Electrospun membranes of PELCL/PCL-REDV loading with miRNA-126 for enhancement of vascular endothelial cell adhesion and proliferation

Zhou, Fang; Wen, Meiling; Zhou, Peiqiong; Zhao, Yunhui; Jia, Xiaoling; Fan, Yubo; Yuan, Xiaoyan

doi:10.1016/j.msec.2017.12.005

Cited by 46 publications

(35 citation statements)

References 49 publications

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“…Garcia et al [147] showed that functionalization of HA hydrogels with RGD or IKVAV resulted in enhancement of migration and proliferation of mouse myoblasts. Moreover, Zhou et al [148] showed that PCL-based membrane combined with REDV promoted adhesion and proliferation of endothelial cells in vitro. Mann and West [149] fabricated PEG scaffold modified with KQAGDV and demonstrated that such biomaterial enhanced migration, adhesion, and proliferation of smooth muscle cells derived from rat thoracic aorta.…”

Section: Presence Of Pro-adhesive Sequencesmentioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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Polymer scaffolds constitute a very interesting strategy for tissue engineering. Even though they are generally non-toxic, in some cases, they may not provide suitable support for cell adhesion, proliferation, and differentiation, which decelerates tissue regeneration. To improve biological properties, scaffolds are frequently enriched with bioactive molecules, inter alia extracellular matrix proteins, adhesive peptides, growth factors, hormones, and cytokines. Although there are many papers describing synthesis and properties of polymer scaffolds enriched with proteins or peptides, few reviews comprehensively summarize these bioactive molecules. Thus, this review presents the current knowledge about the most important proteins and peptides used for modification of polymer scaffolds for tissue engineering. This paper also describes the influence of addition of proteins and peptides on physicochemical, mechanical, and biological properties of polymer scaffolds. Moreover, this article sums up the major applications of some biodegradable natural and synthetic polymer scaffolds modified with proteins and peptides, which have been developed within the past five years.

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Section: Presence Of Pro-adhesive Sequencesmentioning

confidence: 99%

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

2020

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“…15,17 Studies on small-diameter vascular grafts with a satisfactory comprehensive performance, especially cells adhesion, cells proliferation, and high compliance, have been performed. [18][19][20] Biomedical hydrogels have been studied in the field of biomaterials because of their porous structure, high water content, and elasticity. 21 Hydrogel-based vascular grafts have also been extensively investigated to develop a vascular graft with high compliance.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous strategies have been developed to improve cells adhesion and proliferation performance, resulting in endothelialization in vitro and in vivo. 12,[14][15][16][17][18][19][20][21][22][23][24][25][26] Shin 27 et al enhanced the endothelialization of poly(L-lactide-co-ε-caprolactone) vascular grafts through polydopamine coating and the immobilization of vascular endothelial growth factors. Inseong 28 et al utilized PDA as an adaptor to graft heparin onto polyurethane substrates, leading to the enhancement of blood compatibility.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels with polydopamine and REDV for improved applicability

Xing

et al. 2019

J Biomed Mater Res

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Developing a small‐diameter vascular graft with a satisfactory performance in terms of mechanical and biological properties remains a challenging issue because of comprehensive requirements from clinical applications. Polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels exhibit many desirable characteristics for small‐diameter vascular grafts because of their tunable mechanical properties, especially high compliance. However, poor cells adhesion hinders their application for endothelialization in situ. Therefore, in the present work, polydopamine (PDA) and tetrapeptide Arg‐Glu‐Asp‐Val (REDV) were used to functionalize the hydrogels surface and improve cells adhesion. A series of characterizations were systematically conducted to examine the applicability of coated hydrogels to small‐diameter vascular grafts. Results showed that bare and coated hydrogels have appropriate structural stability, and no significant differences in tensile properties could be found after being coated with PDA or PDA‐REDV. The hydrophilicity of the hydrogels decreased with the coatings of PDA and especially PDA‐REDV to improve protein adsorption, porcine iliac artery endothelial cells (PIECs) adhesion, viability, proliferation, and spreading on the hydrogels. Lower hemolysis percentages and higher blood clotting index values were attained for the hydrogels, suggesting their satisfactory hemocompatibility. Overall, the present work provided insights into the development of a novel hydrogel‐based small‐diameter vascular graft. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:117–127, 2020.

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“…Different types of Polyurethane have a variety of medical applications, like manufacturing of implants, wound dressings, medical devices and drug carriers, this broad use is due to their excellent physical and mechanical properties and also their acceptable biocompatibility and hemocompatibility [22][23][24]. There are several reports which designed electrospun multi-layer structures to improve drug release from the wound dressings [25][26][27][28]. Thus, in this study, in order to achieve sustained release of chitosan-based mat, Cs/PEO nanofibers without extract and genipin were fabricated on the facial surface of dressing.…”

Section: Introductionmentioning

confidence: 99%

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2019

Biointerface Res Appl Chem

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In the present study, multilayer electrospun chitosan-based dressing containing Semellil extract (ANGIPARS ™) was fabricated and evaluated as wound dressing. The first layer was polyurethane nanofibers which used as a protective layer, the middle layer was chitosan nanofibers loaded with extract and third layer, chitosan mat, was applied to improve sustained release of dressing. In order to increase the stability of dressings in physiological environment genipin, was applied to crosslink the chitosan nanofibers. So the FE-SEM images indicated stability of genipin cross-linked nanofibers after immersion in PBS. In addition, extract release of the mats were considered by UV absorption in determined wavelength (268 nm). Maintaining 84% cell viability was proved that the genipin-cross-linked dressing containing extract, is nontoxic to human skin fibroblasts. Also the full-thickness wound in rat models was used to evaluate the healing effect of the extract-loaded coatings, moreover, histological performance and significant differences between groups were investigated by one-way ANOVA test. The genipin-cross-linked mats containing extract showed more improved and accelerated wound-healing properties than other samples. This result was confirmed by histological examination and these mats had significantly wound-closure percentage of about 94% after 14 days. All these results indicate that chitosan-based electrospun dressings containing Semellil Melilotus Officinalis extract, due to improved wound healing profile, could be used as a wound dressing.

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Electrospun membranes of PELCL/PCL-REDV loading with miRNA-126 for enhancement of vascular endothelial cell adhesion and proliferation

Cited by 46 publications

References 49 publications

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

Proteins and Peptides as Important Modifiers of the Polymer Scaffolds for Tissue Engineering Applications—A Review

Surface modification of polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels with polydopamine and REDV for improved applicability

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