Present investigation was carried out on 28 repeat breeding cross bred cows during their post-partum period in their second to seventh lactation from different villages of Satara district. Serum was separated from collected blood samples and analyzed for certain biochemical parameters viz. protein, albumin, globulin, cholesterol, calcium, and phosphorus and blood urea nitrogen. These serum biochemical constituents were compared with serum levels in normal cyclical and repeat breeding cattle reported by various researchers.

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Parthenogenesis is a form of asexual reproduction found in females, where growth and development of embryos occurs without fertilization by a male. Parthenogenesis occurs naturally in aphids, Daphnia, rotifers, nematodes and some other invertebrates but can also be induced efficiently in mammalian oocytes by providing appropriate stimuli in-vitro. Recently, parthenogenesis has attracted wide attention because of the role of activated oocytes in the field of research that have been described such as intra cytoplasmic sperm injection, cloning by nuclear transfer, somatic cell cloning, investigating culture conditions etc. & potential for deriving pluripotent stem cell lines and their differentiation into various cell lines that can be utilized for various tissue engineering applications. The parthenogenetically activated oocytes possess maternal genome and can developed in to either haploid, diploid or polyploidy embryos with the help of it we can analyze the possible role of all the genes involved in imprinting processes as well as the role the paternal genome plays during early embryo development by comparing them with fertilized embryos. Several methods are able to induce parthenogenetic activation through the elevation of cytoplasmic free calcium in oocytes. But one common, universal method or activation agents has not been developed for all species because the process is highly specific for each species. Therefore, activation step for each species need to be optimized accordingly. This review describes the general method of activation of mammalian oocytes and their genomic imprinting analysis.

The effect of dietary supplementation of crushed flaxseed and soybean oil on ovarian function in postpartum Jersey crossbred cows was studied. Total 30 postpartum normally calved cows from 2nd to 4th lactation were randomly divided into three equal groups. Cows from group T1 and T2 were fed with dietary supplementation of crushed flaxseed @ 15% of DM requirement from day 25 to day 70 postpartum and with soybean oil @ 3% of DM requirement of animal from day 50 to day 70 postpartum in addition to routine feed respectively, whereas cows from Group T3 was considered as control, supplemented with routine feed. Follicular dynamic study with the help of USG was carried out at 24 hrs interval before the first injection of GnRH of Ovsynch protocol and during Ovsynch protocol (Day 0 to Day 9) of synchronization, which was implemented from day 60 postpartum in all the groups. It was observed that mean number of class I follicles was significantly increased in T2 group, whereas the mean number of class II and total number of follicles were significantly increased in T1 and T2 groups on day 0. During the synchronization period the mean number of class I, class II, total numbers of follicles and preovulatory follicles size were significantly larger in T1 and T2 groups. It was concluded that dietary supplementation of crushed flaxseed and soybean oil helps in increasing class I, class II, total number of follicles and size of preovulatory follicle in crossbred cows.

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