The effects of human amniotic membrane and periosteal autograft on tendon healing: experimental study in rabbits

Abstract

In this study, the effects of periosteal autograft and human amniotic membrane on tendon healing were compared. Forty-two New Zealand rabbits were divided into three groups. Flexor digitorum fibularis tendons were cut and repaired with a modified Kessler technique and circumferential sutures in all groups. Tendon repair alone was carried out in group 1, tendon repair and application of human amniotic membrane was done in group 2 and tendon repair and application of periosteal autograft was done in group 3. Biomechanical and histopathological examinations were done 2 and 6 weeks postoperatively. Biomechanical examination showed that group 3 was the strongest at weeks 2 and 6. Adhesion, inflammation and new bone formation showed no difference between groups at week 2. However, adhesion formation was found to be less in groups 2 and 3 than group 1 at week 6. The application of periosteal autograft might be useful in repair of tendon injuries.

Cryopreserved Human Amniotic Membrane Injection for Plantar Fasciitis

“…Numerous preclinical and small clinical trials have evaluated their application for tendonitis, tendon repair, adhesion prevention, nerve repair, postoperative wounds, osteoarthritis, and spinal procedures. 10,18,20,21,23,29 They are also being used for several foot and ankle applications, such as diabetic foot ulcerations and other types of chronic wounds. 15,34,36 Although this study was randomized, controlled, and double-blind, there are several limitations that need to be addressed.…”

Cytokine expression and ultrastructural alterations in fresh-frozen, freeze-dried and γ-irradiated human amniotic membranes

“…Human amniotic membrane (HAM) has been used in a variety of surgical procedures. First employed in skin transplantation by (Davis 1910 ), HAM was subsequently found to be useful as a biological wound dressing for burns (Ramakrishnan and Jayaraman 1997 ; Branski et al 2008 ), acute (Tekin et al 2008 ) and chronic wounds (Gajiwala and Lobo 2003 ; Insausti et al 2010 ), and in the reconstruction of the dura mater (Tomita et al 2012 ; De Weerd et al 2013 ), oral cavity (Lawson 1985 ), vaginal vault (Ashworth et al 1986 ), tendons (Ozbölük et al 2010 ) and nerves (O’Neill et al 2009 ). HAM has also long been used in ophthalmic surgery, the earliest reported application being in 1940 when De Rötth used fetal membranes to correct symblepharon (De Rötth 1940 ).…”

Practice Patterns in Operative Flexor Tendon Laceration Repair: A 15-Year Analysis of Continuous Certification Data from the American Board of Plastic Surgery

“…Various agents have been used to reduce adhesions of the flexor tendon that may result after repair, including glycosaminoglycan gels, topical 5-fluorouracil, hyaluronic acid, platelet-rich plasma with fibrin matrix, mannose-6-phosphate, nonsteroidal anti-inflammatory drugs, amnion, and periosteum. [78][79][80][81][82][83][84] Botulinum toxin has also been used as an adjunct form of protection for tendon repair. 85,86 The current scope of evidence does not fully support the use of any particular agent; however, further research into their efficacy may help diminish tendon adhesion complications following flexor tendon repair.…”