The study was conducted on six porcine males and thirty six semen ejaculates for its fertility assessment. The average length of the left and right testicle of the boars measured by ultrasonography was 9.47 ± 0.73 cm and 9.09 ± 0.65 cm, respectively. The sperm concentration/ml increased significantly as testicular diameter increased in size. The average length of right and left of boar testicle measured by Vernier caliper was 10.4 ± 0.57 cm and average width was 4.3 ± 0.14 cm. The average volume of boar semen was 115.00 ± 11.83 ml with milky colour. Thick consistency was observed in 83.3% semen samples whereas 16.6 % semen samples were having thin consistency. Out of 36 semen ejaculates, 16.6 % semen samples had density of DD where as 83.3 % had a density of DDD. The Mass activity, live percentage, percentage of morphologically abnormal spermatozoa and total sperm concentration in boar semen were 3 ± 0, 75.41 ± 2.07 %, 0 %, and 523 ± 60.07 million/ml, respectively. Mean percentage of hypo osmotic swelling test (HOS-Test) of semen found in the present study was 73.47 ± 2.26. The average time for reduction of resazurin dye from blue to violet was 1.805 ± 0.163 and from violet to pink was 9.944 ± 0.890. None of the sample change colour from pink to white. In the present study 36 ejaculates of boar semen were subjected for TVC of bacteria. In order to differentiate the bacterial species contaminating semen raw semen was placed on different agar plates. The species isolated with higher frequency in boar semen were of Staphylococcus species-83.3% (30 samples) followed by E.coli-63.8% (23 samples). Mean TVC obtained in this study was 45.13 × 10 3 .
Total 104 parous goats of Sangamneri (62) and Osmanabadi (42) breeds aged 2 -4 years were used in this study. All goats were randomly distributed in six treatment groups. Group wise applications of hormones used for the estrus synchronization and controlled breeding protocols including both breeds of goat were as under: Group I-Two dosages of Inj. PGF 2 α; Group II -Intravaginal sponges + Inj. eCG i.m. on the day of sponge removal; Group III -Intra-vaginal sponges + Inj. PGF 2 α i.m. 24 h prior to sponge removal + Inj. eCG i.m. on the day of sponge removal; Group IVIntra-vaginal sponges + Inj. GnRH i.m. 24 h prior to sponge removal; Group V -Intra vaginal sponges + Inj. GnRH (i.m.) on the day of sponge removal; Group VI Intra-vaginal sponges + Inj. eCG (i.m.) 24 h before sponge removal. The percentage of goats exhibiting estrus from sponge withdrawal or from the end of treatment for treatment Groups I, II, III, IV and V in Sangamneri goats were 66. 67, 100, 100, 100, 91.67 % and in Osmanabadi goats as 69.23, 100, 100, 100, 57.14 %, respectively. One Sangamneri goat in Group IV exhibited estrus for seven days after sponge removal. Estrus response was 100 % for both Sangamneri and Osmanabadi breeds of goats subjected to estrus synchronization belonging to treatment Groups II, III and IV. The overall percentage of goats exhibiting estrus following different synchronization protocols were 89.28 % and 83.33 %, in Sangamneri and Osmanabadi goats, respectively. The mean time of onset of estrus (h) from progestagen withdrawal for five different treatment groups in Sangamneri goats were 25.20 ± 5.50, 46.91 ± 2.53, 27.33 ± 2.26, 41.33 ± 3.53, and 48.55 ± 4.16 h, respectively and in Osmanabadi goats as 44.4 ± 3.36, 24.0 ± 6.28, 26.75 ± 4.12, 46.84 ± 3.88, and 48.0 ± 9.09 h, respectively. The overall mean interval of onset of estrus in Sangamneri and Osmanabadi goats were 37.20 ± 2.31 h and 37.08 ± 5.72 h, respectively. The mean estrus duration (h) for five different treatment groups following estrus synchronization in Sangamneri goats were 54.60 ± 4.60, 76.36 ± 7.81, 56.67 ± 5.92, 44.00 ± 7.48, and 69.27 ± 8.76 h, respectively and for Osmanabadi goats as 44.00 ± 3.46, 113.14 ± 6.65, 53.50 ± 6.63, 50.86 ± 6.69, and 51.0 ± 15.78 h, respectively. The overall mean duration of estrus in Sangamneri and Osmanabadi goats were recorded as 61.08 ± 3.51 h and 62.34 ± 5.38 h, respectively. There was variation in estrus interval among different treatment groups of both the breeds. The conception rates in Sangamneri goats were 40. 00, 72.73, 66.67, 88.89, and 72.72 %, Open Access Research ArticleIJAVST -An Open Access Journal (ISSN 2320-3595) International Journal of Advanced Veterinary Science and Technology 309respectively and in Osmanabadi goats in five treatment groups as 44.44, 57.14, 37.50, 71.43, 100.00 %, respectively. The overall pregnancy rate in Sangamneri goats in this study was 68.00 %, whereas for Osmanabadi goats it was 57.14 %. Out of 34 pregnant Sangamneri goats, 21 goats' kidded and 32 kids were delivered. In...
Background: In vitro embryo production in buffaloes has gained much importance in this current scenario due to ever increasing population and high demand of milk and meat. Slaughter house derived bubaline ovaries are a cheap and abundant source of cumulus oocyte complexes.Methods: Oocytes from the buffalo ovarian follicles were recovered by aspiration technique as it facilitates quick recovery. Total 155 ovaries were used in the present study. Surface follicles were measured using vernier calliper and categorized into three groups viz. less than 3 mm, 3-5 mm and greater than 5 mm based on follicular diameter and oocytes were processed for IVM, IVF and IVC using conventional non sorted semen.Result: Overall percentage of small, medium and large follicles in the ovaries were recorded as 16.29 ± 0.94%, 8.14±0.60%, 5.35 ± 0.76%, respectively. Overall recovery rate of COCs was 38%. The percentage of these oocytes were 16.74% (A), 15.25% (B), 25.26% (C), 18.33% (D) and 29.87% (E) respectively. Maturation rate of oocytes were 81.96 ± 2.70%. Fertilization rate was 74.98 ± 3.87%, Cleavage rate % was 40.84±2.51% and Blastocyst percentage was 21.57±1.75% respectively. Application of in vitro embryo production technique using slaughter house ovaries can salvage the genetic potential of bubaline species.
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