Globally
growing problems related to cardiovascular diseases lead to a considerable
need for synthetic vascular grafts. For small-caliber vascular prosthesis,
it remains essential to fulfill rapid endothelialization, inhibit
intimal hyperplasia, and prevent calcification for keeping patency.
To modulate vascular regeneration, herein, we developed a bioactive
trilayered tissue-engineered vascular graft encapsulating both microRNA-126
and microRNA-145 in the fibrous inner and middle layers, respectively.
In vitro cell activities demonstrated that the trilayered electrospun
membranes had significant biological advantages in enhanced growth
and intracellular nitric oxide production of vascular endothelial
cells, modulation of phenotypes of vascular smooth muscle cells (SMCs),
and restraint of calcium deposition through fast-releasing microRNA-126
and slow-releasing microRNA-145. Histological and immunofluorescent
analyses of in vivo implantation in a rat abdominal aorta interposition
model suggested that the dual-microRNA-loading trilayered electrospun
graft exerted a positive effect on accelerating endothelialization,
improving contractile SMC regeneration, and promoting normal extracellular
matrix formation. Meanwhile, the local bioactivity of microRNA-126
and microRNA-145 in the trilayered vascular graft could regulate inflammation
and depress calcification possibly by facilitating transformation
of macrophages into the anti-inflammatory M2 phenotype. These findings
indicated that the trilayered electrospun graft by local delivery
of dual microRNAs could be possibly used as a bioactive substitute
for replacement of artificial small-caliber blood vessels.
The development of low carbon economy is closely related to the interests of all sectors of society, especially the main three to participate: the government, enterprises and the public. In this process, how to coordinate the interests of all parties and formulate a reasonable incentive mechanism has become an important task for the government. Studied on the analysis of the behavior status and goals of the government, enterprises and public on the background of low carbon economic development, then used the game method to quantify each object' interests of the target and relations. At last, designed incentive mechanism on the basis of incentive compatibility principle to meet the individual subject welfare maximization and, the greatest degree of protection for the development of low carbon economy.
As tissue regeneration material, electrospun fibers can mimic the microscale and nanoscale structure of the natural extracellular matrix (ECM), which provides a basis for cell growth and achieves organic integration with surrounding tissues. At present, the challenge for researchers is to develop a bionic scaffold for the regeneration of the wound area. In this paper, polyurethane (PU) is a working basis for the subsequent construction of tissue-engineered skin. poly(L-lactide-co-caprolactone) (PLCL)/carboxymethyl chitosan (CMCS) composite fibers were prepared via electrospinning and cross-linked by glutaraldehyde. The effect of CMCS content on the surface morphology, mechanical properties, hydrophilicity, swelling degree, and cytocompatibility were explored, aiming to assess the possibility of composite scaffolds for tissue engineering applications. The results showed that randomly arranged electrospun fibers presented a smooth surface. All scaffolds exhibited sufficient tensile strength (5.30–5.60 MPa), Young’s modulus (2.62–4.29 MPa), and swelling degree for wound treatment. The addition of CMCS improved the hydrophilicity and cytocompatibility of the scaffolds.
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