The nutmeg (Myristica fragrans) flesh extract has a strong antioxidant activity. Therefore, M. fragrans flesh can be developed for functional drinks which are sources rich in antioxidants good for the prevention and treatment of diseases such as cancer and cardiovascular diseases. However, the tannins' content can cause a bitter and sour taste. Therefore, the tannins content should be reduced by the addition of egg white. The purpose of this study is to find out the comparison of antioxidant activity between a combination of M. fragrans flesh extract with various concentrations of egg white and to correlate its antioxidant activities with tannin, total flavonoid, and total phenolic contents. The antioxidant activities were conducted on M. fragrans flesh extract by using DPPH and ABTS radicals. Tannin, total flavonoid, and total phenolic contents from M. fragrans flesh extract were also tested. The M. fragrans flesh extracts without addition egg white have a strong antioxidant in scavenging the stable free radical ABTS (89.980±0.480 µg/mL) and intermediate antioxidant in scavenging the stable free radical DPPH (105.669±0.102 µg/mL). It is followed accordingly by tannin, total flavonoid, and total phenolic contents, namely 14.034±0.100 %w/w TAE, 26.929±0.129 %w/w QE, and 53.164±0.129 %w/w GAE, respectively. Correlation of tannin, total flavonoid, and total phenolic contents, which inhibited DPPH and ABTS radicals had R2 values of about 89.23-97.63%. It showed that antioxidant activity is strongly influenced by the tannin, total flavonoid, and total phenolic contents. Therefore, precipitation from the tannin-protein bond caused antioxidant activities were decreased.
This paper will identify some of the barriers and challenges in teaching English early in Indonesia. We believe that teaching English as a foreign language has many obstacles and challenges that must be raised and published so that all parties with interest in teaching foreign languages ??in Indonesia, especially in early childhood, will receive enlightenment. So, we have first received much input from various data sources that we access electronically. All of our data is related to the purpose of this study, and we analyze it with a descriptive qualitative design under the control of a phenomenological approach so that the results will be valid and reliable findings. Based on the discussion of the findings, we can conclude that many teachers are often hampered by a lack of enthusiasm, limited study schedules, teaching resources, inadequate materials, and excess students in class. On the other hand, students have shallow learning motivation and very few skills related to learning English as a foreigner. Therefore, this is an insight for many parties who work in succeeding in English teaching in Indonesia.
Antibiotics are chemicals produced by microorganisms that have ability to inhibit the growth or kill other microorganisms. Used of antibiotics in a self-medication is influenced by user knowledge. So that if the user knowledge is incorrect can cause errors in use. This research aims to determinate the level of knowledge and use of antibiotics on the community of Tomohon City. This research was an descriptive study tend to observational, with a cross-sectional approach to 262 respondents who met the inclusion criteria. The results to showed the level of knowledge of antibiotics by community in Tomohon City which are categorized as good (31%), moderate (21%) and less (48%); for the level of used antibiotics by community in Tomohon City which are categorized as good (39%), moderate (44%) and less (17%) and there is a direct correlation between knowledge and the used of antibiotics with significant value of 0.000 and correlation coefficient value of 0.524 which means if the proper knowledge then was done in proper use.Keywords: Antibiotics, self-medication, knowledge, use Abstrak: Antibiotik merupakan zat kimiawi yang dihasilkan oleh mikroorganisme yang mempunyai kemampuan untuk menghambat pertumbuhan atau membunuh mikroorganisme lainnya. Penggunaan antibiotik secara swamedikasi dipengaruhi oleh pengetahuan pengguna, sehingga apabila pengetahuan pengguna tidak tepat dapat menyebabkan kesalahan dalam penggunaan. Penelitian ini bertujuan untuk mengetahui tingkat pengetahuan antibiotik dan penggunaan antibiotik pada masyarakat di kota Tomohon. Penelitian ini merupakan penelitian deskriptif yang bersifat observasional dengan pendekatan cross-sectional terhadap 262 responden yang memenuhi kriteria inklusi. Hasil penelitian yang diperoleh yaitu tingkat pengetahuan antibiotik pada masyarakat di Kota Tomohon yang termasuk kategori baik (31%), cukup (21%) dan kurang (48%); untuk tingkat penggunaan antibiotik pada masyarakat di Kota Tomohon yang termasuk kategori baik (39%), cukup (44%) dan kurang (17%) serta terdapat hubungan yang searah antara pengetahuan dan penggunaan antibiotik dengan nilai signifikansi sebesar 0,000 dan nilai koefisien korelasi sebesar 0,524 yang berarti apabila pengetahuan yang dimiliki tepat maka penggunaan pun dilakukan secara tepat.Kata kunci: Antibiotik, swamedikasi, pengetahuan, penggunaan.
