Monitoring and Molecular Characterization of Staphylococcus aureus Isolated from Chronic Wounds

Pires, Bruna Maiara Ferreira Barreto; Oliveira, Fernanda Pessanha de; Oliveira, Beatriz Guitton Renaud Baptista de; Fuly, Patrí­cia dos Santos Claro; Ferreira-Carvalho, Bernadete Teixeira; Paula, Geraldo Renato de; Teixeira, Lenise Arneiro

doi:10.1097/01.asw.0000540069.99416.a6

Cited by 10 publications

(8 citation statements)

References 30 publications

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“…In a study carried out in the same scenario, Staphylococcus aureus was detected in 71.4% (25/35) of the wounds in samples collected by swab, 2 patients had Methicillin-resistant Staphylococcus aureus (MRSA); however, according to the clinical criteria, 8 (22.9%) patients presented signs of infection in their wounds. Wounds colonized with MRSA did not show a strong correlation with the presence of a greater number of clinical signs of infection 20 . Most of the wounds are colonized by bacteria, while only a few have clinically relevant wound infection characteristics 21 .…”

Section: Discussionmentioning

confidence: 82%

Construct Validity and Reliability of the Leg Ulcer Measurement Tool Scale Adapted for Brazil

Silveira

Oliveira

Souza

et al. 2022

Texto contexto - enferm.

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Objective: to validate the Portuguese version of the Leg Ulcer Measurement Tool regarding construct and reliability. Method: this is a methodological research study. Data collection was conducted between January and July 2019. A total of 105 participants were included for construct validation and internal consistency and, of these, 50 were selected for intra-observer stability. For the analysis of construct validity, factor analysis was performed; for internal consistency, Cronbach's alpha coefficient was calculated, and for stability, the intraclass correlation coefficient. Results: A total of 12items were confirmed for the Brazilian reality, meeting all the theoretical requirements of the factor analysis model. As for the internal consistency (reliability) analysis, the Cronbach's alpha coefficient values (α=0.711) showed that the set of items that make up the scale measures the same characteristics and presents internal consistency. In the global stability analysis (ICC=0.823), the Leg Ulcer Measurement Tool test and retest scores presented good agreement, showing that the adapted scale is stable. Conclusion: the Leg Ulcer Measurement Tool scale adapted to the Portuguese language contains 12 items, with scores varying from 0 to 4 and, thus, produces a score from 0 to 48 points. It presents construct validity analyzed by means of factor analysis and reliability in terms of internal consistency and stability.

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Section: Discussionmentioning

confidence: 82%

Construct Validity and Reliability of the Leg Ulcer Measurement Tool Scale Adapted for Brazil

Silveira

Oliveira

Souza

et al. 2022

Texto contexto - enferm.

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show abstract

“…The identification of MRSA strains among patients from this study who were under ambulatory follow‐up care at a health unit located within a large hospital complex indicates a need to implement precautionary measures of contact in outpatient clinics to minimise the risks of cross‐contamination by multidrug‐resistant microorganisms. The results of previous studies indicated the non‐occurrence of this type of contamination in the units where this study was performed . However, periodic reassessments are warranted because selection pressure to generate multidrug‐resistant microorganisms increases proportionately with time and as the use of new commercially available antimicrobials increases.…”

Section: Discussionmentioning

confidence: 83%

Epidermal growth factor vs platelet‐rich plasma: Activity against chronic wound microbiota

