African swine fever (ASF) is a destructive re-emerging swine disease that has posed a serious economic threat to the global pig farming sector. In past years, ASF has rapidly spread over Europe, Asia, and Oceania, and begin to enter Indonesia in the middle of 2019. The clinical and pathological symptoms of ASF are influenced by the strain's virulence, the transmission pathway, and the pig's immunological and health status. ASF’s clinical manifestations are known to evolve, from after an invasion enters a new free region to after the disease has been established in the territory for a longer period. Identifying ASF clinical signs and pathological changes is crucial for a comprehensive and reliable early detection system. The objective of this research is to observe and identify gross pathology in ASF suspect pigs in order to obtain a better understanding of the cause of death. Two dead pigs from a farm in Oebelo village, Kupang regency, Indonesia with a recent history of massive deaths had been examined in this study. The post-mortem results showed that hemorrhagic splenomegaly and hemorrhagic lymphadenitis were the main lesions observed at the examinations. Furthermore, hemorrhages were also found in various internal organs such as the kidneys, liver, and heart. To determine the exact cause of the pigs' deaths, a molecular diagnostic test should be conducted.
The percentage of sperm motility and morphology are important criteria in evaluating the quality of sperm before it is used for artificial insemination (AI). This study was conducted to observe post thawing motility and abnormal morphology of spermatozoa Friesian Holstein (FH). The materials were used 10 straws of FH bulls in the form of 0.25 ml. A total of 10 straws then divided into two treatment groups of thawing in water at 37°C and water 8°C, respectively. An examination of the motility and morphology of spermatozoa abnormalities performed every two hours for two times. Calculating the percentage of sperm motility was done by calculating the percentage of spermatozoa moving forward in the field of view under a microscope with a magnification of 10x. the percentage of abnormal spermatozoa was assessed by William's stain. Spermatozoa morphology was observed by using a microscope magnification of 100x. Abnormalities of spermatozoa were calculated from a total of 200 spermatozoa, either normal or abnormal. At the same thawing time, the motility of FH cattle sperms post thawing in water temperature 37°C had a higher preference than that of post thawing in water temperature 8°C, although it was not significantly different (P > 0.05). Based on morphological aspects, frozen semen used in this study is within the tolerance limit for the total percentage of abnormal sperm morphology between 12% to 23% and normal morphology between 70% to 88%.
Regenerasi dan pemulihan jaringan luka merupakan proses fisiologi normal tubuh dalam merespon perlukaan untuk mengembalikan integritas dan fungsi normal kulit. Kesembuhan luka dapat dipercepat dengan pemberian obat kimia maupun obat alami. Salep betadine 10% adalah obat kimia yang sering digunakan dalam perawatan luka untuk menstimulasi penyembuhan luka. Sedangkan, tanaman anting – anting (Acalypha indica) diketahui memiliki aktivitas antiinflamasi, antibakteri, antioksidan, dan antiulser. Daun anting – anting telah lama digunakan oleh masyarakat lokal di NTT untuk mengobati luka dan gangguan kulit lainnya pada ternak peliharaannya. Penelitian ini bertujuan untuk mengetahui gambaran makroskopik dan jumlah fibroblas pada kesembuhan luka insisi pada mencit yang diberi terapi salep ekstrak etanol daun anting – anting (EEDAA), sekaligus sebagai bukti empiris fitofarmaka ini bagi masyarakat lokal di NTT. Penelitian ini bersifat eksperimental laboratorik, menggunakan 30 ekor mencit jantan sehat, berumur 3-4 bulan dengan berat badan berkisar 30-40g. Mencit dibagi dalam 5 kelompok yaitu kelompok kontrol negatif (KN) dan positif (KP) yang masing – masing diaplikasikan basis salep vaselin album dan salep betadine 10%; serta tiga kelompok perlakuan yang masing – masing diberikan salep EEDAA konsentrasi 5%, 10% dan 20%. Setiap kelompok dibuat luka sayat pada area dorsum, dengan ukuran panjang 1.5 cm dengan kedalaman 2 mm. Bahan topikal diberikan sebanyak 2 kali/hari. Pengampilan sampel jaringan luka dilakukan pada hari ke-3, ke-7 dan ke-14. Hasil penelitian menunjukkan bahwa pemberian salep EEDAA konsentrasi 10% menunjukkan kesembuhan luka yang lebih cepat dan optimal dibandingkan dengan salep betadine 10%, salep EEDAA 5% dan 20%.
