It has been reported that interferon-gamma (IFN-gamma) facilitates differentiation of PC-12 cells and murine adult neural stem cells. Here we show that IFN-gamma promotes the differentiation of C17.2 neural progenitor cells (NPC) into a neuronal phenotype characterized by neurite outgrowth and the expression of the neuronal marker protein beta-III tubulin. IFN-gamma induced an increase in the activity c-jun N-terminal kinase (JNK) without affecting activities of extracellular signal-regulated kinases (ERKs 1 and 2). An inhibitor of JNK blocked the ability of IFN-gamma to promote differentiation of NPC into neurons, whereas an inhibitor of ERKs 1 and 2 did not. Our findings show that the pro-inflammatory cytokine, IFN-gamma has the potential to stimulate neurogenesis, suggesting roles for this cytokine in development and repair of the nervous system.

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SUMMARY Many protein-protein interactions in cells are mediated by functional domains that recognize and bind to motifs containing phosphorylated serine and threonine residues. To create small molecules that inhibit such interactions, we developed methodology for the synthesis of a prodrug that generates a phosphoserine peptidomimetic in cells. For this study, we synthesized a small molecule inhibitor of 14-3-3 proteins that incorporates a nonhydrolyzable difluoromethylene-phosphoserine prodrug moiety. The prodrug is cytotoxic at low micromolar concentrations when applied to cancer cells and induces caspase activation resulting in apoptosis. The prodrug reverses the 14-3-3-mediated inhibition of FOXO3a resulting from its phosphorylation by Akt1 in a concentration-dependent manner that correlates well with its ability to inhibit cell growth. This methodology can be applied to target a variety of proteins containing phosphoserine and other phosphoamino acid binding domains.

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A hard, low-wear probe for contact-mode writing techniques, such as dip-pen nanolithography (DPN), was fabricated using ultrananocrystalline diamond (UNCD). Molding within anisotropically etched and oxidized pyramidal pits in silicon was used to obtain diamond tips with radii down to 30 nm through growth of UNCD films followed by selective etching of the silicon template substrate. The probes were monolithically integrated with diamond cantilevers and subsequently integrated into a chip body obtained by metal electroforming. The probes were characterized in terms of their mechanical properties, wear, and atomic force microscopy imaging capabilities. The developed probes performed exceptionally well in DPN molecular writing/imaging mode. Furthermore, the integration of UNCD films with appropriate substrates and the use of directed microfabrication techniques are particularly suitable for fabrication of one- and two-dimensional arrays of probes that can be used for massive parallel fabrication of nanostructures by the DPN method.

We present a new database of our two-dimensional bulge-disk decompositions for 14,233 galaxies drawn from SDSS DR12 in order to examine the properties of bulges residing in the local universe (0.005 < z < 0.05). We performed decompositions in g and r bands by utilizing the galfit software. The bulge colors and bulge-tototal ratios are found to be sensitive to the details in the decomposition technique, and hence we hereby provide full details of our method. The g − r colors of bulges derived are almost constantly red regardless of bulge size except for the bulges in the low bulge-to-total ratio galaxies (B/T r 0.3). Bulges exhibit similar scaling relations to those followed by elliptical galaxies, but the bulges in galaxies with lower bulge-to-total ratios clearly show a gradually larger departure in slope from the elliptical galaxy sequence. The scatters around the scaling relations are also larger for the bulges in galaxies with lower bulge-to-total ratios. Both the departure in slopes and larger scatters are likely originated from the presence of young stars. The bulges in galaxies with low bulge-to-total ratios show signs of a frosting of young stars so substantial that their luminosity-weighted Balmer-line ages are as small as 1 Gyr in some cases. While bulges seem largely similar in optical properties to elliptical galaxies, they do show clear and systematic departures as a function of bulge-to-total ratio. The stellar properties and perhaps associated formation processes of bulges seem much more diverse than those of elliptical galaxies.

Endocrine disruptors (EDs) exert adverse effects on reproductive and immune function or neurological behavior. Bisphenol A (BPA), one of the environmental EDs, is widely used in the manufacture of plastics and epoxy resins. Studies reported that BPA affects reproductive organ growth and development. However, the potential adverse effects of BPA on neuronal development have not been fully explored. In this study, the potent harmful effects of BPA were investigated on the murine-derived multipotent neural progenitor cells (NPCs). Pretreatment of BPA significantly decreased proliferation of NPCs in a concentration-dependent manner. Moreover, at a high concentration (> 400 microM), BPA was cytotoxic to NPCs. However, the low concentrations of BPA, previously shown to exert estrogenic actions, did not affect the proliferation of NPCs. BPA altered the activation of extracellular signal-regulated kinases and c-Jun-N-Kinases in a different manner without affecting activities of p38 kinases. It was also found that reactive oxygen species (ROS) were elevated in NPCs exposed to high concentrations of BPA, indicating oxidative stress-related cytotoxicity. These data show adverse effects of BPA on the nervous system and potentially on neonatal brain development.

We present the KASI-Yonsei Deep Imaging Survey of Clusters (KYDISC) targeting 14 clusters at 0.015 z 0.144 using the Inamori Magellan Areal Camera and Spectrograph on the 6.5-meter Magellan Baade telescope and the MegaCam on the 3.6-meter Canada-France-Hawaii Telescope. We provide a catalog of cluster galaxies that lists magnitudes, redshifts, morphologies, bulge-to-total ratios, and local density. Based on the 1409 spectroscopically-confirmed cluster galaxies brighter than -19.8 in the r-band, we study galaxy morphology, color, and visual features generated by galaxy mergers. We see a clear trend between morphological content and cluster velocity dispersion, which was not presented by previous studies using local clusters. Passive spirals are preferentially found in a highly dense region (i.e., cluster center), indicating that they have gone through the environmental quenching. In deep images (µ r ∼ 27 mag arcsec −2 ), 20% of our sample show signatures of recent mergers, which is not expected from theoretical predictions and a low frequency of ongoing mergers in our sample (∼ 4%). Such a high fraction of recent mergers in the cluster environment supports a scenario that the merger events that made the features have preceded the galaxy accretion into the cluster environment. We conclude that mergers affect a cluster population mainly through the pre-processing on recently accreted galaxies.