Our results suggest that alcohol extract of Tarantula cubensis accelerates epithelialisation and, thus, has beneficial effects on open wound healing in rats.

We studied the hemostatic and histopathological effects, and intra-abdominal adhesion scores of a new hemostatic agent, Ankaferd Blood Stopper (ABS), in an experimental liver injury model and compared it with regenerated oxidized cellulose. Thirty-six rats were randomly assigned to ABS, oxidized cellulose (Surgicel), and control groups (n=12, each). A wedge resection was performed on the left medial lobe of the liver. In the ABS group the liver surface was sprayed with ABS, whereas in the Surgicel group the liver was covered with double-layered oxidized cellulose. In the control group, saline solution was sprayed on the cut surface. The mean bleeding time was shorter in the ABS (23.08±6.99s) and Surgicel groups (47.91±8.21s) than in the control group (223.42±57.83s). No significant difference was found in the ABS and Surgicel groups in terms of preoperative and postoperative hematocrit (hct) values (P>0.05). Whereas there was no significant difference on day 7 (P>0.05), total adhesion score of ABS group was lower than both Surgicel (P<0.05) and control groups (P<0.01) on day 14. Liver sections from ABS group displayed more favorable histopathological changes when compared with Surgicel group on day 7 and day 14. All livers in the ABS group completed their regeneration process with minimal signs of inflammation. Our findings suggest that ABS is more effective than Surgicel and control groups in achieving hemostasis and in reducing blood loss. Apart from this, ABS causes more encouraging histopathological changes and better intra-abdominal adhesion scores in rat experimental liver trauma model.

In this study, the effects of Ankaferd Blood Stopper (ABS) and silver sulphadiazine (SSD) cream on burn wound healing were investigated in rats. A total of 24 outbred, male, Sprague-Dawley rats were randomly allocated to (1) ABS, (2) SSD, and (3) control groups. Bilateral burn wounds were created near the caudal border of the scapula. Wounds in each group were treated daily with sponges soaked in ABS solution, 1% SSD cream, or saline, respectively. On days 0, 7, 14, 21, and 28, unhealed wound area was measured and biopsy samples were taken for histopathological examination (except day 0). At the end of day 28, all rats in the ABS and SSD groups had complete coverage of the wounds with granulation tissue and epithelialization, whereas wounds in the control group were not completely epithelialized. On day 7, the mean unhealed wound areas and the mean percentages of wound contraction were not significantly different among the groups. However, the mean percentage of wound contraction in the ABS and SSD groups was significantly higher than in the control group on days 14, 21, and 28. Histopathologically, wound healing was characterized by a decrease in neutrophil counts and an increase in vessel counts. Our results suggest that ABS can be successfully used for burn wound healing besides SSD.

SummaryThis study investigated the eff ects of topically applied oily homogenized and powder forms of bitter melon (Momordica charantia) (MC) and ointment formulation of gotu kola (Centella asiatica) (CA) extract and compared the results with untreated control and pure olive oil groups on wound healing in rabbits. A total of 30 New Zealand rabbits were divided into five equal groups (oily homogenized form of MC, powder form of MC, ointment of CA, control, pure olive oil). Full-thickness 5x5 cm skin wounds were created on the right mid-dorsum area and experimental groups were treated daily with the above mentioned extracts. Wounds were observed daily. Planimetry was performed for the unhealed wound area and the percentage of total wound healing on days 0, 7, 14, 21 and 28. Median time for the first observable granulation tissue was shorter in all experimental groups than in the control group (P<0.05). Filling of the open wound to skin level with granulation tissue was faster in the oily homogenized form of MC and ointment of titrated extract of CA groups (P<0.05). The average time for healing was shorter in the oily homogenized form of MC and ointment of titrated extract of CA groups than in other groups (P<0.05). The results demonstrate that topical application of the oily form of MC and ointment form of CA results in significant improvements on wound healing in rabbits. Keywords: Wound healing, Rabbit, Momordica charantia, Centella asiaticaTavşanlarda Kudret Narı (Momordica charantia) ve Gotu Kola (Centella asiatica) Ekstraktlarının Açık Yara İyileşmesi Üzerine Etkilerinin Karşılaştırılması ÖzetBu çalışmanın amacı; tavşanlarda açık yara iyileşmesinde topikal Momordica charantia'nın (MC) yağlı homojenize formu ve toz formları ile Centella asiatica (CA) ekstratının pomat formunun etkilerini araştırmak, saf zeytinyağı uygulanan ve sağaltım uygulanmayan kontrol grubu ile karşılaştırmaktı. Bu çalışmada kullanılan otuz adet Yeni Zelanda tavşanı 5 gruba ayrıldı: MC'nın yağlı homojenize formu, MC'nın toz formu, CA'nın titre edilmiş ekstratının pomat formu (Madécassol® pomat), kontrol ve saf zeytinyağı. Her bir tavşanda dorsal orta hattın sağ tarafında tam kalınlıkta deriyi kapsayan birer yara (5x5 cm) oluşturuldu ve yukarıda belirtilen ekstratlarla tedavi uygulandı. Yaralar günlük olarak izlendi ve 0, 7, 14, 21 ve 28. günlerde iyileşmemiş yara alanı ve total yara iyileşme yüzdesini ölçmek için planimetri uygulandı. İlk gözlenebilir granulasyon dokusu için ortalama zaman; tüm deney gruplarında kontrol grubundan daha kısa bulundu (P<0.05). Yara yatağının granulasyon dokusu ile deri düzeyine kadar dolması; MC yağlı homojenize formu ve CA titre edilmiş ekstratının pomat formu uygulanan gruplarda diğer gruplardan daha hızlı idi (P<0.05). Ortalama iyileşme zamanı, MC yağlı homojenize formu ve CA titre edilmiş ekstratının pomad formu uygulanan gruplarda, diğer gruplardan daha kısa idi (P<0.05). Bu çalışmada elde edilen sonuçlar; MC yağlı homojenize formu ve CA ekstratının pomad formunun topikal uygulamasının, tavşanlarda açık...

