Antioxidant and antibacterial response of hydroalcoholic extract of Plumeria alba leaves

Abstract

Plants are the natural treasure of antioxidants and antimicrobial agents. Present study was intended to evaluate the antioxidant and antimicrobial potential of Plumeria alba leaves hydroalcoholic extract (PALHE). Study involved the phytochemical screening, antioxidant, and antibacterial activity of PALHE. The PALHE was prepared using 95% ethanol as solvent through maceration method. The antioxidant activity involved determination of total phenolic content (TPC) and total flavonoid content (TFC). DPPH (1,1-Diphenyl2-picryl hydrazyl) radical scavenging assay was used for the determination of antioxidant activity of PALHE. The PALHE was investigated for its antibacterial activity against S. aureus and E. coli using well diffusion method. Various phytochemical screening tests were carried out and the results revealed the presence of carbohydrates, reducing sugar, mucilage, proteins, steroids, volatile oil, tannins, phenolic and flavonoids in the PALHE. Besides, moderate antioxidant activity was also revealed through the result of DPPH assay over the ethanolic leaves extract of the PALHE, where the IC50 was found to be 23.96 mcg/ml. Additionally, the TPC and TFC were found to be 71.04 mg (GAE/g of total phenol in terms of gallic acid equivalent) and 75.60 mg (RE/g of total flavonoid in terms of rutin equivalent) respectively.