat alami. Salep betadine 10% adalah obat kimia yang sering digunakan dalam perawatan luka untuk menstimulasi penyembuhan luka. Sedangkan, tanaman anting – anting (Acalypha indica) diketahui memiliki aktivitas antiinflamasi, antibakteri, antioksidan, dan antiulser. Daun anting – anting telah lama digunakan oleh masyarakat lokal di NTT untuk mengobati luka dan gangguan kulit lainnya pada ternak peliharaannya. Penelitian ini bertujuan untuk mengetahui gambaran makroskopik dan jumlah fibroblas pada kesembuhan luka insisi pada mencit yang diberi terapi salep ekstrak etanol daun anting – anting (EEDAA), sekaligus sebagai bukti empiris fitofarmaka ini bagi masyarakat lokal di NTT. Penelitian ini bersifat eksperimental laboratorik, menggunakan 30 ekor mencit jantan sehat, berumur 3-4 bulan dengan berat badan berkisar 30-40g. Mencit dibagi dalam 5 kelompok yaitu kelompok kontrol negatif (KN) dan positif (KP) yang masing – masing diaplikasikan basis salep vaselin album dan salep betadine 10%; serta tiga kelompok perlakuan yang masing – masing diberikan salep EEDAA konsentrasi 5%, 10% dan 20%. Setiap kelompok dibuat luka sayat pada area dorsum, dengan ukuran panjang 1.5 cm dengan kedalaman 2 mm. Bahan topikal diberikan sebanyak 2 kali/hari. Pengampilan sampel jaringan luka dilakukan pada hari ke-3, ke-7 dan ke-14. Hasil penelitian menunjukkan bahwa pemberian salep EEDAA konsentrasi 10% menunjukkan kesembuhan luka yang lebih cepat dan optimal dibandingkan dengan salep betadine 10%, salep EEDAA 5% dan 20%.
The Culex mosquito is a species that can creates health problems for humans and animals. The handling efforts of the vector is very important to reduce the impact caused by this vector. One of the plants that has the potential as a larvicide is soursop leaves (Annona muricata L.). This study aims to determine the effect of soursop leaves (Annona muricata L.) extract on the mortality of Culex sp larvae and LC50 value to killing 50% Culex sp larva. The study was conducted from March to June 2020. This study used 7 treatment groups which 5 groups tested the effectiveness of soursop leaves extract and 2 control groups. The research data was analyzed using the Probit test to determine the LC50. The results showed that soursop leaves extract (Annona muricata L.) was effective in killing Culex sp larvae with LC50 value is 0.736%.
Chromolaena odorata, a member of Asteraceae, is a tropical shrub that grows throughout the year and can dominate pastures. The tropical weed is known as a medicinal plant and has the potential as a natural insecticide. For local people in NTT, the crude extract of the leaves is used to treat myasis on the livestock. C. odorata is also used as silage or food alternative for livestock. This study aims to determine the content of bioactive compounds in C. odorata leaves and determine the extract yield value, as scientific validation of local wisdom in NTT. This research began with the collection of fresh C. odorata leaves, extraction by maceration using 70% ethanol as solvent, solvent evaporation, and phytochemical screening. Phytochemical screening showed that the leaf extract of C. odorata from Kupang City contained high amounts of flavonoids, tannins, alkaloids, triterpenes and steroids and were tested negative for saponins. The extract yield value is 6.09%, means that the extract contains bioactive compunds in high concentration. Further research needs to be done to find out in more detail the content of secondary metabolites from C. odorata leaves and its activity in promote wound healing on livestock in NTT.
Brown tick, Rhicephalus sanguineus generally known as a very adaptive tick, is widely spread on tropical, subtropical, and temperate regions, and infested dogs living on not only rural but urban areas. Synthetic acaricides are the most widely control measures. However, with the development of tick resistance, plant-derived components are highly investigated. The present study aims to investigate the activity of Crecentia cujete Linn. leaves extract on R. sanguineus. Fifty adult R. sanguineus were collected from local dogs and used in this study. Ticks are divided into the control and treatment groups. Aquadest and deltamethrin were sprayed to ticks in the negative and positive control groups, respectively. The three treatment groups were given maja leaves extract of 2.5%, 5%, and 10%. The tick mortality was observed for 12 hours of exposure to the extracts. All extracts exhibited similar lethal effects on R. sanguineus with total mortality (100%) after 4 hours exposure, compared to the standard therapy. The highest extract concentration (10%) reduces ticks faster compare to other concentrations. Thus, this study suggests that strong acaricidal activity and mortality rate was dose-dependent. This finding needs further scientific investigation to prove.
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