Correlation Analysis of Antioxidant Activities with Tannin, Total Flavonoid, and Total Phenolic Contents of Nutmeg (Myristica fragrans Houtt) Fruit Precipitated by Egg white

Abstract

The nutmeg (Myristica fragrans) flesh extract has a strong antioxidant activity. Therefore, M. fragrans flesh can be developed for functional drinks which are sources rich in antioxidants good for the prevention and treatment of diseases such as cancer and cardiovascular diseases. However, the tannins' content can cause a bitter and sour taste. Therefore, the tannins content should be reduced by the addition of egg white. The purpose of this study is to find out the comparison of antioxidant activity between a combination of M. fragrans flesh extract with various concentrations of egg white and to correlate its antioxidant activities with tannin, total flavonoid, and total phenolic contents. The antioxidant activities were conducted on M. fragrans flesh extract by using DPPH and ABTS radicals. Tannin, total flavonoid, and total phenolic contents from M. fragrans flesh extract were also tested. The M. fragrans flesh extracts without addition egg white have a strong antioxidant in scavenging the stable free radical ABTS (89.980±0.480 µg/mL) and intermediate antioxidant in scavenging the stable free radical DPPH (105.669±0.102 µg/mL). It is followed accordingly by tannin, total flavonoid, and total phenolic contents, namely 14.034±0.100 %w/w TAE, 26.929±0.129 %w/w QE, and 53.164±0.129 %w/w GAE, respectively. Correlation of tannin, total flavonoid, and total phenolic contents, which inhibited DPPH and ABTS radicals had R2 values of about 89.23-97.63%. It showed that antioxidant activity is strongly influenced by the tannin, total flavonoid, and total phenolic contents. Therefore, precipitation from the tannin-protein bond caused antioxidant activities were decreased.

Determination of Total Phenolic Content and Total Flavonoid Content of Nutmeg Flesh (Myristica Fragrans Houtt) Ethanol Extract from South Aceh Province

“…al. [21] nutmeg extract has antioxidant activity that is classified as strong with the antioxidant activity value obtained of 105.669 μg/mL. The presence of phenolic compounds and flavonoids in the ethanol extract of nutmeg flesh shows that there are many compounds that are polar in nature [33].…”

“…al. [21] method. The extract of nutmeg flesh weighed 20 mg and dissolved in ethanol to 10 mL, with tripplecate experiment.…”

“…The total flavonoid content was stated as mg equivalent quercetin mg/mL extract. The total of flavonoid content in sample is stated as gram quercetin equivalent (QE)/100 gram sample (%w/w QE) [21].…”

Uji Aktivitas Antimikroba Ekstrak Etanol Daging Buah dan Daun Pala (Myristica fragrans)

“…Ekstrak daging buah mengandung flavonoid dan Terpenoid [19]. Aktivitas antioksidan ekstrak daging M. fragrans dipengaruhi oleh kandungan tanin, flavonoid total, dan total fenol sekitar 89,23-97,63% [25]. Ekstrak alkaloid merupakan molekul yang memiliki komposisi aromatik dan mengandung gugus karbonil.…”

Comparison of different methods for evaluating the antioxidant activity of instant Torbangun (Coleus amboinicus L.) drinks

“…DPPH (2,2-diphenyl-1-picrylhydrazil), ABTS (2,2-azinobis-(3ethylbenzothiazoline-6-sulfonic acid), and FRAP (Ferric Reducing Antioxidant Power) are common IOP Publishing doi:10.1088/1755-1315/1324/1/012124 2 methods for evaluating antioxidant activity of plant extracts due to simple, rapid, low cost, and applicable to conduct at room temperature. Those methods are based on single electron transfer and the antioxidant activity is measured by monitoring color changing of reaction [7].…”