Synthesis and Characterization of PU/PLCL/CMCS Electrospun Scaffolds for Skin Tissue Engineering

Abstract

As tissue regeneration material, electrospun fibers can mimic the microscale and nanoscale structure of the natural extracellular matrix (ECM), which provides a basis for cell growth and achieves organic integration with surrounding tissues. At present, the challenge for researchers is to develop a bionic scaffold for the regeneration of the wound area. In this paper, polyurethane (PU) is a working basis for the subsequent construction of tissue-engineered skin. poly(L-lactide-co-caprolactone) (PLCL)/carboxymethyl chitosan (CMCS) composite fibers were prepared via electrospinning and cross-linked by glutaraldehyde. The effect of CMCS content on the surface morphology, mechanical properties, hydrophilicity, swelling degree, and cytocompatibility were explored, aiming to assess the possibility of composite scaffolds for tissue engineering applications. The results showed that randomly arranged electrospun fibers presented a smooth surface. All scaffolds exhibited sufficient tensile strength (5.30–5.60 MPa), Young’s modulus (2.62–4.29 MPa), and swelling degree for wound treatment. The addition of CMCS improved the hydrophilicity and cytocompatibility of the scaffolds.

Hydrogel Loaded with Peptide-Containing Nanocomplexes: Symphonic Cooperation of Photothermal Antimicrobial Nanoparticles and Prohealing Peptides for the Treatment of Infected Wounds

“…The MNPs/Cy RL‑QN15 /FeCMCS hydrogel system contains multiple antibacterial components, including amino and carboxyl groups in CMCS, as well as phenolic hydroxyl groups in MNPs, which are known for their antibacterial properties. Additionally, the MNPs exhibit a strong photothermal conversion ability upon receiving 808 nm NIR, leading to the destruction of bacterial cell membranes and the release of their contents, thereby achieving sterilization. ,, …”

“…Additionally, the MNPs exhibit a strong photothermal conversion ability upon receiving 808 nm NIR, leading to the destruction of bacterial cell membranes and the release of their contents, thereby achieving sterilization. 30,58,59 Free radicals can hinder tissue regeneration by selectively targeting and damaging DNA and proteins. 60 The antioxidant potential of the hydrogel was evaluated through the scavenging of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS + ) and hydroxyl radicals ( • OH).…”

Fibroblastic tissue growth on polymeric electrospun membranes: a feasibility study

“…It is known to be highly biocompatible due to its cell-binding motifs such as, RGD and porous structure that closely resembles natural ECM [36] Additional biomaterials that are used in tissue engineering include Polyurethane (PU) and Polyvinyl Alcohol (PVA) for their low-cost fabrication, biocompatibility, and advantageous mechanical properties [37,38]. Similar to PCL, PU is a linear polymer with an analogous functional group and is known to increase cellular attachment [39]. PVA is a vinyl polymer used as the backbone of tissues such as bone, vascular and neural due to its mechanical performance, biocompatibility, and wettability [38].…”

In Vitro Effects of Waterborne Polyurethane 3D Scaffolds Containing Poly(lactic-co-glycolic acid)s of Different Lactic Acid/Glycolic Acid Ratios on the Inflammatory Response

“…Many materials have been applied to the synthesis of tissue engineering scaffolds including natural and synthetic materials [ 20 ]. The most-used natural materials for this purpose include gelatin [ 21 ], collagen [ 22 ], sodium alginate [ 23 ], etc., and the most-used synthetic biomaterials include polycaprolactone (PCL) [ 24 ], polycarbonate diol (PCDL) [ 25 ], polyurethane (PU) [ 26 ], etc. Among these materials, polyurethane, which has gained significant attention due to its good biocompatibility and adjustability, is a versatile biomaterial [ 27 ].…”