The alfalfa plant, after harvesting, was washed, dried, and grinded to get fine powder used in water treatment. We used the alfalfa plant with ethanol to make the alcoholic extract characterized by using (GC-Mass, FTIR, and UV) spectroscopy to determine active compounds. Alcoholic extract was used to prepare zinc nanoparticles. We characterized Zinc nanoparticles using (FTIR, UV, SEM, EDX Zeta potential, XRD, AFM). Zinc nanoparticle with Alfalfa extract and alfalfa powder were used in the treatment of water polluted with inorganic elements such as Cr, Mn, Fe, Cu, Cd, Ag by (Batch processing). The batch process with using alfalfa powder gets treated with Pb (51.45%), which is the highest percentage of treatment. Mn (13.18%), which is the lowest percentage of treatment. The batch process with using Zinc nanoparticles gets the result treated with Pb(98.822%), which is the highest percentage of treatment, and Mn (10.31%), which is the lowest percentage of treatment. When comparing alfalfa powder and zinc nanoparticle, it has been found that the treatment with zinc nanoparticle is more efficient in the removal of inorganic pollutants.
This study aimed to evaluate the effect of cinnamon and turmeric nanoscale extracts on reducing the microbial load of ground beef stored under refrigerated temperature at 2 ° C for 12 days. The study included six different treatments T1 positive control treatment (adding water), T2 negative control treatment (without addition) and T3. (Curcuma 248.3 ppm), T4 (Curcuma 496.9 ppm), T5 (Cinnamon 83.08 ppm) and T6 (Cinnamon 166.16 ppm). The treatments were kept with storage periods of 1, 4, 8 and 12 days, respectively. Some tests were performed to detect microorganisms. The results of the treatments recorded lower values for the total number of bacteria when compared with the two control treatments, and the treatment T6 recorded the lowest values. The results observed that there was a significant decrease (P< 0.05) in the numbers of cold-loving bacteria for the treatments to which the cinnamon and turmeric nanoparticles extract was added. Treatment T6 (cinnamon 166.16 ppm) recorded lowest number of cold-loving bacteria, reaching 4.47, 4.57 and 4.77, 5.22 bacterial units/gm. meat. The results of the added treatments showed a significant decrease (P <0.05) in the logarithm of the number of coliform bacteria compared to the positive control treatments (T1 and T2). In addition, T6 (cinnamon 166.16 ppm) reached lowest number of coliform bacteria and reached 3.69, 3.78, 3.87, 3.99 bacterial units/gm meat. This study concluded that the addition of cinnamon and turmeric nanoparticles extracts to fresh beef and stored by refrigerating for different storage periods. This antibacterial activity assured by a decrease in the logarithm of the total number of normal bacteria, cold-loving bacteria and coliform bacteria, compared with the control treatments for ground beef and cryogenic stock.
Pesticides are vastly used for pests monitoring in agriculture and public health fields, causing severe depletion in quality of drinking water. Moreover, most insecticides resist biodegradation and carcinogen even at very low levels up to Parts per billion (ppb). This study was carried during April/2019 and extended to January/ 2020 in Directorate of Environment and Water, Baghdad-Iraq to synthesis and characterization silver bionanoparticles by using Bacillus thuringiensis israelensis extract (biological methods) and investigate this activity on the chlorpyrifos pesticide under laboratory conditions. The silver bionanoparticles were characterized using many techniques, X-ray Diffractometer (XRD Fourier Transform Infrared (FTIR), atomic force microscopy (AFM) and zeta potential analyzer spectroscopy. The results of XRD technique confirmed the crystalline nature of the nanoparticles. AFM analysis revealed that particles were spherical, single or in aggregates. Determination the shafting of active groups sites was performed using FTIR. The zeta potential values were -36.33 mV for AgNPs. The biodegradation of chlorpyrifos using silver bionanoparticles was determined quantitatively using high performance liquid chromatography (HPLC) techniques. From the result, it can be suggested that silver bio-nanoparticles from Bacillus thuringiensis israelensis extracts lead to biodegradation of chlorpyrifos completely without forming harmful products confirmed by GC-MS analysis. We endorse that this process has scientific potential in the biodegradation of chlorpyrifos pesticide contaminated water using Green biosynthesis of nanoparticles.
Bacillus subtilis, an isolate of bacillus genus, was obtained from the laboratories of Ministry of Science and Technology. The best efficient Bacillus subtilis isolate in cellulose and semi-cellulose hydrolysis was treated with Dielectric-barrier Discharge (DBD). Atmospheric cold plasma technique (non-thermal) was used by exposing them at different times (2, 3, 4 and 5 mins) separately as a first stage, and then 60 seconds after any treatment separately as a second stage. After 48 hours, the difference between the plasma source and the sample was fixed at 0.5 cm. The results showed a variation in the growth of the isolate according to the exposure time by the appearance of culture turbidity and the estimation of optical density. Positive results appeared between exposure times, the amount of optical density, and the cellulose and semi-cellulose decomposition into glucose. Bacillus subtilis increased its efficacy in cellulosic hydrolysis and semi-cellulosic materials. Bacillus subtilis showed malleability and the ability to increase the efficiency in cellulose and semi-cellulose materials hydrolysis. We conducted a new and extensive study by using cold plasma technique to increase the hydrolysis efficacy of food microorganisms.
The research was carried out in the laboratories of the Ministry of Science and Technology/Iraq. Green nanoparticles were prepared using turmeric (Curcuma plant) extracted by using biological methods and were characterized by using some techniques including Scanning Electron Microscopy (SEM), X-ray Diffractometer (XRD) and zeta potential Analyzer. The results showed that the spherical particles were shown individually or in the aggregate, crystalline, and the value zeta voltage at -33.22 mV. The inhibition activity of green nanoparticles was tested against Aspergillus flavus growth and compared with Basten fungicide as a comparison treatment and negative control sample. The results of nanoparticles showed high inhibitor activity in the 590.33 ppm concentration compared to negative control and comparative treatment in concentration 750 ppm. Feeds contaminated with A. flavus isolate were treated with cur. AgNPs at 590.33 ppm for a four-week (incubation period) and Aflatoxin B1. The results showed no Aflatoxin in feed samples were treated with nanoparticles while showing Aflatoxin B1 at a concentration of 38 ppb in control samples. To study the effect of cur. AgNPs against the fungal toxicity in meat broilers feeding to feeding contaminated with Aflatoxin B1 by using some blood and histopathological signs of bird’s liver. The results showed the nanoparticles treatment samples significant superiority compared with the positive control (contaminated with Aflatoxin B1) (p ≤0.01), and no significant differences with control treatment (feed free from any addition). We conclude that the use of green nanoparticles (Curcuma longa L.) produced by biological method as good alternatives to fungicides that harmful to public health and inhibitory activity of A. flavus and prevent the production of Aflatoxin B1 and improve the blood and histopathological signs of birds.
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