Biodegradation of chlorpyrifos pesticide using silver bio-nanoparticles Bacillus thuringiensis israelensis extracts

Abstract

Pesticides are vastly used for pests monitoring in agriculture and public health fields, causing severe depletion in quality of drinking water. Moreover, most insecticides resist biodegradation and carcinogen even at very low levels up to Parts per billion (ppb). This study was carried during April/2019 and extended to January/ 2020 in Directorate of Environment and Water, Baghdad-Iraq to synthesis and characterization silver bionanoparticles by using Bacillus thuringiensis israelensis extract (biological methods) and investigate this activity on the chlorpyrifos pesticide under laboratory conditions. The silver bionanoparticles were characterized using many techniques, X-ray Diffractometer (XRD Fourier Transform Infrared (FTIR), atomic force microscopy (AFM) and zeta potential analyzer spectroscopy. The results of XRD technique confirmed the crystalline nature of the nanoparticles. AFM analysis revealed that particles were spherical, single or in aggregates. Determination the shafting of active groups sites was performed using FTIR. The zeta potential values were -36.33 mV for AgNPs. The biodegradation of chlorpyrifos using silver bionanoparticles was determined quantitatively using high performance liquid chromatography (HPLC) techniques. From the result, it can be suggested that silver bio-nanoparticles from Bacillus thuringiensis israelensis extracts lead to biodegradation of chlorpyrifos completely without forming harmful products confirmed by GC-MS analysis. We endorse that this process has scientific potential in the biodegradation of chlorpyrifos pesticide contaminated water using Green biosynthesis of nanoparticles.

Optimization of Growth Conditions for Chlorpyrifos-Degrading Bacteria in Farm Soils in Nakuru County, Kenya

“…By addressing this crucial aspect, the study makes a significant contribution to the field of bioremediation. Moreover, the emphasis on utilizing indigenous species underscores the importance of ecofriendly approaches to tackle pesticide contamination, minimizing potential disruptions to microflora [ 29 , 31 ]. These findings carry profound implications, potentially unlocking targeted and efficient bioremediation strategies for chlorpyrifos-contaminated environments, a critical step towards mitigating the detrimental impacts of this hazardous pesticide.…”

“…Since CP has been used for a long time, several researchers have isolated microbes that degrade the pesticide [20,24,[29][30][31][32][33][34][35][36][37][38][39]. However, only a few studies have tried to optimize the growth conditions for the degrading bacteria.…”

“…However, only a few studies have tried to optimize the growth conditions for the degrading bacteria. tackle pesticide contamination, minimizing potential disruptions to microflora [29,31]. These findings carry profound implications, potentially unlocking targeted and efficient bioremediation strategies for chlorpyrifos-contaminated environments, a critical step towards mitigating the detrimental impacts of this hazardous pesticide.…”

Mechanisms and applications of microbial synthesis of metal nanoparticles in agri-sectors

“…Microbially synthesized MNPs serve both as effective alternatives to pesticides and as a method for degrading pesticides. For example, AgNPs synthesized by the microbe Penicillium pinophilum 270 and Bacillus thuringiensis israelensis 271 can degrade organophosphorus insecticides such as cypermethrin without producing harmful by-products, suitable for treating pesticide-contaminated water bodies. AgNPs biosynthesized from the extract of Bacillus cereus were used to degrade the pesticide malathion.…”