Improvement Efficacy of Bacillus Subtilis Cellulose Hydrolyzing by Using Cold Plasma Technique

Abstract

Bacillus subtilis, an isolate of bacillus genus, was obtained from the laboratories of Ministry of Science and Technology. The best efficient Bacillus subtilis isolate in cellulose and semi-cellulose hydrolysis was treated with Dielectric-barrier Discharge (DBD). Atmospheric cold plasma technique (non-thermal) was used by exposing them at different times (2, 3, 4 and 5 mins) separately as a first stage, and then 60 seconds after any treatment separately as a second stage. After 48 hours, the difference between the plasma source and the sample was fixed at 0.5 cm. The results showed a variation in the growth of the isolate according to the exposure time by the appearance of culture turbidity and the estimation of optical density. Positive results appeared between exposure times, the amount of optical density, and the cellulose and semi-cellulose decomposition into glucose. Bacillus subtilis increased its efficacy in cellulosic hydrolysis and semi-cellulosic materials. Bacillus subtilis showed malleability and the ability to increase the efficiency in cellulose and semi-cellulose materials hydrolysis. We conducted a new and extensive study by using cold plasma technique to increase the hydrolysis efficacy of food microorganisms.

Production and characterization of bacterial cellulose utilizing Iraqi vinegar’s mother pellicles

“…use cellulose from different sources . (Hamza et al, 2022;Al-Deresawi et al, 2023). The present study on the production and characterization of bacterial cellulose utilizing Iraqi vinegar's mother pellicles is unique as there is no previous study in Iraq on the production of bacterial cellulose from Iraqi vinegar's mother pellicles using the method used in the present research.…”

Comparison Study Between Crude and Purified L-Asparaginase From Bacillus Spp. for Reduction of Acrylamide Level During Fries Processing

“…Life forms, including animals, plants, and microscopic organisms, all include Lasparaginases (L-asparagine amidohydrolase; EC 3.5.1.1), which catalyze the hydrolysis of Lasparagine on the amide side to aspartic acid and ammonia [1]. The characteristics of Lasparaginases from diverse bacteria have currently been found [2], including those from Erwinia, Pseudomonas spp., Bacillus, Aspergillus, and Escherichia coli [3,4]. For more than 40 years, L-asparaginase has been considered an essential component of a multidrug chemotherapy regimen often used to treat childhood acute lymphoblastic leukemia, Hodgkin's lymphoma, lymphosarcoma, and melanosarcoma [5].…”