We investigated the effects of Ankaferd Blood Stopper (ABS) and tripeptide copper complex (TCC) on wound healing in rats. A total of 24 outbred, male, Sprague-Dawley rats were randomly divided into (1) ABS, (2) TCC, and (3) control groups. Bilateral experimental wounds were created near the caudal border of the scapula. Each wound in the respective treatment group was treated daily with sponges soaked in ABS solution, topical TCC gel, or saline. On days 0, 7, 14 and 21, unhealed wound area was measured and biopsy samples were taken for histopathological analysis (except day 0). Median time for the first observable granulation tissue was not significantly different in the ABS, TCC, and control groups (5.8, 5.5, and 6.7 days, respectively) (P>0.05). Filling of the open wound with granulation tissue to skin level was significantly slower in the control group than in the ABS and TCC groups (18, 9, and 11 days, respectively). The mean unhealed wound area was significantly smaller and the mean percentage of total wound healing was significantly higher in the ABS-and TCC-treated wounds than in the control wounds on day 7, 14, and 21 (P<0.05); the average time for healing was also significantly shorter in the treatment groups than in the control group (17.4, 16.8 vs. 23.6 days, respectively) (P<0.05). Our results suggest that topical application of ABS and TCC have beneficial effects on wound healing. Keywords: Wound healing, Ankaferd Blood Stopper (ABS), Tripeptide copper complex (TCC), Skin, Rat Ankaferd Blood Stopper (ABS) ve Topikal Tripeptid Bakır Kompleksinin (TCC) Ratlarda Yara İyileşmesi Üzerine Etkisi:Deneysel Çalışma ÖzetBu çalışmada, Ankaferd Blood Stopper (ABS) ve tripeptid bakır kompleksinin (TCC) ratlarda yara iyileşmesi üzerine etkilerinin araştırılması amaçlanmıştır. Sprague-Dawley ırkı, dişi, 24 adet rat, rastgele şekilde 1) ABS, 2) TCC, ve 3) Kontrol olmak üzere 3 gruba ayrıldı. Skapulanın kaudal sınırına yakın sahada bilateral deneysel yaralar oluşturuldu. Her bir yara günlük olarak grubuna göre ABS solüsyon emdirilmiş sponjlar, topikal TCC jel ya da serum fizyolojik ile tedavi edildi. İyileşmemiş yara alanı 0, 7, 14 ve 21. günlerde ölçüldü ve histopatolojik analiz için biyopsi örnekleri alındı (0. gün haricinde). İlk gözlenebilir granulasyon dokusu oluşumu ortalama zamanı açısından ABS, TCC ve kontrol grupları arasında anlamlı istatistiki fark yoktu (sırasıyla 5.8, 5.5 ve 6.7 gün) (P>0.05). Açık yara sahasının deri düzeyine kadar granulasyon dokusu ile dolma süresi, kontrol grubunda ABS ve TCC grubundan anlamlı şekilde daha düşüktü (sırasıyla 18, 9 ve 11 gün). ABS ve TCC ile tedavi edilen yaraların 7, 14 ve 21. günlerde yapılan ölçümlerinde ortalama iyileşmemiş yara sahasının kontrol grubuna kıyasla anlamlı şekilde daha küçük ve ortalama total yara iyileşme yüzdesinin kontrol grubuna göre anlamlı şekilde daha büyük olduğu gözlendi (P<0.05); ortalama iyileşme zamanı da uygulama yapılan gruplarda kontrol grubundan anlamlı şekilde daha kısa (sırasıyla 17.4, 16.8 ve 23.6 gün) (P<0.05) olarak bulundu. Eld...