Irrational use of antibiotics occurs because of a lack of public knowledge about the use of antibiotics that a good and right. Good knowledge will change attitudes so that the actions taken become directed. This study aims to determine the effect of the level of knowledge and attitudes of the community towards the use of antibiotics at Kimia Farma Pharmacy 396 Tuminting, Manado city. This research is a descriptive study and data collection tool used is a questionnaire about antibiotic knowledge and attitudes towards antibiotic use to 290 respondents. Based on the research results obtained, it is known that the level of public knowledge about antibiotics is still classified as a poor with a percentage of 69% while public attitudes towards the use of antibiotics are quite sufficient with a percentage of 45% and there is an influence between the level of knowledge and attitudes towards antibiotic use with a significant value of 0,000.Key words : Antibiotics, Level of Knowledge, Attitude. ABSTRAK Penggunaan antibiotik yang tidak rasional terjadi karena kurangnya pengetahuan masyarakat tentang penggunaan antibiotik yang baik dan benar. Pengetahuan yang baik akan merubah sikap sehingga tindakan yang diambil menjadi terarah. Penelitian ini bertujuan untuk mengetahui pengaruh tingkat pengetahuan dan sikap masyarakat terhadap penggunaan antibiotik di Apotek Kimia Farma 396 Tuminting, kota Manado. Penelitian ini merupakan penelitian deskriptif dan alat pengumpulan data yang digunakan yaitu kuesioner tentang pengetahuan antibiotik dan sikap terhadap penggunaan antibiotik kepada 290 responden. Berdasarkan hasil penelitian yang diperoleh, diketahui bahwa tingkat pengetahuan masyarakat tentang antibiotik masih tergolong kurang dengan persentase 69% sementara sikap masyarakat terhadap penggunaan antibiotik tergolong cukup dengan persentase 45% dan terdapat pengaruh antara tingkat pengetahuan dan sikap terhadap penggunaan antibiotik dengan nilai signifikan sebesar 0,000.Kata kunci : Antibiotik, Tingkat Pengetahuan, Sikap.
Noni is a nutritious plant because it has several ingredients that are important for health. Antibacterial components in noni leaves include glycosides, acubins, saponins and flavonoids. The purpose of this study was to determine the fraction of the extract of noni leaf ethanol whether it has an antibacterial effect and know the class of compounds indentifed as having antibacterial activity after TLC-Bioautografi testing was carried out. The samples were extracted using maceration method with 96% ethanol solvent and fractionation using liquid-liquid fractionation method with methanol, ethyl acetat and n-hexane solvents. Antibacterial activity testing was carried out with concentration variants of 20%, 30% and 40% with agar diffusion method (Kirby and Bauer). The results showed that methanol fraction, ethyl acetat fraction and Noni hexane leaf fraction effectively inhibited Staphylococcus aureus and Escherichia coli bacteria. The largest fraction of inhibiting zone was the concentration of 40% methanol which was categorized as strong. TLC monitoring was carried out using the mobile phase of chloroform : n-hexane (2:1). Antibacterial activity testing carried out by contact bioautography method showed that there were spots on the TLC choromatogram, which produced inhibitory zones. Characteristics of blotches were performed with AlCl3 spotting and it was thought that the spots were flavonoids. Keywords : Noni, Antibacterial, Staphylococcus aureus, Escherichia coli, TLC-Bioautografi. ABSTRAK Mengkudu merupakan tanaman yang berkhasiat karena mempunyai beberapa kandungan senyawa yang penting bagi kesehatan tubuh. Komponen yang berkhasiat antibakteri dalam daun mengkudu antara lain adalah glikosida, acubin, saponin dan flavonoid. Tujuan dari