How to cite this article:Oliveira FP, Oliveira BGRB, Santana RF, Silva BP, Candido JSC. Nursing interventions and outcomes classifi cations in patients with wounds: cross-mapping. Rev Gaúcha Enferm. 2016 jun;37 (2) ABSTRACTObjective: Cross-mapping of terms referring to nursing interventions and outcomes in the medical records of patients with wounds compared to nursing interventions and nursing outcomes classifi cations. Method: An observational retrospective study based on cross-mapping. The sample consisted of 81 outpatients treated in a university hospital in Rio de Janeiro. Data was collected from medical records between May and July of 2013. The analysis was done in four steps: search for phrases used to describe interventions and outcomes; setting the frequency of occurrence of phrases; grouping synonyms; comparison of terms with taxonomies. Results: 13 interventions were mapped and the most frequent was "Care with wounds" (47.23%). Also, 6 results were mapped and the priority was "Wound healing: secondary intention" (45%). Conclusions:The use of cross-mapping of terms referring to interventions and nursing outcomes in patients with wounds suggested additions to the taxonomies for adaptation to outpatient care.

P. aeruginosa was present in 75% of tested chronic wound samples, and the same clones persisted for more than 1 month. In addition, most bacteria contained virulence genes that were associated with high potential to establish infection. The use of silver in chronic wounds may be associated with multidrug resistance in P. aeruginosa; therefore, it is important to avoid colonization by these bacteria.

Compared with those colonized by MSSA, chronic wounds colonized by MRSA did not display a strong correlation with the presence of a greater number of clinical signs of infection.

Wnt/β-catenin has been described as crucial for dorsal-ventral and antero-posterior patterning, playing multiple roles at different stages of development. Cholesterol-rich membrane microdomains (CRMMs), cholesterol- and sphingolipid-enriched domains of the plasma membrane, are known as platforms for signaling pathways. Although we have demonstrated the importance of the CRMMs for head development, how they participate in prechordal plate formation and embryo axis patterning remains an open question. Moreover, the participation of the CRMMs in the Wnt/β-catenin signaling pathway activity in vivo is unclear, particularly during embryonic development. In this study, we demonstrated that CRMMs disruption by methyl-beta-cyclodextrin (MβCD) potentiates the activation of the Wnt/β-catenin signaling pathway in vitro and in vivo during embryonic development, causing head defects by expanding the Wnt expression domain. Furthermore, we also found that the action of CRMMs depends on the microenvironmental context because it also works in conjunction with dkk1, when dkk1 is overexpressed. Thus, we propose CRMMs as a further mechanism of prechordal plate protection against the Wnt signals secreted by posterolateral cells, complementing the action of secreted antagonists.

RESUMO.-A utilização da transgenia com a proteína fluorescente verde (GFP) como marcador de células de origem fetal nas placentas de clones bovinos servirá de modelo inédito para estudo morfofisiológico e imunológico da interação materno-fetal, visto que possibilitará o seu mapeamento, diferenciando as células fetais das maternas. TalCaracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos The transgenic application of green fluorescent protein (GFP) as fetal cell marker on cattle cloned placenta could provide an exclusive model for studying the morphologic and immunologic maternal-fetal interactions, providing information about its mapping, distinguishing the fetal from maternal cells. This model will have direct application, mainly because these animals present problems during its development. With this model's support, we intend to verify the substances transport between mother and fetus during endocytosis, through the immunolocalization of protein named caveolae. For these, we used 06 cloned bovine and 30 cattle samples of artificial insemination (AI) with 90 days of pregnancy, which had been their development interrupted by humanitarian slaughter of the recipient and recovery of the pregnant uterus. We collected the placentome and the chorion. A part of the samples was cut and fixed, by immersion, on a solution containing 4% of parafomaldehyde or 10% of formaldehyde on a sodium phosphate buffer (PBS), at 0,1M pH 7.4, Zamboni solution (4% of paraformaldehyde, 15% of picric acid, on sodium phosphate buffer 0,1M pH 7.4), metacarn (60% of metanol, 30% of chloroform, and 10% glacial acetic acid), for morphologic and immunohistochemistry verification for caveolinas proteins -1 and -2 (CAV -1 and CAV-2). The caveolins -1 were found in fetal and maternal villi, but its strongest staining was observed in the endometrial stroma. The caveolins -2 had positive staining in trophoblast and chorioallantoic membrane, and specifically in giant trophoblastic binucleated cell. Therefore the results were compared between cloned cattle and from AI or natural mating, for assisting on detection of the reason of many placental alterations, embryonic losses, spontaneous abortion, post-natal mortality and large offspring syndrome on laboratory-manipulated animals. The result suggests that the proteins caveolins -1 and -2 (CAV-1 and CAV-2) are part of the caveolae composition and important structures related to the molecule transfer to the fetus, nourish it through endocytosis and pinocytosis.INDEX TERMS: Caveolin-1 and -2 proteins, caveolae, placentation, immunolocalization, cattle.

