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The objective of the present study is to conduct a non-destructive characterisation analysis of three fresco fragments from Iklaina, southern Peloponnese, Greece, in order to identify their manufacture techniques. Furthermore, this study aims at using the results of the scientific and analytical analyses to produce accurate replicas of these fragments (use of similar composition mortars, pigments, and manufacture techniques), which can be used for the restoration program of the site. These replicas will be exhibited alongside the originals in the new Pylos Archaeological Museum.Due to the high archaeological value of the objects, the analytical approach we followed was completely non-destructive and was based on the parallel use of optical microscopy, p-XRF and SEM/EDS. The analyses suggest the use of Egyptian blue pigment for the backgrounds. The wavy black coil of hair was painted with an organic black pigment. The use of inorganic pigments has been revealed on the upper layers on the basis of the identification of minerals and rocks. The substrate of the wall paintings is made of aluminosilicate lime mortars. The results corroborate the suggestions of previous studies for the pigments comprising the Mycenaean artistic palette and provides further insight on the artistic and technological choices made by Mycenaean artists.