penelitian ini adalah untuk mengetahui fraksi dari esktrak etanol daun mengkudu apakah memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT-Bioautografi. Sampel diekstraksi menggunakan metode maserasi dengan pelarut etanol 96% dan fraksinasi dengan metode fraksinasi cair-cair dengan pelarut metanol, etil asetat dan n-heksan. Pengujian aktivitas antibakteri dilakukan dengan varian konsentrasi 20%, 30% dan 40% dengan metode difusi agar (Kirby dan Bauer). Hasil penelitian menunjukan bahwa fraksi metanol, fraksi etil asetat dan fraksi n-heksan daun Mengkudu efektif menghambat bakteri staphylococcus aureus dan Escherichia coli. fraksi yang paling besar zona hambatnya yaitu fraksi metanol konsentrasi 30% yang dikategorikan kuat, dilakukan pemantauan KLT menggunakan fase gerak kloroform : n-heksan (2:1). Pengujian aktivitas antibakteri dilakukan dengan metode bioautografi kontak hasilnya menunjukkan terdapat bercak pada kromatogram KLT yang menghasilkan zona hambat. Karakteristik bercak dilakukan dengan penampak bercak AlCl3 dan diduga bahwa bercak tersebut Flavonoid. Kata kunci : Mengkudu, Antibakteri, Staphylococcus aureus, Escherichia coli , KLT-Bioautografi
White galangal rhizome (Alpinia galanga (L.) Willd's) scientifically proven has properties as antibacterial, antifungal, anticancer, antitumor, antioxidant and cytotoxic. In nano size, the contact surface area of the particle becomes larger which can increase the amount of active substance which is isolated more so that the antibacterial activity is stronger. This study aims to determine whether the white galangal extract nanoparticles have antibacterial activity against the bacteria Klebsiella pneumoniae and measure the inhibitory ability of white galangal extract nanoparticles against bacterial growth and compared with white galangal ethanol extract. White galangal was extracted using maceration method with ethanol solvent and nanoparticle formulation made of white galangal extract by ionic gelation method. The antibacterial activity test was carried out using agar diffusion method and observations were made 1x24 hours incubation period, with inhibition zones measured using a millimeter ruler. The results showed that the white galangal extract nanoparticles had antibacterial activity to inhibit the bacteria Klebsiella pneumoniae, with the results of inhibition zone measurements of 22.5 mm. Keywords : Antibacterial, Extraction, Klebsiella pneumoniae, Nanoparticles, White galangal. ABSTRAKRimpang lengkuas putih (Alpinia galanga (L.) Willd) memiliki khasiat yang sudah dibuktikan secara ilmiah sebagai antibakteri, antijamur, antikanker, antitumor, antioksidan dan sitotoksik. Dalam ukuran nano, luas kontak permukaan partikel menjadi lebih besar yang dapat meningkatkan jumlah zat aktif yang terisolasi lebih banyak sehingga aktivitas antibakteri semakin kuat. Penelitian ini bertujuan untuk mengetahui apakah nanopartikel ekstrak lengkuas putih memiliki aktivitas antibakteri terhadap bakteri Klebsiella pneumoniae serta mengukur kemampuan daya hambat nanopartikel ekstrak lengkuas putih terhadap pertumbuhan bakteri dan dibandingkan dengan ekstrak etanol lengkuas putih. Lengkuas putih diekstraksi menggunakan metode maserasi dengan pelarut etanol dan dibuat formulasi nanopartikel ekstrak lengkuas putih dengan metode gelasi ionik. Untuk pengujian aktivitas antibakteri dilakukan dengan metode difusi agar dan pengamatan dilakukan 1x24 jam masa inkubasi, dengan zona hambat diukur menggunakan penggaris millimeter. Hasil penelitian menunjukkan bahwa nanopartikel ekstrak lengkuas putih memiliki aktivitas antibakteri untuk menghambat bakteri Klebsiella pneumoniae, dengan hasil pengukuran zona hambat sebesar 22,5 mm. Kata Kunci : Lengkuas putih, Nanopartikel, Antibakteri, Ekstraksi, Klebsiella pneumoniae.
