Present investigation was carried out on 28 repeat breeding cross bred cows during their post-partum period in their second to seventh lactation from different villages of Satara district. Serum was separated from collected blood samples and analyzed for certain biochemical parameters viz. protein, albumin, globulin, cholesterol, calcium, and phosphorus and blood urea nitrogen. These serum biochemical constituents were compared with serum levels in normal cyclical and repeat breeding cattle reported by various researchers.

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Parthenogenesis is a form of asexual reproduction found in females, where growth and development of embryos occurs without fertilization by a male. Parthenogenesis occurs naturally in aphids, Daphnia, rotifers, nematodes and some other invertebrates but can also be induced efficiently in mammalian oocytes by providing appropriate stimuli in-vitro. Recently, parthenogenesis has attracted wide attention because of the role of activated oocytes in the field of research that have been described such as intra cytoplasmic sperm injection, cloning by nuclear transfer, somatic cell cloning, investigating culture conditions etc. & potential for deriving pluripotent stem cell lines and their differentiation into various cell lines that can be utilized for various tissue engineering applications. The parthenogenetically activated oocytes possess maternal genome and can developed in to either haploid, diploid or polyploidy embryos with the help of it we can analyze the possible role of all the genes involved in imprinting processes as well as the role the paternal genome plays during early embryo development by comparing them with fertilized embryos. Several methods are able to induce parthenogenetic activation through the elevation of cytoplasmic free calcium in oocytes. But one common, universal method or activation agents has not been developed for all species because the process is highly specific for each species. Therefore, activation step for each species need to be optimized accordingly. This review describes the general method of activation of mammalian oocytes and their genomic imprinting analysis.

The effect of dietary supplementation of crushed flaxseed and soybean oil on ovarian function in postpartum Jersey crossbred cows was studied. Total 30 postpartum normally calved cows from 2nd to 4th lactation were randomly divided into three equal groups. Cows from group T1 and T2 were fed with dietary supplementation of crushed flaxseed @ 15% of DM requirement from day 25 to day 70 postpartum and with soybean oil @ 3% of DM requirement of animal from day 50 to day 70 postpartum in addition to routine feed respectively, whereas cows from Group T3 was considered as control, supplemented with routine feed. Follicular dynamic study with the help of USG was carried out at 24 hrs interval before the first injection of GnRH of Ovsynch protocol and during Ovsynch protocol (Day 0 to Day 9) of synchronization, which was implemented from day 60 postpartum in all the groups. It was observed that mean number of class I follicles was significantly increased in T2 group, whereas the mean number of class II and total number of follicles were significantly increased in T1 and T2 groups on day 0. During the synchronization period the mean number of class I, class II, total numbers of follicles and preovulatory follicles size were significantly larger in T1 and T2 groups. It was concluded that dietary supplementation of crushed flaxseed and soybean oil helps in increasing class I, class II, total number of follicles and size of preovulatory follicle in crossbred cows.

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Asymptomatic infection with Rotavirus C (RVC) at 20 percent detection rate was recorded in pigs from India. Sequencing of the VP6, VP7, and NSP4 genes of RVC strains identified I7/ I10, G1 and E5 genotypes, respectively. Full genome sequencing of one of these strains revealed additional P1, R1, C1, M3, A1, N5, T5, H1 genotypes, of VP4, VP1, VP2, VP3, NSP1, NSP2, NSP3, NSP5 genes, respectively. Detection of porcine RVC strains at two different locations in India and at different time points strongly suggests their continuous circulation in the pig population through asymptomatic infections.

The study was conducted on six porcine males and thirty six semen ejaculates for its fertility assessment. The average length of the left and right testicle of the boars measured by ultrasonography was 9.47 ± 0.73 cm and 9.09 ± 0.65 cm, respectively. The sperm concentration/ml increased significantly as testicular diameter increased in size. The average length of right and left of boar testicle measured by Vernier caliper was 10.4 ± 0.57 cm and average width was 4.3 ± 0.14 cm. The average volume of boar semen was 115.00 ± 11.83 ml with milky colour. Thick consistency was observed in 83.3% semen samples whereas 16.6 % semen samples were having thin consistency. Out of 36 semen ejaculates, 16.6 % semen samples had density of DD where as 83.3 % had a density of DDD. The Mass activity, live percentage, percentage of morphologically abnormal spermatozoa and total sperm concentration in boar semen were 3 ± 0, 75.41 ± 2.07 %, 0 %, and 523 ± 60.07 million/ml, respectively. Mean percentage of hypo osmotic swelling test (HOS-Test) of semen found in the present study was 73.47 ± 2.26. The average time for reduction of resazurin dye from blue to violet was 1.805 ± 0.163 and from violet to pink was 9.944 ± 0.890. None of the sample change colour from pink to white. In the present study 36 ejaculates of boar semen were subjected for TVC of bacteria. In order to differentiate the bacterial species contaminating semen raw semen was placed on different agar plates. The species isolated with higher frequency in boar semen were of Staphylococcus species-83.3% (30 samples) followed by E.coli-63.8% (23 samples). Mean TVC obtained in this study was 45.13 × 10 3 .

An experiment on ultrasonographic studies of follicular activities in clomiphene citrate treated anoestrus cows was conducted. Ultrasound scanning was performed to record the follicular activities on alternate day throughout one estrous cycle for control group and from drug administration to induction of estrus in treatment group. The postpartum induced estrus (treatment group) and regular estrus (control group) differed highly significantly (p is less than 0.01) with respect to number of follicles involved in ovulatory wave (6.67 ± 0.21 Vs 5.00 ± 0.45) and significantly (pis less than 0.05) with respect to growth rate (1.82 ± 0.34 Vs 1.04 ± 0.94 mm/day) and increase in diameter of dominant follicle (7.58 ± 0.72 Vs 10.2 ± 0.47 mm). Maximum diameter of ovulatory follicle in induced estrus was greater than regular estrus (16.12 ± 0.56 Vs15.18 ± 0.47 mm), however, it differed non-significantly