The aim of this study was to compare the clinical and histopathological effects of tripeptide copper complex (TCC) and two different doses of laser application (helium-neon laser, 1 and 3 J cm(-2)) on wound healing with untreated control wounds. Experimental wounds were created on a total of 24 New Zealand white rabbits and topical TCC or laser was applied for 28 days. The wounds were observed daily, and planimetry was performed on days 7, 14, 21 and 28 to measure the unhealed wound area and percentage of total wound healing. Biopsies were taken weekly to evaluate the inflammatory response and the level of neovascularization. The median time for the first observable granulation tissue was shorter (P < 0.05) in the low and high dose laser groups than in the control group (3 and 2.66 vs. 4.5 days), but was not different between the TCC and control groups (4.16 vs. 4.5 days). Filling of the open wound to skin level with granulation tissue was faster (P < 0.05) in the TCC and high dose laser groups than in the control group (14 and 16 vs. 25 days), but was not different between the low dose laser and control groups (23 vs. 25 days). The average time for healing was shorter (P < 0.05) in the TCC and high dose laser groups (29.8 and 30.2 vs. 34.6 days), but was not different between the low dose laser and control groups (33.8 vs. 34.6 days). Histopathologically, wound healing was characterized by a decrease in the neutrophil counts and an increase in neovascularization. The TCC and high dose laser groups had greater neutrophil and vessel counts than in the control group, suggesting a more beneficial effect for wound healing.

We studied the effects of coating titanium implants with teicoplanin and clindamycin in 30 New Zealand White rabbits which were randomly assigned to three groups. The intramedullary canal of the left tibia of each rabbit was inoculated with 500 colony forming units of Staphylococcus aureus. Teicoplanin-coated implants were implanted into rabbits in group 1, clindamycin-coated implants into rabbits in group 2, and uncoated implants into those in group 3. All the rabbits were killed one week later. The implants were removed and cultured together with pieces of tibial bone and wound swabs. The rate of colonisation of the organisms in the three groups was compared. Organisms were cultured from no rabbits in group 1, one in group 2 but from all in group 3. There was no significant difference between groups 1 and 2 (p = 1.000). There were significant differences between groups 1 and 3 and groups 2 and 3 (p < 0.001). Significant protection against bacterial colonisation and infection was found with teicoplanin- and clindamycin-coated implants in this experimental model.

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SummaryThis study investigated the eff ects of topically applied oily homogenized and powder forms of bitter melon (Momordica charantia) (MC) and ointment formulation of gotu kola (Centella asiatica) (CA) extract and compared the results with untreated control and pure olive oil groups on wound healing in rabbits. A total of 30 New Zealand rabbits were divided into five equal groups (oily homogenized form of MC, powder form of MC, ointment of CA, control, pure olive oil). Full-thickness 5x5 cm skin wounds were created on the right mid-dorsum area and experimental groups were treated daily with the above mentioned extracts. Wounds were observed daily. Planimetry was performed for the unhealed wound area and the percentage of total wound healing on days 0, 7, 14, 21 and 28. Median time for the first observable granulation tissue was shorter in all experimental groups than in the control group (P<0.05). Filling of the open wound to skin level with granulation tissue was faster in the oily homogenized form of MC and ointment of titrated extract of CA groups (P<0.05). The average time for healing was shorter in the oily homogenized form of MC and ointment of titrated extract of CA groups than in other groups (P<0.05). The results demonstrate that topical application of the oily form of MC and ointment form of CA results in significant improvements on wound healing in rabbits. Keywords: Wound healing, Rabbit, Momordica charantia, Centella asiaticaTavşanlarda Kudret Narı (Momordica charantia) ve Gotu Kola (Centella asiatica) Ekstraktlarının Açık Yara İyileşmesi Üzerine Etkilerinin Karşılaştırılması ÖzetBu çalışmanın amacı; tavşanlarda açık yara iyileşmesinde topikal Momordica charantia'nın (MC) yağlı homojenize formu ve toz formları ile Centella asiatica (CA) ekstratının pomat formunun etkilerini araştırmak, saf zeytinyağı uygulanan ve sağaltım uygulanmayan kontrol grubu ile karşılaştırmaktı. Bu çalışmada kullanılan otuz adet Yeni Zelanda tavşanı 5 gruba ayrıldı: MC'nın yağlı homojenize formu, MC'nın toz formu, CA'nın titre edilmiş ekstratının pomat formu (Madécassol® pomat), kontrol ve saf zeytinyağı. Her bir tavşanda dorsal orta hattın sağ tarafında tam kalınlıkta deriyi kapsayan birer yara (5x5 cm) oluşturuldu ve yukarıda belirtilen ekstratlarla tedavi uygulandı. Yaralar günlük olarak izlendi ve 0, 7, 14, 21 ve 28. günlerde iyileşmemiş yara alanı ve total yara iyileşme yüzdesini ölçmek için planimetri uygulandı. İlk gözlenebilir granulasyon dokusu için ortalama zaman; tüm deney gruplarında kontrol grubundan daha kısa bulundu (P<0.05). Yara yatağının granulasyon dokusu ile deri düzeyine kadar dolması; MC yağlı homojenize formu ve CA titre edilmiş ekstratının pomat formu uygulanan gruplarda diğer gruplardan daha hızlı idi (P<0.05). Ortalama iyileşme zamanı, MC yağlı homojenize formu ve CA titre edilmiş ekstratının pomad formu uygulanan gruplarda, diğer gruplardan daha kısa idi (P<0.05). Bu çalışmada elde edilen sonuçlar; MC yağlı homojenize formu ve CA ekstratının pomad formunun topikal uygulamasının, tavşanlarda açık...

In this study, the effects of periosteal autograft and human amniotic membrane on tendon healing were compared. Forty-two New Zealand rabbits were divided into three groups. Flexor digitorum fibularis tendons were cut and repaired with a modified Kessler technique and circumferential sutures in all groups. Tendon repair alone was carried out in group 1, tendon repair and application of human amniotic membrane was done in group 2 and tendon repair and application of periosteal autograft was done in group 3. Biomechanical and histopathological examinations were done 2 and 6 weeks postoperatively. Biomechanical examination showed that group 3 was the strongest at weeks 2 and 6. Adhesion, inflammation and new bone formation showed no difference between groups at week 2. However, adhesion formation was found to be less in groups 2 and 3 than group 1 at week 6. The application of periosteal autograft might be useful in repair of tendon injuries.