Effect of a synthetic Aluminium-Magnesium Silicate (AMS) on Newcastle Disease Virus (NDV) was tested. Equal amounts, of the AMS and of NDV, on a volume to weight (v/w) basis, were incubated for one hour at room temperature, and then centrifuged. The supernatant was tested for NDV titre. Portions of a virulent NDV were similarly incubated with the AMS, and their supernatants tested for morbidity rates on infected chicks. Also, two groups of chicks were infected with virulent NDV intramuscularly (I/m), and by introduction of infected chicks, respectively. Their subgroups were treated by administering AMS through drinking water, to assess its effect on mortality. Incubating NDV with AMS, reduced the viral titre from mean HA, 613 ±86 to 4. 5 ±0.72 (P < 0.05). Also, incubating virulent NDV with the AMS, reduced its morbidity rate from 100% to 20% (P< 0.05) when incubated once, and from 100% to zero, when incubated twice. NDV-infected chicks treated with AMS, had same 100% mortality as the controls, when route of infection was I/m (P > 0.05) but when infection was by introduction of infected chicks, mortality reduced from 20% to zero (P < 0.05).These results suggest AMS as NDV's antiviral agent.
To study effects of vitamins-minerals (VMS) supplementation on immune response of chicks to Newcastle disease vaccination (NDV-LaSota), one hundred broiler chicks were assigned to 4 treatment groups of 25 each. One group was given VMS before the vaccination. Another group was given the supplementation after the vaccination. A third group was given the VMS before and after the vaccination while the fourth group served as vaccinated control. Administration of VMS was in each case, through drinking water. Five randomly selected chicks from each group were bled for sera, used for haemagglutination inhibition (HI)-NDV antibodies test. Three weeks post vaccination, mean NDV antibody titre (1.87±0.18) in chicks given VMS before and after the vaccination was significantly (P < 0.05) higher than 1.39±0.12 of the control but it did not vary (P > 0.05) from 1.81 0.17 and 1.74±0.06, respectively of groups that received the supplementation only before and only after vaccination.
Effects a synthetic Aluminium-Magnesium Silicate [AMS] has on Avian Influenza Virus (AIV) were tested. Equal amounts of AIV samples and of the AMS were mixed, kept one hour at room temperature before centrifuging. The supernatants were remeasured and tested for, viral titre, Mean Death Time (MDT) and Mortality Rate of chicken Embryos (EMR). Volumes of the viral samples reduced at rate of 23.4% ± 5.48%. Viral titres reduced significantly (P < 0.01) from HA, 73 ± 32.72 to 1.4 ± 0.43. Also, EMR of infected chicken embryos reduced from 100% to 65%, while MDT of those that died,increased significantly (P < 0.01) from 76 ± 4.38 to 136 ±18.93 hours. When incubation with AMS was repeated on portions of an AIV sample, MDT increased from 64 to 104 hours with the portion incubated once. AIV portions on which incubation with AMS was repeated could not kill chicken embryos
The efficacy of Levamisole Hydrochloride-a standard immunomodulator was tested in cockerel chicks. Forty day old chicks were allocated into two groups A and B. Group A received Levamisole at the recommended dosage of 10.05mg/20 birds for 3 days prior to the NDV vaccinations (B1, LaSota and Komarov). Group B received all the afore mentioned vaccinations but was not treated with Levamisole. Sera samples were analyzed by Heamagglutination Inhibition (HI) tests. The data obtained were analyzed statistically using paired t-test. High antibody titers against NDV were observed in both groups. There was no significant immunostimulation by Levamisole in this study.
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