Controlling Septicemia epizooticae (SE) through vaccination program has been undertaken in Kupang City. However, numbers of fatal cases are still being reported. The purpose of this study is to measure the antibody titer of Bali cattle after SE vaccination, and to determine the effect of age and sex on antibody titers. The 50 serum samples of SE vaccinated Bali cattle were taken from Alak Sub-district (26 samples) and Maulafa Sub-district (24 samples). The selection of sub-districts in Kupang City was taken in a simple random manner. Those serum samples were examined using the indirect enzyme-linked immunosorbent assay (ELISA) method. Antibody titers against SE is declared to be protective when the antibody titer is above 88 ELISA Unit (EU). Results indicated that average value of cattle antibody titer after the SE vaccination was able to trigger a protective antibody response (> 70 EU), meanwhile ONE WAY ANOVA analysis results showed that there is no significant effect (P> 0.05) of cattle age towards antibody titers. In the same way, the paired t test results did not indicate a difference in the value of antibody titers against the sex of the Bali cattle.
Piometra adalah gangguan reproduksi yang terjadi pada anjing berusia muda dan sangat umum pada anjing berusia lebih dari 8 tahun. Pada tanggal 14 Februari 2019 seekor anjing Golden Retriever berjenis kelamin betina, berusia 8 tahun bernama Chelsea dibawa pemilik ke tempat Praktik Dokter Hewan Bersama (PDHB) Grace Vet Care. Riwayat kasus anjing Chelsea 1 x beranak pada tahun 2016 dengan kelahiran eutokia/normal, tidak ada riwayat operasi ovariahisterektomi dan gejala klinis berupa discharge berwarna kecoklatan dengan aroma amis menyengat dari vulva, nafsu makan menghilang, muntah, frekuensi pernafasan 92x/menit, frekuensi pulsus/nadi 80 x/menit dan suhu tubuh 38.90C. Pernafasan dengan ritme cepat dan dalam (terengah-engah/panting), palpasi pada kelenjar parotidea kiri mengalami pembengkakan. Auskultasi jantung terdengar cepat dan kuat. Palpasi pada ventrolateral abdomen mengalami distensi tanpa fluktuasi. Hasil pemeriksaan darah menunjukkan terjadinya trombositopenia, neutropenia, limfositosis, azotemia, hiperproteinemia, hiperglobulinemia dengan rasio albumin:globulin normal. Berdasarkan hasil pemeriksaan USG transabdominal terlihat pembesaran ukuran uterus dan penurunan echogenitas (anechoic) pada lumen uterus. Berdasarkan riwayat reproduksi dan operasi, observasi klinis, pemeriksaan klinis dan organ reproduksi, hematologi analisis, palpasi abdomen dan diagnosa pencitraan ultrasonografi (USG) anjing Chelsea terdiagnosa piometra terbuka.
Esophageal obstruction is a condition that is commonly experienced by dogs and causes disruption to the mobilization of food and water to the stomatch. The aim of this study is to provide information on the management of esophageal obstruction in a dog based on the type and location of the obstruction. The material used in this case study is a Labrador retriever dog, female, 2 years old, brownish red and weighing 24 kg. Based on history, clinical symptoms and radiographic examination, the diagnosis of this case was obstruction of a foreign body in intraluminal esophagus. Before treatment, the dog was anesthetized by premedication Atropine sulfate at 0.02 mg/kg body weight sub-cutaneously, and induction of anesthesia through combination injection of Ketamin HCL dose 10 mg/kg body weight and Xylazine dose 2 mg/ kg body weight intramuscularly. Goal treatment that has been done in this case is taking a foreign body in the form of a piece cow bone in intraluminal esophagus through the oral cavity.
Cryptorrchid is the most common congenital defect found in dogs and cats. Cryptorrchid is a failure condition of one or both testicle to descend into the scrotum. On 2nd March 2019 an examination and treatment of two bilateral cryptorrchid’s dogs were done at the Praktik Dokter Hewan Bersama Grace Vetcare. The first patient’s identity is domestic dog, 3 years old, brown hairs, weigh 13 kg called name Iro. The second patient’s identity is domestic dog, 1.5 years old, brown hairs, weigh 15.5 kg called name Jacky. Based on physical examination and ultrasound examination, two dogs were diagnosed Bilateral Inguinal Cryptorrchid. Management treatment of both patients were done by orchiectomy operation. Orchiectomies were done by an open method, which is making incision in the left and right lateral inguinal region of the penis. This operation was done to minimize the risk of sertoli cell tumor (sertolioma). Orchiectomy is the only medical procedure that was recommended for cryptorchid treatment.
Conception Rate (CR) and Service Conception (S/C) are two parameters for assessing the reproductive performance of cattle. This research was conducted to determine the value of S/C and CR in crossbreeding cattle in the working area of the Public Health Center. The material used in this study was 72 crossbreed cattle aged 3-10 years. Semi-intensive rearing system with AI mating. The results the S/C in this study was 3.9 higher than the ideal standard of 1.6–2. The CR in this study was 58.6%. CR numbers are still relatively low from the ideal, namely 65 -75%.
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