The aim of this experiment was to study the effect of BCG vaccination on phagocyte activity and ROI secretion in cat peritoneum macrophages which infected with M.tuberculosis. The experiment used twenty four healthy cats. The animals were divided in 2 groups, 12 cats in each group. Group I were vaccinated with BCG, group II were control group which unvaccinated. BCG vaccination was done twice in two weeks interval. Two days after vaccination each cat was infected by 10 5 cfu M.tuberculosis intraperitoneally. The activity of macrophages were measured at 1 st , 2 nd , 12 th , and 24 th , after infection using in vitro latex bead phagocyte and NBT reduction assay. Three cats were used to measure the macrophage activity in each period, using triplicate sample for each cat. The results of the experiment showed that the phagocyte activity and ROI secretion increased significantly in vaccination group (P<0.01) compared with the control group, and these activities reached to the plateau level at 2 weeks after infection. Although these enhanced activities were gradually diminished thereafter, higher levels of these activities were consistently observed until the end of experiment compared with control group. BCG vaccination increased the cellular immunity especially phagocyte and ROI secretion activities of peritoneual macrophages in cat infected with M.tuberculosis
Leptospirosis is a zoonotic disease of global concern, and is caused by pathogenic serovar Leptospira interrogans. Canine Leptospirososis is widespread worldwide, dogs can act as incidental hosts or maintenance hosts for various serovars. The purpose of this research was to identify leptospire serovars that infect healthy and suspected leptospirosis dogs in Yogyakarta. A total of 56 dogs (36 healthy dogs and 20 suspect leptospirosis dogs) sera were taken from cephalica vein as much as 3 ml. Sera were examined for leptospirosis with Microscopic Aglutination Test (MAT) which conducted at the Research Center for Veterinary Science, Bogor. Microscopic Aglutination Test carried out on various Leptospire serovar, namely: Ichterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Australis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, and Tarrasovi. The results showed that Celledoni serovars infected 25% of healthy dogs and 5% of suspect leptospirosis dogs, Javanica serovar infected 19% of healthy dogs, Bataviae serovars infected 15% of suspect leptospirosis dogs, Grippotyphosa serovar infected 11% of healthy dogs, Tarrasovi serovar infected 10% of suspect leptospirosis dogs, serovars Cynopteri infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, serovar Pyrogenes infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, and serovar Rachmati infects 5% of suspect leptospirosis dogs. Seven healthy dogs (19%) and 2 suspect leptospirosis dogs (10%) were infected with more than 2 leptospire serovars. From the results of this study it can be concluded that Celledoni serovar of Leptospira interrogans infection causes subclinical leptospirosis, while Bataviae serovar infection causes clinical leptospirosis in dogs in Yogyakarta.
The aim of this study was to determine the prevalence and risk factors Dirofilariaimmitis (D. immitis) infection in dogs slaughtered in Yogyakarta. A total of 151 dogs that were slaughtered from May – November 2013 were examined their heart in order to determine the presence of D. immitis infection. Blood samples were tested using Modified Knott’s Technique for microfilariae examination. The results showed that based on the heart and blood examination the prevalence of D. immitis infection was 14.6 % and 7.9 %, respectively. The risk factors for D. immitis infection were the age and origin of the dog.
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