Surface modification of polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels with polydopamine and REDV for improved applicability

Abstract

Developing a small‐diameter vascular graft with a satisfactory performance in terms of mechanical and biological properties remains a challenging issue because of comprehensive requirements from clinical applications. Polyvinyl alcohol (PVA)/polyacrylamide (PAAm) hydrogels exhibit many desirable characteristics for small‐diameter vascular grafts because of their tunable mechanical properties, especially high compliance. However, poor cells adhesion hinders their application for endothelialization in situ. Therefore, in the present work, polydopamine (PDA) and tetrapeptide Arg‐Glu‐Asp‐Val (REDV) were used to functionalize the hydrogels surface and improve cells adhesion. A series of characterizations were systematically conducted to examine the applicability of coated hydrogels to small‐diameter vascular grafts. Results showed that bare and coated hydrogels have appropriate structural stability, and no significant differences in tensile properties could be found after being coated with PDA or PDA‐REDV. The hydrophilicity of the hydrogels decreased with the coatings of PDA and especially PDA‐REDV to improve protein adsorption, porcine iliac artery endothelial cells (PIECs) adhesion, viability, proliferation, and spreading on the hydrogels. Lower hemolysis percentages and higher blood clotting index values were attained for the hydrogels, suggesting their satisfactory hemocompatibility. Overall, the present work provided insights into the development of a novel hydrogel‐based small‐diameter vascular graft. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:117–127, 2020.

RETRACTED: Amelioration of imiquimod-induced psoriasis-like dermatitis in mice by DSW therapy inspired hydrogel

“…The cells on the hydrogels were stained with Thermo Scientific HERAcell (150i, U.S.A.) and then photographed by laser scanning confocal microscope using (TCSSP5, Leica, Germany). As described in previous work, CCK8 method was used to quantify the cell growth on hydrogels [ 43 ].…”

Rational Design of Thermoresponsive Microgel Templates with Polydopamine Surface Coating for Microtissue Applications

“…The black coloration of PDA‐coated hydrogels is described in literature and thus a good indication of the successful hydrogel coating. [ 24 ] To further examine that coating, the hydrogels are freeze dried and subsequently analyzed by Fourier‐Transform Infrared spectroscopy (FT‐IR) using an FT‐IR‐470 instrument (JASCO Analytical Instruments), along with a reference based on pure PDA (Figure 6a, left). The spectrum of PDA.0 has characteristic signals at 3431 cm −1 (OH and NH stretching mode), 2840–3000 cm −1 (CH stretching modes), and two signals at 1633 cm −1 (C═O stretching mode) and 1538 cm −1 (NH bending mode).…”

Investigation of human adipose‐derived stem‐cell behavior using a cell‐instructive polydopamine‐coated gelatin–alginate hydrogel

“…The increase in cell area and length of cytoskeletal filaments suggests an enhancement in the cell adhesion properties to the substrate, which could be attributed to the increased absorption on the hydrogel surface of serum proteins present in the media promoted by pDA. For instance, in another report, pDA was able to augment the adsorption of bovine serum albumin (BSA) on polyvinyl alcohol and polyacrylamide hydrogels, significantly 45 . Therefore, we investigated whether the amount of protein adsorbed on the surface of the hydrogels was different based on the pDA treatment.…”

“…For instance, in another report, pDA was able to augment the adsorption of bovine serum albumin (BSA) on polyvinyl alcohol and polyacrylamide hydrogels, significantly. 45 Therefore, we investigated whether the amount of protein adsorbed on the surface of the hydrogels was different based on the pDA treatment. However, this change was not statistically significant among the tested groups.…”

“…Besides the hydrogel's intrinsic ability to modulate cellular morphology, pDA treatment can also be used to affect the biological properties of scaffolds. For instance, many studies have explored the use of pDA to covalently link adhesive or osteogenic peptides 45,47 as well as angiogenic growth factors. 48 Due to the presence of catechol units, the pDA treatment can participate in a variety of chemical interactions, including Michael's addition and Schiff base reactions with amine and thiol functional groups of proteins.…”