The alfalfa plant, after harvesting, was washed, dried, and grinded to get fine powder used in water treatment. We used the alfalfa plant with ethanol to make the alcoholic extract characterized by using (GC-Mass, FTIR, and UV) spectroscopy to determine active compounds. Alcoholic extract was used to prepare zinc nanoparticles. We characterized Zinc nanoparticles using (FTIR, UV, SEM, EDX Zeta potential, XRD, AFM). Zinc nanoparticle with Alfalfa extract and alfalfa powder were used in the treatment of water polluted with inorganic elements such as Cr, Mn, Fe, Cu, Cd, Ag by (Batch processing). The batch process with using alfalfa powder gets treated with Pb (51.45%), which is the highest percentage of treatment. Mn (13.18%), which is the lowest percentage of treatment. The batch process with using Zinc nanoparticles gets the result treated with Pb(98.822%), which is the highest percentage of treatment, and Mn (10.31%), which is the lowest percentage of treatment. When comparing alfalfa powder and zinc nanoparticle, it has been found that the treatment with zinc nanoparticle is more efficient in the removal of inorganic pollutants.
The research was carried out in the laboratories of the Ministry of Science and Technology/Iraq. Green nanoparticles were prepared using turmeric (Curcuma plant) extracted by using biological methods and were characterized by using some techniques including Scanning Electron Microscopy (SEM), X-ray Diffractometer (XRD) and zeta potential Analyzer. The results showed that the spherical particles were shown individually or in the aggregate, crystalline, and the value zeta voltage at -33.22 mV. The inhibition activity of green nanoparticles was tested against Aspergillus flavus growth and compared with Basten fungicide as a comparison treatment and negative control sample. The results of nanoparticles showed high inhibitor activity in the 590.33 ppm concentration compared to negative control and comparative treatment in concentration 750 ppm. Feeds contaminated with A. flavus isolate were treated with cur. AgNPs at 590.33 ppm for a four-week (incubation period) and Aflatoxin B1. The results showed no Aflatoxin in feed samples were treated with nanoparticles while showing Aflatoxin B1 at a concentration of 38 ppb in control samples. To study the effect of cur. AgNPs against the fungal toxicity in meat broilers feeding to feeding contaminated with Aflatoxin B1 by using some blood and histopathological signs of bird’s liver. The results showed the nanoparticles treatment samples significant superiority compared with the positive control (contaminated with Aflatoxin B1) (p ≤0.01), and no significant differences with control treatment (feed free from any addition). We conclude that the use of green nanoparticles (Curcuma longa L.) produced by biological method as good alternatives to fungicides that harmful to public health and inhibitory activity of A. flavus and prevent the production of Aflatoxin B1 and improve the blood and histopathological signs of birds.
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