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The objective of the present investigation was to formulate and evaluate microencapsulated Glibenclamide produced by the emulsion – solvent evaporation method. Microparticles were prepared using Eudragit RLPO by emulsion solvent evaporation method and characterized for their micromeritic properties, encapsulation efficiency, particle size, drug loading, FTIR, DSC, SEM analysis. In vitro release studies were performed in phosphate buffer (pH 7.4). Stability studies were conducted as per ICH guidelines. The resulting microparticles obtained by solvent evaporation method were free flowing in nature. The mean particle size of microparticles ranges from 134.49 – 179.72 µm and encapsulation efficiency ranges from 92.30-98.32%. The infrared spectra and differential scanning calorimetry thermographs confirmed the stable character of Glibenclamide in the drug-loaded microparticles. Scanning electron microscopy revealed that the microparticles were spherical in nature. In vitro release studies revealed that the drug release was sustained up to 12 hrs. The release kinetics of Glibenclamide from optimized formulation followed zero-order and peppas mechanism. The mechanism of drug release from the microparticles was found to be non-Fickian type. Eudragit RLPO microparticles containing Glibenclamide could be prepared successfully by using an emulsion solvent evaporation technique, which will not only sustain the release of drug but also manage complicacy of the diabetes in a better manner.DOI: http://dx.doi.org/10.3329/icpj.v2i12.17016 International Current Pharmaceutical Journal, November 2013, 2(12): 196-201

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Plants are the natural treasure of antioxidants and antimicrobial agents. Present study was intended to evaluate the antioxidant and antimicrobial potential of Plumeria alba leaves hydroalcoholic extract (PALHE). Study involved the phytochemical screening, antioxidant, and antibacterial activity of PALHE. The PALHE was prepared using 95% ethanol as solvent through maceration method. The antioxidant activity involved determination of total phenolic content (TPC) and total flavonoid content (TFC). DPPH (1,1-Diphenyl2-picryl hydrazyl) radical scavenging assay was used for the determination of antioxidant activity of PALHE. The PALHE was investigated for its antibacterial activity against S. aureus and E. coli using well diffusion method. Various phytochemical screening tests were carried out and the results revealed the presence of carbohydrates, reducing sugar, mucilage, proteins, steroids, volatile oil, tannins, phenolic and flavonoids in the PALHE. Besides, moderate antioxidant activity was also revealed through the result of DPPH assay over the ethanolic leaves extract of the PALHE, where the IC50 was found to be 23.96 mcg/ml. Additionally, the TPC and TFC were found to be 71.04 mg (GAE/g of total phenol in terms of gallic acid equivalent) and 75.60 mg (RE/g of total flavonoid in terms of rutin equivalent) respectively.