Rotavirus C infections in asymptomatic piglets in India, 2009-2013: genotyping and phylogenetic analysis of all genomic segments

Joshi, M. V.; Arya, Shalu A.; Shinde, Manohar; Ingle, V.C.; Birade, H. S.; Gopalkrishna, Varanasi

doi:10.1007/s00705-022-05607-3

Cited by 2 publications

(3 citation statements)

References 39 publications

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“…Rotaviruses are endemic in pig populations and varying detection rates have been reported [ 11 , 12 , 13 , 14 ]. Infected pigs can have clinical or subclinical symptoms with neonatal and suckling piglets worst affected.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Analyses of Rotavirus A, B and C Detected on a Porcine Farm in South Africa

Strydom,

Segone,

Coertze

et al. 2024

Viruses

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Rotaviruses (RVs) are known to infect various avian and mammalian hosts, including swine. The most common RVs associated with infection in pigs are A, B, C and H (RVA-C; RVH). In this study we analysed rotavirus strains circulating on a porcine farm in the Western Cape province of South Africa over a two-year period. Whole genomes were determined by sequencing using Illumina MiSeq without prior genome amplification. Fifteen RVA genomes, one RVB genome and a partial RVC genome were identified. Phylogenetic analyses of the RVA data suggested circulation of one dominant strain (G5-P[6]/P[13]/P[23]-I5-R1-C1-M1-A8-N1-T7-E1-H1), typical of South African porcine strains, although not closely related to previously detected South African porcine strains. Reassortment with three VP4-encoding P genotypes was detected. The study also reports the first complete RVB genome (G14-P[5]-I13-R4-C4-M4-A10-T4-E4-H7) from Africa. The partial RVC (G6-P[5]-IX-R1-C1-MX-A9-N6-T6-EX-H7) strain also grouped with porcine strains. The study shows the continued circulation of an RVA strain, with a high reassortment rate of the VP4-encoding segment, on the porcine farm. Furthermore, incidents of RVB and RVC on this farm emphasize the complex epidemiology of rotavirus in pigs.

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Section: Introductionmentioning

confidence: 99%

Phylogenetic Analyses of Rotavirus A, B and C Detected on a Porcine Farm in South Africa

Strydom,

Segone,

Coertze

et al. 2024

Viruses

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“…Porcine RVA was first identified in diarrheic piglets from Australia in 1975, followed by RVB and RVC in piglets from the United Kingdom in 1980s and the United States in Pathogens 2023, 12, 1091 2 of 15 1980, respectively [5][6][7]. Compared to RVB and RVC, RVA is considered to be the most predominant rotavirus due to its high detection rate in different pig herds [4,8].…”

Section: Introductionmentioning

confidence: 99%

“…Compared to RVB and RVC, RVA is considered to be the most predominant rotavirus due to its high detection rate in different pig herds [ 4 , 8 ]. Porcine RVC has been identified in many countries around the world [ 9 , 10 , 11 , 12 , 13 , 14 ]. After 2009, the prevalence of RVC infection in animals increased from 10% to 25% [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a Quadruplex RT-qPCR for the Detection of Porcine Rotaviruses and the Phylogenetic Analysis of Porcine RVH in China

Shi,

Zhou,

Feng

et al. 2023

Pathogens

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Rotavirus A species (RVA), RVB, RVC, and RVH are four species of rotaviruses (RVs) that are prevalent in pig herds, and co-infections occur frequently. In this study, a quadruplex real-time quantitative RT-PCR (RT-qPCR) for the simultaneous detection of four porcine RVs was developed by designing specific primers and probes based on the VP6 gene of RVA, RVB, RVC, and RVH, respectively. The method showed high specificity and could only detect RVA, RVB, RVC, and RVH, without cross-reaction with other porcine viruses; showed excellent sensitivity, with a limit of detection (LOD) of 1.5 copies/µL for each virus; showed good repeatability, with intra-assay coefficients of variation (CVs) of 0.15–1.14% and inter-assay CVs of 0.07–0.96%. A total of 1447 clinical fecal samples from Guangxi province in China were tested using the developed quadruplex RT-qPCR. The results showed that RVA (42.71%, 618/1447), RVB (26.95%, 390/1447), RVC (42.92%, 621/1447), and RVH (13.68%, 198/1447) were simultaneously circulating in the pig herds, and the co-infection rate of different species of rotaviruses was found to be up to 44.01% (579/1447). The clinical samples were also detected using one previously reported method, and the coincidence rate of the detection results using two methods was more than 99.65%. The phylogenetic tree based on the VP6 gene sequences of RVH revealed that the porcine RVH strains from Guangxi province belonged to the genotype I5, which was closely related to Japanese and Vietnamese strains. In summary, an efficient, sensitive, and accurate method for the detection and differentiation of RVA, RVB, RVC, and RVH was developed and applied to investigate the prevalence of porcine RVs in Guangxi province, China. This study is the first to report the prevalence of porcine RVH in China.

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Rotavirus C infections in asymptomatic piglets in India, 2009-2013: genotyping and phylogenetic analysis of all genomic segments

Cited by 2 publications

References 39 publications

Phylogenetic Analyses of Rotavirus A, B and C Detected on a Porcine Farm in South Africa

Phylogenetic Analyses of Rotavirus A, B and C Detected on a Porcine Farm in South Africa

Development of a Quadruplex RT-qPCR for the Detection of Porcine Rotaviruses and the Phylogenetic Analysis of Porcine RVH in China

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