Profil Fitokimia Ekstrak Etanol Daun Anting – Anting (Acalypha Indica Linn) Di Kota Kupang, NTT

Abstract

Acalypha indica Linn is a tropical weed, grows annually in East Nusa Tenggara. The weed is member of Euphorbiaceae family, a largest plant family known as medicinal plant. The weed leaves were used by local people in NTT to treat wounds, diseases or myasis on their livestock. This study aim to investigate the secondary metabolites in A. indica L leaves as a scientific proven for its local use. 
 The extract preparation comprises of several steps, i.e collection of fresh leaves, dry and wet sortation. The clean leaves were air dried in a room temperature for about 2 weeks before grounded into powder and subjected to extraction. The extraction method was maceration with ethanol 96% as solvent. The dense extract was evaporated using rotary evaporator and subjected to phytochemical screening.
 The result shows that ethanol extract of A.indica leaves were tested positive for flavonoid and tannin. Alkaloid, saponins, triterpenes and steroid were tested negative on the extract.

In vitro anthelmintic activity of Chaetomorpha vieillardii ethanolic extract against adult worm motility and egg-hatching of Haemonchus contortus from sheep

“…In this study, the ethanol extract of C. vieillardii at levels of 2, 4, and 6 mg/mL demonstrated its anthelmintic activity against H. contortus and was able to eradicate 50% of H. contortus worms (LD 50 ) respectively more than 4, 5, and 6 hours faster than the negative control (Table 5). Typically, H. contortus worms can survive for more than 6 hours in 0.9% sodium chloride (Laut et al, 2022), in fact, 24 hours in this study, as well as other control media such as Phosphate Buffer Saline (PBS) and distilled water (Medeiros et al, 2020). However, our study findings indicated a unique observation that at P1, the LD 100 lasted longer compared to P0 (Figure 2).…”

“…However, our study findings indicated a unique observation that at P1, the LD 100 lasted longer compared to P0 (Figure 2). The efficacy of albendazole, as a commonly used synthetic anthelmintic for positive control in in vitro studies (Sakti et al, 2018;Laut et al, 2022) was observed to emerge after the fifth hour of observation. This is different from the study by Sakti et al (2018), where albendazole was observed to begin decreasing the motility of H. contortus from the second hour of observation, consistent with the findings of Sakti et al (2020) at the same concentration of 2 mg/mL and Laut et al (2022) at the 1% (b/v).…”

“…The efficacy of albendazole, as a commonly used synthetic anthelmintic for positive control in in vitro studies (Sakti et al, 2018;Laut et al, 2022) was observed to emerge after the fifth hour of observation. This is different from the study by Sakti et al (2018), where albendazole was observed to begin decreasing the motility of H. contortus from the second hour of observation, consistent with the findings of Sakti et al (2020) at the same concentration of 2 mg/mL and Laut et al (2022) at the 1% (b/v). The longer time for the efficacy of albendazole may be attributed to the increasing severity of resistance, low drug availability in plasma, and the high occurrence of resistant genes (Coles et al, 2006;Jabbar et al, 2022), although from the perspective of the host animal, genetic selection can be performed to obtain a more parasite-resistant breed (Niciura et al, 2022).…”