Oliveira

Teixeira

et al. 2019

International Wound Journal

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The objective was to evaluate Staphylococcus aureus and Pseudomonas aeruginosa colonisation of wounds treated with recombinant epidermal growth factor (EGF) and platelet‐rich plasma (PRP); to analyse the susceptibility profiles of S. aureus and P. aeruginosa isolates from wounds treated with EGF and PRP; and to describe the presence of infection in EGF‐treated and PRP‐treated wounds. Experimental study was performed using clinical specimens collected with swabs. Patients were treated with PRP and EGF in the outpatient clinic of a university hospital. Forty‐three isolates were obtained from 31 patients, 41.9% (13/31) of whom had been treated with EGF and 58.0% (18/31) with PRP. Ten of the 43 isolates were identified as S. aureus, 60.0% (6/10) of which were isolated from PRP‐treated wounds. Among the 33 P. aeruginosa isolates, 66.6% (22/33) were isolated from PRP‐treated wounds. Regarding antimicrobial susceptibility, only one strain isolated from an EGF‐treated wound was identified as methicillin‐resistant S. aureus (MRSA). Among the P. aeruginosa isolates, one obtained from a patient treated with EGF was multidrug‐resistant. Patients treated with EGF had no infections during the follow‐up period, and there was a significant difference between the 1st and 12th week in wound infection improvement in patients treated with PRP (P = .0078).

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“…Em estudo realizado no mesmo cenário, Staphylococcus aureus foi detectado em 71,4% (25/35) das feridas em amostras coletadas por swab, 2 pacientes tinham Staphylococcus aureus resistente à meticilina (MRSA), entretanto, de acordo com os critérios clínicos, 8 (22,9%) pacientes apresentaram sinal de infecção em suas feridas. As feridas colonizadas por MRSA não mostraram uma forte correlação com a presença de um maior número de sinais clínicos de infecção 20 . A maioria das feridas é colonizada por bactérias, enquanto apenas algumas têm características de infecção de ferida clinicamente relevante 21 .…”

Section: Discussionunclassified

Validade De Construto E Confiabilidade Da Escala Leg Ulcer Measurement Tool Adaptada Para O Brasil

Silveira¹,

Oliveira²,

Souza³

et al. 2022

Texto contexto - enferm.

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Objetivo: validar o construto e a confiabilidade da versão em português da escala Leg Ulcer Measurement Tool. Método: trata-se de uma pesquisa metodológica. A coleta de dados foi realizada entre janeiro e julho de 2019. Foram incluídos 105 participantes para a validação de construto e consistência interna e, desses, 50 participantes para a estabilidade intraobservador. Para a análise de validade de construto, foi realizada a análise fatorial; para a consistência interna, foi calculado o coeficiente alfa de Cronbach e para a estabilidade, o coeficiente de correlação intraclasse. Resultados: foram confirmados 12 itens para a realidade brasileira, cumprindo todas as exigências teóricas do modelo da análise fatorial. Quanto à análise da consistência interna (confiabilidade), os valores do coeficiente de alfa de Cronbach (α=0,711) demonstraram que o conjunto de itens que compõem a escala mede as mesmas características e apresenta consistência interna. Na análise da estabilidade global (ICC=0,823), os escores da Leg Ulcer Measurement Tool no teste e reteste apresentaram boa concordância, demonstrando que a escalaadaptada é estável. Conclusão: A escala Leg Ulcer Measurement Tool adaptada para a língua portuguesa contém 12 itens, com pontuação de 0 a 4, e produz, assim, um escore que varia de 0 a 48 pontos. Apresenta validade de construto analisada pela análise fatorial e confiabilidade em termos de consistência interna e estabilidade. DESCRITORES:Pesquisa metodológica em enfermagem. Avaliação em enfermagem. Estudos de validação. Úlcera da perna. Úlcera varicosa. Pé diabético.

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Monitoring and Molecular Characterization of Staphylococcus aureus Isolated from Chronic Wounds

Abstract: Compared with those colonized by MSSA, chronic wounds colonized by MRSA did not display a strong correlation with the presence of a greater number of clinical signs of infection.

Cited by 10 publications

References 30 publications

Construct Validity and Reliability of the Leg Ulcer Measurement Tool Scale Adapted for Brazil

Construct Validity and Reliability of the Leg Ulcer Measurement Tool Scale Adapted for Brazil

Epidermal growth factor vs platelet‐rich plasma: Activity against chronic wound microbiota

Validade De Construto E Confiabilidade Da Escala Leg Ulcer Measurement Tool Adaptada Para O Brasil

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