Investigation of human adipose‐derived stem‐cell behavior using a cell‐instructive polydopamine‐coated gelatin–alginate hydrogel

Abstract

Hydrogels can be fabricated and designed to exert direct control over stem cells' adhesion and differentiation. In this study, we have investigated the use of polydopamine (pDA)‐treatment as a binding platform for bioactive compounds to create a versatile gelatin–alginate (Gel–Alg) hydrogel for tissue engineering applications. Precisely, pDA was used to modify the surface properties of the hydrogel and better control the adhesion and osteogenic differentiation of human adipose‐derived stem cells (hASCs). pDA enabled the adsorption of different types of bioactive molecules, including a model osteoinductive drug (dexamethasone) as well as a model pro‐angiogenic peptide (QK). The pDA treatment efficiently retained the drug and the peptide compared to the untreated hydrogel and proved to be effective in controlling the morphology, cell area, and osteogenic differentiation of hASCs. Overall, the findings of this study confirm the efficacy of pDA treatment as a valuable strategy to modulate the biological properties of biocompatible Gel–Alg hydrogels and further extend their value in regenerative medicine.

Leveraging the advancements in functional biomaterials and scaffold fabrication technologies for chronic wound healing applications

“…Our recently published work developed a polydopaminecoated gelatin-alginate hydrogel as a binding platform for bioactive molecules used for various tissue engineering applications. 43 Polydopamine is extensively investigated as an adhesive material due to numerous catecholic amino acids in its structure that enable it to bind to various surfaces in both wet and saline conditions. [43][44][45] Further, Mooney and his team developed bioinspired active adhesive dressings, shown in Fig.…”

“…43 Polydopamine is extensively investigated as an adhesive material due to numerous catecholic amino acids in its structure that enable it to bind to various surfaces in both wet and saline conditions. [43][44][45] Further, Mooney and his team developed bioinspired active adhesive dressings, shown in Fig. 2A, illustrating the firm adhesion of the hydrogel to the injured skin that was triggered by the changes of the skin temperature, resulting in successful wound contraction.…”

Nanoparticle-Reinforced Tough Hydrogel as a Versatile Platform for Pharmaceutical Drug Delivery: Preparation and in Vitro Characterization

“…The advantage of using PDA is its ability to chemically interact with proteins and biological molecules that contain thiols, amine, hydroxyl, among other nucleophilic groups by Michael addition and Schiff base reaction. 54 It has also been reported that the mussel-inspired PDA is an excellent candidate for tissue attachment. 55 Therefore, we treated GelMA-nSi cryogel with polydopamine to improve the cryogels' bioadhesivity.…”

Controlling Differentiation of Adult Stem Cells Via Cell-Derived Nanoparticles: Implications in Bone Repair

“…Further, we wanted to determine the surface–protein characteristics of the synthesized CDN using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting. Cells express several proteins and receptors on their surface and on the cell membranes, which are responsible for mediating the interaction of the cells with other cells and molecules in the microenvironment surrounding them and within them. , When CDNs are prepared using the membrane fragmentation and cell-shearing approach, these surface proteins and membrane proteins are carried over to the nanoparticles formed. , The presence of one such membrane protein, Caveolin-1, on the CDN was determined using SDS-PAGE and western blotting. Caveolin-1 has a molecular weight of 22 kDa, and it is the primary protein present on caveolae, which are invaginations present on the plasma membrane. , For the western blot, 20 and 40 μg of the CDN Dex and the CDN samples were used.…”

“…Cells express several proteins and receptors on their surface and on the cell membranes, which are responsible for mediating the interaction of the cells with other cells and molecules in the microenvironment surrounding them and within them. 17,22 When CDNs are prepared using the membrane fragmentation and cell-shearing approach, these surface proteins and membrane proteins are carried over to the nanoparticles formed. 17,18 The presence of one such membrane protein, Caveolin-1, on the CDN was determined using SDS-PAGE and western blotting.…”