Prevalence, Antimicrobial Susceptibility, and Clonal Diversity of Pseudomonas aeruginosa in Chronic Wounds

Oliveira, Fernanda Pessanha de; Pires, Bruna Maiara Ferreira Barreto; Silva, Keila de Cássia Ferreira de Almeida; Carvalho, Bernadete Teixeira Ferreira; Teixeira, Lenise Arneiro; Paula, Geraldo Renato de; Oliveira, Beatriz Guitton Renaud Baptista de

doi:10.1097/won.0000000000000373

Cited by 22 publications

(23 citation statements)

References 27 publications

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“…First, diabetic patients can have higher levels of serum lactate due to diabetic ketoacidosis, and secondly, lactate is associated with wound bed colonization by bacteria which produce it as a byproduct 86 – 88 . > 80% of chronic wounds are colonized by P. aeruginosa 89 while 90% of chronic leg ulcers are colonized by S. aureus 78 , 90 – 93 which displays a cCCR phenotype 94 , 95 . Not surprisingly, these bacteria are often co-isolated 55 , 85 , 96 .…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa reverse diauxie is a multidimensional, optimized, resource utilization strategy

McGill

Yung

Hunt

et al. 2021

Sci Rep

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Pseudomonas aeruginosa is a globally-distributed bacterium often found in medical infections. The opportunistic pathogen uses a different, carbon catabolite repression (CCR) strategy than many, model microorganisms. It does not utilize a classic diauxie phenotype, nor does it follow common systems biology assumptions including preferential consumption of glucose with an ‘overflow’ metabolism. Despite these contradictions, P. aeruginosa is competitive in many, disparate environments underscoring knowledge gaps in microbial ecology and systems biology. Physiological, omics, and in silico analyses were used to quantify the P. aeruginosa CCR strategy known as ‘reverse diauxie’. An ecological basis of reverse diauxie was identified using a genome-scale, metabolic model interrogated with in vitro omics data. Reverse diauxie preference for lower energy, nonfermentable carbon sources, such as acetate or succinate over glucose, was predicted using a multidimensional strategy which minimized resource investment into central metabolism while completely oxidizing substrates. Application of a common, in silico optimization criterion, which maximizes growth rate, did not predict the reverse diauxie phenotypes. This study quantifies P. aeruginosa metabolic strategies foundational to its wide distribution and virulence including its potentially, mutualistic interactions with microorganisms found commonly in the environment and in medical infections.

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Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa reverse diauxie is a multidimensional, optimized, resource utilization strategy

McGill

Yung

Hunt

et al. 2021

Sci Rep

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“…PA infection is a common occurrence in chronic wounds [ 71 ] and is more fatal than other bacterial infections if left undetected or inappropriately treated [ 72 ]. In this study, we show that fluorescence imaging can rapidly detect PA in chronic wounds.…”

Section: Clinical Implications and Conclusionmentioning

confidence: 99%

Rapid Diagnosis of Pseudomonas aeruginosa in Wounds with Point-Of-Care Fluorescence Imaing

2021

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Pseudomonas aeruginosa (PA) is a common bacterial pathogen in chronic wounds known for its propensity to form biofilms and evade conventional treatment methods. Early detection of PA in wounds is critical to the mitigation of more severe wound outcomes. Point-of-care bacterial fluorescence imaging illuminates wounds with safe, violet light, triggering the production of cyan fluorescence from PA. A prospective single blind clinical study was conducted to determine the positive predictive value (PPV) of cyan fluorescence for the detection of PA in wounds. Bacterial fluorescence using the MolecuLight i:X imaging device revealed cyan fluorescence signal in 28 chronic wounds, including venous leg ulcers, surgical wounds, diabetic foot ulcers and other wound types. To correlate the cyan signal to the presence of PA, wound regions positive for cyan fluorescence were sampled via curettage. A semi-quantitative culture analysis of curettage samples confirmed the presence of PA in 26/28 wounds, resulting in a PPV of 92.9%. The bacterial load of PA from cyan-positive regions ranged from light to heavy. Less than 20% of wounds that were positive for PA exhibited the classic symptoms of PA infection. These findings suggest that cyan detected on fluorescence images can be used to reliably predict bacteria, specifically PA at the point-of-care.

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“…The identification of MRSA strains among patients from this study who were under ambulatory follow‐up care at a health unit located within a large hospital complex indicates a need to implement precautionary measures of contact in outpatient clinics to minimise the risks of cross‐contamination by multidrug‐resistant microorganisms. The results of previous studies indicated the non‐occurrence of this type of contamination in the units where this study was performed . However, periodic reassessments are warranted because selection pressure to generate multidrug‐resistant microorganisms increases proportionately with time and as the use of new commercially available antimicrobials increases.…”

Section: Discussionmentioning

confidence: 83%

Epidermal growth factor vs platelet‐rich plasma: Activity against chronic wound microbiota

Oliveira

Teixeira

et al. 2019

International Wound Journal

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The objective was to evaluate Staphylococcus aureus and Pseudomonas aeruginosa colonisation of wounds treated with recombinant epidermal growth factor (EGF) and platelet‐rich plasma (PRP); to analyse the susceptibility profiles of S. aureus and P. aeruginosa isolates from wounds treated with EGF and PRP; and to describe the presence of infection in EGF‐treated and PRP‐treated wounds. Experimental study was performed using clinical specimens collected with swabs. Patients were treated with PRP and EGF in the outpatient clinic of a university hospital. Forty‐three isolates were obtained from 31 patients, 41.9% (13/31) of whom had been treated with EGF and 58.0% (18/31) with PRP. Ten of the 43 isolates were identified as S. aureus, 60.0% (6/10) of which were isolated from PRP‐treated wounds. Among the 33 P. aeruginosa isolates, 66.6% (22/33) were isolated from PRP‐treated wounds. Regarding antimicrobial susceptibility, only one strain isolated from an EGF‐treated wound was identified as methicillin‐resistant S. aureus (MRSA). Among the P. aeruginosa isolates, one obtained from a patient treated with EGF was multidrug‐resistant. Patients treated with EGF had no infections during the follow‐up period, and there was a significant difference between the 1st and 12th week in wound infection improvement in patients treated with PRP (P = .0078).

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Prevalence, Antimicrobial Susceptibility, and Clonal Diversity of Pseudomonas aeruginosa in Chronic Wounds

Cited by 22 publications

References 27 publications

Pseudomonas aeruginosa reverse diauxie is a multidimensional, optimized, resource utilization strategy

Pseudomonas aeruginosa reverse diauxie is a multidimensional, optimized, resource utilization strategy

Rapid Diagnosis of Pseudomonas aeruginosa in Wounds with Point-Of-Care Fluorescence Imaing

Epidermal growth factor vs platelet‐rich plasma: Activity against chronic wound microbiota

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