The effects of topical tripeptide copper complex and helium‐neon laser on wound healing in rabbits

Abstract

The aim of this study was to compare the clinical and histopathological effects of tripeptide copper complex (TCC) and two different doses of laser application (helium-neon laser, 1 and 3 J cm(-2)) on wound healing with untreated control wounds. Experimental wounds were created on a total of 24 New Zealand white rabbits and topical TCC or laser was applied for 28 days. The wounds were observed daily, and planimetry was performed on days 7, 14, 21 and 28 to measure the unhealed wound area and percentage of total wound healing. Biopsies were taken weekly to evaluate the inflammatory response and the level of neovascularization. The median time for the first observable granulation tissue was shorter (P < 0.05) in the low and high dose laser groups than in the control group (3 and 2.66 vs. 4.5 days), but was not different between the TCC and control groups (4.16 vs. 4.5 days). Filling of the open wound to skin level with granulation tissue was faster (P < 0.05) in the TCC and high dose laser groups than in the control group (14 and 16 vs. 25 days), but was not different between the low dose laser and control groups (23 vs. 25 days). The average time for healing was shorter (P < 0.05) in the TCC and high dose laser groups (29.8 and 30.2 vs. 34.6 days), but was not different between the low dose laser and control groups (33.8 vs. 34.6 days). Histopathologically, wound healing was characterized by a decrease in the neutrophil counts and an increase in neovascularization. The TCC and high dose laser groups had greater neutrophil and vessel counts than in the control group, suggesting a more beneficial effect for wound healing.

Ankaferd Blood Stopper (ABS) ve Topikal Tripeptid Bakır Kompleksinin (TCC) Ratlarda Yara İyileşmesi Üzerine Etkisi: Deneysel Çalışma

“…TCC is a naturally occurring peptide-copper complex that has been shown to possess potentially interesting properties in the wound healing process in animal [21,37] and human models [20,38] . Observation of a positive effect by TCC on wound healing in the current study is consistent with other studies [23,24] which reported that Iamin, a TCC containing hydrogel, accelerates wound healing and wound contraction, and promotes epithelialization by creating a moist wound environment. The use of a hydrogel helps to produce more rapid healing by creating a moist environment which has superior features over a dry wound, such as prevention of tissue dehydration and cell death, accelerated angiogenesis, increased breakdown of dead tissue and fibrin, and potentiation of the interaction of growth factors with their target cells [39] .…”

“…Five-micrometer sections were stained with hematoxylin and eosin (H&E). Progressive decrease in neutrophil number and progressive increase in angiogenesis were selected for monitoring the healing process histopathologically as reported in previous studies [23,24] . In every skin section, an area just beneath the epidermis or crust formation was randomly selected.…”

“…For the histopathological investigation of wound healing several parameters, such as re-epithelialization, wound cellularity [23,24,40,41] , collagen deposition and blood vessel formation [23,24,42] have been used by different researchers. Decrease in neutrophil score and increase in vessel numbers were used to monitor the healing process in this study.…”

In vivo topical anti-inflammatory and wound healing activities of the fixed oil of Caryocar coriaceum Wittm. seeds

“…The measuring area was obtained comparing the amount of pixels inside perimeter and inside the known area using the mathematic expression: W a = (K a × N w )/N k , where W a = wound area, K a = known area, N w = number of pixels inside wound area and N k = number of pixels inside known area. Thus, the unhealed wound area and the percentage of wound contraction were calculated (Gul et al, 2008) and used for statistical analysis.…”

To evaluate the safety and efficiency of low level laser therapy (LLLT) in treating decubitus ulcers: a review

“…LLLT is a widely studied treatment option for several different types of acute and chronic medical problems, and has been studied mostly in different types of animals until now. Some of the studies using animals as the target subjects have found some correlation between the use of LLLT and decreased healing times of wounds when compared to the control groups 6,7 . As more evidence of the statistically proven benefits of LLLT in animal trials have surfaced, some researchers have begun to shift their view to whether this treatment may have any beneficial properties in human wound healing.…”