RESUMO:Objetivo: realizar o mapeamento cruzado dos termos identificados nos prontuários de pacientes com feridas de acordo com a Classificação de Diagnósticos de Enfermagem da NANDA Internacional (I) Método: pesquisa observacional retrospectiva, de mapeamento cruzado. Amostra composta por 81 prontuários e 405 evoluções de enfermagem de pacientes acompanhados em um ambulatório especializado no tratamento de feridas. Pesquisa desenvolvida em 2013, em duas etapas: mapeamento dos termos descritivos de diagnósticos de enfermagem para o cruzamento com a NANDA-I e, análise de concordância entre especialistas. Resultados: foram mapeados 309 termos descritivos correspondentes a 19 diagnósticos da NANDA-I (3,7±0,2), presentes em sete domínios da terminologia. Os mais frequentes foram Integridade da pele prejudicada (20,4%) e Risco de integridade da pele prejudicada (16,1%). Conclusão: o mapeamento cruzado permitiu identificar os principais diagnósticos de enfermagem em pacientes com feridas, indicando a possibilidade de elaboração de um protocolo clínico, que contribuirá para uma prática avançada em enfermagem. Palavras-chave: Úlcera; cicatrização de feridas; processos de enfermagem; cuidados de enfermagem. ABSTRACT:Objective: cross-mapping of terms identified in wound patient medical records, by NANDA International (I) Classification nursing diagnosis. Method: this retrospective observational study, using cross-mapping in a sample of 81 records and 405 nursing progress reports on patients treated at a specialized wound clinic, was conducted in 2013 in two steps: descriptive terms in nursing diagnoses were mapped to the intersection with NANDA-I and related expert agreement was analyzed. Results: 309 descriptive terms corresponding to 19 NANDA-I diagnoses (3.7  0.2), in seven areas of terminology, were mapped. The most common were Impaired skin integrity (20.4%) and Risk of impaired skin integrity (16.1%). Conclusion: cross-mapping identified the main nursing diagnoses in wound patients, indicating that it is possible to draft a clinical protocol to contribute to advanced nursing practice.

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Objetivo: Analisar evidências científicas sobre as apresentações farmacêuticas e os efeitos antimicrobianos dofator de crescimento epidérmico recombinante humano em feridas. Método: Revisão integrativa de literatura,nas bases de dados contidas no portal Biblioteca Virtual em Saúde e no Portal de Periódicos da Coordenaçãode Aperfeiçoamento de Pessoal de Nível Superior, com os termos úlcera varicosa, úlcera cutânea, úlcera daperna, infecção dos ferimentos e fator de crescimento epidérmico. Foram incluídos estudos experimentais ouquase-experimentais que abordassem como tema central avaliações da efetividade do fator de crescimentoepidérmico no reparo tecidual de feridas. Foram utilizadas as recomendações CONSORT como indicador dequalidade metodológica dos artigos. Resultados: Foram incluídos sete estudos, avaliados a partir das categorias:apresentações farmacêuticas de fator de crescimento epidérmico recombinante humano e seus efeitos no processode reparo tecidual de lesões e emprego de terapia antimicrobiana como coadjuvante no tratamento de feridascom fator de crescimento epidérmico recombinante humano. Os estudos apontaram para o uso coadjuvante defator de crescimento epidérmico com antimicrobianos. Conclusão: Os estudos avaliados não apontaram para apresença de atividades antimicrobianas do fator de crescimento epidérmico recombinante.Palavras-chave: Enfermagem; Fator de crescimento epidérmico; Úlcera varicosa; Úlcera da perna; Infecção dos ferimentos. ABSTRACTObjective: To analyze scientific evidence about pharmaceutical forms and antimicrobial effects of recombinanthuman epidermal growth factor in wounds. Method: Integrative review, in databases contained on the websiteVirtual Health Library and Journals of Higher Education Personnel Improvement Coordination, with key wordsvaricose ulcer, skin ulcer, leg ulcer, wound infection and epidermal growth factor. Experimental or quasiexperimental researchs about assessments related with the effectiveness of the epidermal growth factor inwound healing were included. CONSORT recommendations were used to evaluate the methodological quality ofstudies. Results: We included seven studies evaluated from the categories: pharmaceutical forms of recombinanthuman epidermal growth factor and its effects on wound healing process and employment antimicrobial therapyas an adjunct in the treatment of wounds with recombinant human epidermal growth factor. Studies indicated thatepidermal growth factor should be used with others antimicrobial agents to fight wound infections. Conclusion:Studies have not indicated the presence of antimicrobial activity in recombinant epidermal growth factor.Keywords: Nursing; Epidermal growth factor; Venous ulcer; Leg ulcer; Wound infection.