Modulation of vascular endothelial cells under shear stress on electrospun membranes containing REDV and microRNA-126

Wen, Meiling; Yan, Hongyuan; Shi, Xin; Zhao, Yunhui; Wang, Kai; Yuan, Xiaoyan

doi:10.1080/00914037.2020.1785452

Cited by 7 publications

(6 citation statements)

References 39 publications

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“…In the previous study, perfusion culture was conducted with cells on the single electrospun membrane [31][32][33]. However, electrospun membranes have not been rarely reported as the matrix for the three-dimensional perfusable vessel network in vitro because of their thinness and flexibility.…”

Section: Discussionmentioning

confidence: 99%

Bonding of Flexible Membranes for Perfusable Vascularized Networks Patch

Hong

Song

Choi

et al. 2021

Tissue Eng Regen Med

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BACKGROUND: In vitro generation of three-dimensional vessel network is crucial to investigate and possibly improve vascularization after implantation in vivo. This work has the purpose of engineering complex tissue regeneration of a vascular network including multiple cell-type, an extracellular matrix, and perfusability for clinical application. METHODS: The two electrospun membranes bonded with the vascular network shape are cultured with endothelial cells and medium flow through the engineered vascular network. The flexible membranes are bonded by amine-epoxy reaction and examined the perfusability with fluorescent beads. Also, the perfusion culture for 7 days of the endothelial cells is compared with static culture on the engineered vascular network membrane. RESULTS: The engineered membranes are showed perfusability through the vascular network, and the perfused network resulted in more cell proliferation and variation of the shear stress-related genes expression compared to the static culture. Also, for the generation of the complex vascularized network, pericytes are co-cultured with the engineered vascular network, which results in the Collagen I is expressed on the outer surface of the engineered structure. CONCLUSION: This study is showing the perfusable in vitro engineered vascular network with electrospun membrane. In further, the 3D vascularized network module can be expected as a platform for drug screening and regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Bonding of Flexible Membranes for Perfusable Vascularized Networks Patch

Hong

Song

Choi

et al. 2021

Tissue Eng Regen Med

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“…Furthermore, the capacity of membranes to modulate VECs and VSMCs promoting rapid endothelialization and inhibiting VSMC hyperproliferation was validated by both in vitro and in vivo investigations, proving the feasibility of the proposed approach in tissue regeneration. Recently, Wen et al [94] reported the construction of REDV-functionalized aqueous PELCL core-shell electrospun membranes loaded with microRNA-126 with suitable mechanical properties and prolonged cumulative release of loaded genetic material for the efficient modulation of vascular endothelial cells (VECs).…”

Section: Plasma Treatmentmentioning

confidence: 99%

Electrospun-Fibrous-Architecture-Mediated Non-Viral Gene Therapy Drug Delivery in Regenerative Medicine

2022

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Gene-based therapy represents the latest advancement in medical biotechnology. The principle behind this innovative approach is to introduce genetic material into specific cells and tissues to stimulate or inhibit key signaling pathways. Although enormous progress has been achieved in the field of gene-based therapy, challenges connected to some physiological impediments (e.g., low stability or the inability to pass the cell membrane and to transport to the desired intracellular compartments) still obstruct the exploitation of its full potential in clinical practices. The integration of gene delivery technologies with electrospun fibrous architectures represents a potent strategy that may tackle the problems of stability and local gene delivery, being capable to promote a controlled and proficient release and expression of therapeutic genes in the targeted cells, improving the therapeutic outcomes. This review aims to outline the impact of electrospun-fibrous-architecture-mediated gene therapy drug delivery, and it emphatically discusses the latest advancements in their formulation and the therapeutic outcomes of these systems in different fields of regenerative medicine, along with the main challenges faced towards the translation of promising academic results into tangible products with clinical application.

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“…Functional peptides, fusion proteins, drugs, growth factors, gaseous signal molecules, genes and microRNAs have been used to mediate cell behaviors, especially to accelerate EC adhesion, growth, proliferation and migration, and to inhibit excessive SMC proliferation. 89,90 REDV and VAPG peptides can selectively deliver DNAs and/or microRNAs to ECs and SMCs, and therefore the cell behaviors have been mediated respectively. Thus, the multi-delivery technology can accelerate endothelialization, improve contractile VSMC regeneration, and promote normal ECM formation.…”

Section: Outlook and Perspectivesmentioning

confidence: 99%