Ciprofloxacin-Resistant Kerstersia gyiorum Isolated From a Chronic Wound in Brazil: A Case Report

Pires, Bruna Maiara Ferreira Barreto; Oliveira, Beatriz Guitton Renaud Baptista de; Oliveira, Fernanda Pessanha de; Silva, Keila de Cássia Ferreira de Almeida; Peloso, Pedro Fernandez Del; Ferreira-Carvalho, Bernadete Teixeira; Paula, Geraldo Renato de; Teixeira, Lenise Arneiro

doi:10.25270/wmp.2020.10.4245

Cited by 4 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resistance to norfloxacin (quinolone) was observed in only two isolates. In K. gyiorum human isolates, resistance to ciprofloxacin (quinolone) was reported in different countries, including Brazil ( Pence et al, 2013 ; Li et al, 2019 ; Pires et al, 2020 ). The main mechanism of fluoroquinolone resistance in Gram-negative bacteria is the accumulation of mutations in DNA gyrase and DNA topoisomerase IV ( Bush et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of twelve genomes of the bacterium Kerstersia gyiorum from brown-throated sloths (Bradypus variegatus), the first from a non-human host

Carhuaricra-Huaman,

Gonzalez,

Ramos

et al. 2024

PeerJ

View full text Add to dashboard Cite

Kerstersia gyiorum is a Gram-negative bacterium found in various animals, including humans, where it has been associated with various infections. Knowledge of the basic biology of K. gyiorum is essential to understand the evolutionary strategies of niche adaptation and how this organism contributes to infectious diseases; however, genomic data about K. gyiorum is very limited, especially from non-human hosts. In this work, we sequenced 12 K. gyiorum genomes isolated from healthy free-living brown-throated sloths (Bradypus variegatus) in the Parque Estadual das Fontes do Ipiranga (São Paulo, Brazil), and compared them with genomes from isolates of human origin, in order to gain insights into genomic diversity, phylogeny, and host specialization of this species. Phylogenetic analysis revealed that these K. gyiorum strains are structured according to host. Despite the fact that sloth isolates were sampled from a single geographic location, the intra-sloth K. gyiorum diversity was divided into three clusters, with differences of more than 1,000 single nucleotide polymorphisms between them, suggesting the circulation of various K. gyiorum lineages in sloths. Genes involved in mobilome and defense mechanisms against mobile genetic elements were the main source of gene content variation between isolates from different hosts. Sloth-specific K. gyiorum genome features include an IncN2 plasmid, a phage sequence, and a CRISPR-Cas system. The broad diversity of defense elements in K. gyiorum (14 systems) may prevent further mobile element flow and explain the low amount of mobile genetic elements in K. gyiorum genomes. Gene content variation may be important for the adaptation of K. gyiorum to different host niches. This study furthers our understanding of diversity, host adaptation, and evolution of K. gyiorum, by presenting and analyzing the first genomes of non-human isolates.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of twelve genomes of the bacterium Kerstersia gyiorum from brown-throated sloths (Bradypus variegatus), the first from a non-human host

Carhuaricra-Huaman,

Gonzalez,

Ramos

et al. 2024

PeerJ

View full text Add to dashboard Cite

show abstract

“… 15 Under the action of fluoroquinolones, bacteria develop drug resistance through chromosomal gene mutation or a plasmid-mediated mechanism, including target site change, decreased membrane permeability, and drug inactivation. 16 The mechanism of drug resistance in K. gyiorum may involve a gene-encoding efflux pump; 17 however, this requires further study.…”

Section: Discussionmentioning

confidence: 99%

“…Since K. gyiorum was named, it has been reported in various diseases, such as chronic ear infection, 2 chronic lower limb infection, 12 hemorrhagic dermatitis, 14 chronic venous insufficiency, 17 respiratory tract infection, 27 urinary tract infection, 28 thromboangiitis obliterans, 21 and chronic osteomyelitis 23 ( Table 2 ). K. gyiorum mainly causes chronic ear and leg infections, the pathogenic mechanism of which remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Kerstersia gyiorum isolated for the first time from two patients with neurodegenerative disease: report of two unusual cases and a review of the literature

et al. 2023

View full text Add to dashboard Cite

With the development of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S ribosomal RNA (rRNA) gene sequencing, increasing numbers of new microorganisms are being discovered. In this report, Kerstersia gyiorum was isolated for the first time from the sputum of two elderly patients with neurodegenerative disease, and integrated traditional Chinese and Western medicine was used for treatment. The bacteria’s growth characteristics, biochemical reaction characteristics, sensitivity to antibiotics, and the patients’ treatment are described, with a review of previous reports.

show abstract

“…In human K. gyiorum isolates, resistance to ciprofloxacin (quinolone) was reported in different countries including Brazil (Pence et al, 2013;Li et al, 2019b;Pires et al, 2020). The main mechanism of fluoroquinolone resistance in Gram-negative bacteria is the accumulation of mutations in DNA gyrase and DNA topoisomerase IV (Bush et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Análise genômica de isolados bacterianos de cobaias (Cavia porcellus) e preguiças-de-garganta-marrom (Bradypus variegatus)

Huaman

View full text Add to dashboard Cite

show abstract

Ciprofloxacin-Resistant Kerstersia gyiorum Isolated From a Chronic Wound in Brazil: A Case Report

Cited by 4 publications

References 0 publications

Analysis of twelve genomes of the bacterium Kerstersia gyiorum from brown-throated sloths (Bradypus variegatus), the first from a non-human host

Analysis of twelve genomes of the bacterium Kerstersia gyiorum from brown-throated sloths (Bradypus variegatus), the first from a non-human host

Kerstersia gyiorum isolated for the first time from two patients with neurodegenerative disease: report of two unusual cases and a review of the literature

Análise genômica de isolados bacterianos de cobaias (Cavia porcellus) e preguiças-de-garganta-marrom (Bradypus variegatus)

Contact Info

Product

Resources

About