Effect of Dietary Supplementation of Crushed Flaxseed and Soybean Oil on Ovarian Functions in Postpartum Jersey Crossbred Cows

Abstract

The effect of dietary supplementation of crushed flaxseed and soybean oil on ovarian function in postpartum Jersey crossbred cows was studied. Total 30 postpartum normally calved cows from 2nd to 4th lactation were randomly divided into three equal groups. Cows from group T1 and T2 were fed with dietary supplementation of crushed flaxseed @ 15% of DM requirement from day 25 to day 70 postpartum and with soybean oil @ 3% of DM requirement of animal from day 50 to day 70 postpartum in addition to routine feed respectively, whereas cows from Group T3 was considered as control, supplemented with routine feed. Follicular dynamic study with the help of USG was carried out at 24 hrs interval before the first injection of GnRH of Ovsynch protocol and during Ovsynch protocol (Day 0 to Day 9) of synchronization, which was implemented from day 60 postpartum in all the groups. It was observed that mean number of class I follicles was significantly increased in T2 group, whereas the mean number of class II and total number of follicles were significantly increased in T1 and T2 groups on day 0. During the synchronization period the mean number of class I, class II, total numbers of follicles and preovulatory follicles size were significantly larger in T1 and T2 groups. It was concluded that dietary supplementation of crushed flaxseed and soybean oil helps in increasing class I, class II, total number of follicles and size of preovulatory follicle in crossbred cows.

Effect of dietary supplementation of crushed soybean and flaxseed on ovarian response in postpartum crossbred dairy cows

“…Supplementation of soybean was effective in increasing the number of medium sized follicles compared to a saturated fat or a highly unsaturated fat (fish oil) supplement and this was associated with higher serum insulin and increased granulose cell proliferation (Poretsky and Kalin, 1987) [13] . In the present study, the significant increase in class II and total number of follicle on day 0 in supplemented group is in accordance with Deshmukh et al (2017) [2] who reported significant increase in class II and total number of follicles after supplementation of soybean oil and flaxseed in post partum cows. The mean total number of class I follicles showed non significant difference between the supplemented and non supplemented groups.…”

“…The mean bearing different superscripts in a row, differs significantly. The present finding regarding mean growth rate (mm/day) of ovulatory follicle, mean day of emergence of new follicle was in accordance with Deshmukh et al (2017) [2] who reported a significant difference in growth rate and the mean day of emergence of new follicle with supplementation of soybean oil and crushed flaxseed as compared to no fat supplementation group. Similarly, the mean day of emergence of new follicle after injection of first GnRH was in close agreement with Twagiramunga et al (1995) [19] .…”

“…Whereas the mean total number of Class III follicles were at par T1 and T2 groups (Table 2) The mean bearing different superscripts in a row, differs significantly. These findings regarding the significant increase in Class I, class II (medium) follicles and total number of follicles were in close accordance with Deshmukh et al (2017) [2] with feeding soybean oil and crushed flaxseed, Ghasemzadeh et al (2011) [5] with feeding fish oil and soybean oil. Ponter et al (2006) [12] recorded increase in small size follicles whereas medium size follicles was in accordance with others (Thomas et al 1997;Robinson et al 2002;Kassa et al 2002 and Gulliver et al 2012) [18,14,9,7] who reported increase in medium size follicles with feeding different source of linolenic and linoleic acid.…”

“…The mean total number of class I follicles showed non significant difference between the supplemented and non supplemented groups. On the contrary Deshmukh et al (2017) [2] reported a significant increase in class I follicles in the supplemented group.…”

“…The animals were provided clean drinking water for 24 hours. The roasted crushed soybean was supplemented @10% of DM (300 g/100 kg of body weight/animal/day; as per Dirandeh et al, 2013 [3] as a supplement over and above the feed which was routinely fed, on a dry matter basis from the day 0 Postpartum (PP) today 50 and thereafter, supplementation of crushed flaxseed @ 15% DM (300 g/100 kg of body weight/animal/day as per Deshmukh et al, (2017) [2] was started from day 51 PP and was continued up to 85 days postpartum. Whereas Group II control (T2) animals were fed routinely with regular diet/feed.…”