Background: Carvedilol is a commonly used drug in hypertension, congestive heart failure in diabetics. It has moderate calcium channel blocking property in addition to α1 and non selective β antagonistic activity. Though some studies bring forth the beneficial effects of Carvedilol in cardiovascular comorbidities in diabetes, there is no consensus on its effects on glycaemic levels. Aims:To evaluate the effect of oral Carvedilol administration for 5 days on blood glucose levels in normal albino rats through Oral Glucose Tolerance Test. Material and Methods:Twelve adult albino rats of either sex weighing between 150 -200 g were selected from central animal facility and randomly divided into 2 groups -Control [Distilled water (1ml/rat orally)] and Test (0.8mg/kg body weight orally) and the respective drugs were administered over 5 days. Following overnight fasting, on the fifth day 1 hour after the last dose of the respective drug, OGTT was performed. The CBG (Capillary Blood Glucose) levels were measured at 0 min, glucose (2g/ kg body weight) dissolved in water was administered to all the rats orally. The blood sample from tail vein (obtained by tail snipping) at 60 and 150 minutes were analysed for CBG levels using a standardized glucometer.Statistical Analysis: Data was presented as Mean ± SEM. One way ANOVA, independent samples t-test, non-parametric tests, percentages and cross tabs were used in the analysis of data within the same group and between different groups when required.Results: Carvedilol group showed higher CBG levels at all time intervals of OGTT as compared to the Control group i.e., 0, 60 and 150 minutes, the highest being (103.8±5.029 )mg/dl at 60 minutes and was statistically significant. Carvedilol group however showed lesser inter-interval variation compared to the Control group at the same time intervals respectively but was statistically insignificant. Conclusions:Carvedilol has hyperglycaemic potential when given orally for 5 days in normal albino rats. Though it may be beneficial in diabetics for various comorbid conditions, the glycaemic control may worsen during its use in subjects with prediabetes, diabetes, high risk diabetes.

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Background: The objective was to evaluate the analgesic activity of irbesartan in albino mice. Methods: Swiss albino mice weighing 25-30 g of either sex were selected for the study. Six animals were allocated to each experimental group. The control group received normal saline (25 ml/kg, p.o.), standard group received pentazocine (10mg/kg, intraperitonial [i.p.]) and test group received irbesartan (20 mg/kg, p.o.). The above drugs were administered 1 hr prior to the experiments. In case of visceral pain model 0.6% acetic acid was given i.p. 30 mins prior to the experiment to induce writhing, in thermal pain model pretreated mice were placed on Eddy's Hotplate maintained at 55°C and in mechanical stimulus pain model an artery clip was clamped at the base of the tail of pretreated mice. Decrease in total number of writhes in acetic acid induced writhing model and delay in reaction time in both Eddy's hot plate and Tail clip method denoted analgesic activity respectively. Results: The test drug signifi cantly decreased the total number of writhes in acetic acid induced writhing model in mice. The percentage inhibition of writhing was signifi cant which was 84.35% in the standard group and 59.24% in the test group. The test drug signifi cantly delayed the reaction time in both Eddy's hot plate and tail clip method when compared to control group and standard group. Percentage increase in latency period when compared to standard drug was signifi cant and measured 73.11% and 64.31% at 60 min in both Eddy's hot plate and tail clip method, respectively. Conclusion: Irbesartan exhibits analgesic activity in albino mice.

Background: Oringaoleifera is a widely used plant with high medicinal value, well known for its pharmacological actions and is used in various conditions. It has been reported to have many biological properties like anti-inflammatory, antimicrobial, antispasmodic, antitumour including antidiabetic activity.Methods: The study was carried out in Wistar albino rats with body weight 150-250gms. Diabetes was induced by injecting Streptozotocin intraperitoneally- dose 55 mg/kg BW. Animals were divided into 5 groups with 6 animals in each group. First group (Control) was given 2% gum acacia. Other 4 groups were induced diabetes by giving Streptozotocin. Diabetic control group received gum acacia (0.5 ml), Standard group received Glibenclamide (0.5mg/kg BW), Test group received Moringaoleifera extract (300mg/kg) and Test+ Standard group receiving combination of Moringaoleifera and glibenclamide at half the above doses. All drugs were given orally for 28 days and blood glucose levels analyzed using Glucometer on Day 0 before drug and on D1, D3, D7, D14, D21, and D28. Data were statistically analyzed by ANOVA and Tukey‘s Post Hoc test.Results: Hypoglycemia produced by Moringaoleifera extract was significant (p<0.001) when compared to diabetic control group from day 7 to day 28. The percent reduction of blood glucose level was 52.9% as compared to Glibenclamide group 61.3%. The combination group also showed significant hypoglycemic activity the percentage reduction being 56.44%.Conclusions: Thus, Moringaoleifera decreased blood glucose level efficaciously as compared to diabetic control group and similar to standard group at p<0.001.

Background: Analgesics selectively relieve pain by acting either on central or peripheral pain pathways. Recently, studies have shown accumulating evidence to implicate N-methyl-d-aspartate receptors (NMDARs) mediation in central and peripheral sensitization and visceral pain leading to the possibility that NMDAR antagonists may be useful in the treatment of pain. Aims and Objectives: (1) To evaluate analgesic activity of Pioglitazone (PIO) in mice. (2) To compare the analgesic activity of PIO with the standard drugs tramadol and aspirin, in mice. Materials and Methods: Albino mice were divided into four groups, containing six animals (n = 6) in each group (control, standard, and test group). Group-I: Control received saline solution 2 ml/kg orally, Group-II: Standard 1 received tramadol at a dose of 10 mg/ kg intraperitoneal, Group-III: Standard 2 received aspirin at a dose of 300 mg/kg orally, and Group-IV: Test received PIO at a dose of 20 mg/kg orally. PIO and normal saline were administered 30 min before, whereas the tramadol and aspirin were administered 15 min before writhing and tail clip methods. The decrease in number of writhes and the delay in reaction time in tail clip method denoted the analgesic activity. Results: PIO decreased the number of writhes and delayed the reaction time in tail clip method considerably when compared with control, but less when compared with standard drugs. Conclusion: PIO exhibits analgesic activity in both chemical and mechanical pain models in albino mice.

Background: Pioglitazone (PIO), a Peroxisome Proliferator Activated Receptor γ (PPAR-γ) agonist, is an oral anti-diabetic agent belonging to the group of thiazolidinediones-TZDs used for the treatment of diabetes mellitus type 2 in monotherapy and in combination with a sulfonylurea, metformin, or insulin.Methods: All animals were allowed to acclimatize with laboratory conditions at least two weeks before starting the experiment and they were maintained under the same condition throughout the experiment. They were given food and water ad libitum. The experiments were performed as per the Committee for the Purpose of Control and Supervision on Experiments on Animals (CPCSEA) guidelines. The animals were subjected to experimentation between 0900-1600 hours in noise free atmosphere with ambient temperature 23-300ºC.Results: There was no significant reduction in the within group comparisons of the basal and final scores in locomotor activity.Conclusions: The standard and test groups failed to produce any significant reduction in locomotor activity in the intergroup comparison as well as compared to normal control.