Mangosteen leaves have flavonoid compounds, tannins, and saponins that can be efficacious as antibacterial. The aim of this study was to determine the fraction of mangosteen leaves having an antibacterial effect and knowing the class of compounds identified as having antibacterial activity after TLC- Bioautography testing was carried out. The samples were extracted using 96% maceratarion method and fractioned using liquid-liquid fractionation method with methanol, n-hexane and ethyl acetate solvents, antibacterial activity using agar diffusion method (Kirby and Bauer) with 3 concetrations namely 10%, 20% and 30%. Thin Layer Chromatography (TLC) uses n-hexane and chloroform solvens. TLC-Bioautography uses contact bioautography methods. The resultd showed that mangosteen leaves in methanol fraction with a concentration of 30% had a very large inhibitory activity again Staphylococcus aureus and ethyl acetate fraction with a concentration of 30% had the gratest antibacterial activity against Escherichia coli. The results of the TLC- Bioautography study showed that the flavonoids compounds after spraying with AlCl3 and the mangosteen leaf Biosutography test had inhibitory zone activity against the bacteria Staphylococcus aureus dan Escherichia coli. Keywords: Mangosteen Leaves. Antibacterial, TLC Bioautography. ABSTRAK Daun manggis mempunyai senyawa flavonoid, tanin, dan saponin yang dapat berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui fraksi daun manggis memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT Bioautografi. Sampel diektrak dengan metode maserasi dengan pelarut 96% dan difraksinasi dengan metode fraksinasi cair-cair dengan pelarut metanol, n-heksan dan etil asetat, aktivitas antibakteri menggunakan metode difusi agar (Kirby and Bauer) dengan 3 kosentrasi yaitu 10%, 20% dan 30%. Kromatografi Lapis Tipis (KLT) menggunakan pelarut n-heksan dan klorofom. KLT-Bioautografi menggunakan metode bioautografi kontak. Hasil penelitian menunjukan daun manggis pada fraksi metanol dengan kosentrasi 30% memiliki aktivitas zona hambat ppaling besar terhadapat bakteri Staphylococcus aureus dan fraksi etil asetat dengan kosentrasi 30% memiliki aktivitas antibakteri paling besar terhadap Escherichia coli. Hasil penelitian KLT-Bioautografi menunjukan golongan senyawa flavonoid setelah disemprotkan dengan AlCl3 dan uji Bioautografi daun manggis memiliki aktivitas zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kata kunci : Daun Manggis, Antibakteri, KLT- Bioautografi.
The level of satisfaction achievement on the services provided to patients, both the quality of health services and the health care system, will be the first choice for patients to visit the hospital. The objective of this study was to determine inpatients satisfaction level in the internal diseases room on drug services at Pharmacy Installation Hospital of X Tahuna. This research was a descriptive research with crosssectional design. The research instrument was a form of a questionnaire of hope and reality which was adopted from Nangaro in 2019. The validity and reliability test of the questionnaire was conducted on 30 respondents and data analysis using index of total quality service. There are 115 people who have met the inclusion criteria, such as being willing to be a respondent, being able to read and write, aged ≥ 18 years old, and were currently undergoing treatment in the interna room and have received service at X Tahuna Hospital. Analysis of satisfaction data was using the Weighted Servqual model. The results showed an overall satisfaction index of -0.20, while the satisfaction index for each dimension was as follows: -0.29 for the tangibles dimension, -0.278 emphaty dimensions, -0.17 reliability dimensions, -0.274 dimensions responsiveness, and - 0.32 for the assurance dimension. The overall satisfaction index as well as each dimension shows a negative value, which means that the patient is not satisfied with the drug services at X Tahuna Hospital Pharmacy Installation. The Hospital must re-evaluate the number of human resources (pharmacist-patient ratio), the flow of drug services, as well as the completeness of the available facilities.
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