Niemann-Pick type C (NPC) disease is an autosomal recessive lysosomal storage disease that is caused by a mutation of the NPC1 or NPC2 gene, in which un-esterified cholesterol and sphingolipids accumulate mainly in the liver, spleen, and brain. Abnormal lysosomal storage leads to cell damage, neurological problems, and premature death. The time of onset and severity of symptoms of NPC disease are highly variable. The molecular mechanisms that are responsible for NPC disease pathology are far from being understood. The present study generated and characterized a zebrafish mutant that lacks Npc2 protein that may be useful for studies at the organismal, cellular, and molecular levels and both small-scale and high-throughput screens. Using CRISPR/Cas9 technology, we knocked out the zebrafish homolog of NPC2. Five-day-old npc2 mutants were morphologically indistinguishable from wildtype larvae. We found that live npc2–/– larvae exhibited stronger Nile blue staining. The npc2–/– larvae exhibited low mobility and a high anxiety-related response. These behavioral changes correlated with downregulation of the mcu (mitochondrial calcium uniporter) gene, ppp3ca (calcineurin) gene, and genes that are involved in myelination (mbp and mpz). Histological analysis of adult npc2–/– zebrafish revealed that pathological changes in the nervous system, kidney, liver, and pancreas correlated with inflammatory responses (i.e., the upregulation of il1, nfκβ, and mpeg; i.e., hallmarks of NPC disease). These findings suggest that the npc2 mutant zebrafish may be a model of NPC disease.

No abstract

No abstract

A common global trend in modern aquaculture is to reduce or replace fishmeal in fish diets with alternative protein sources, mainly from protein‐rich plants such as soybean; however, plant‐based aquafeeds are a source of substantial amounts of phytoestrogens. Phytoestrogens belong to endocrine‐disrupting chemicals (EDCs) which can negatively interfere with fish reproduction; thus, they should be avoided in sturgeon diet. Therefore, the aim of the study was to evaluate concentrations and the profile of dietary isoflavone phytoestrogens in the two commercial sturgeon diets. A substantial amount of isoflavones, reaching in total from 1.5 to 50 mg/kg of feed, were found in analysed diets. Genistein and daidzein, which are typical constituents of soybean, were the most abundant phytoestrogens in the analysed feeds. Equol, the third most abundant phytoestrogen in the tested samples, was originated most probably from animal components. Additionally, the accumulation of dietary isoflavones in the liver, blood and gonads of two commonly farmed sturgeon species (Acipenser gueldenstaedtii and Acipenser baerii) after long‐term dietary exposure was also assessed. Significant concentrations of isoflavones were found in liver and blood samples of both sturgeon species at 600th and 800th dph, indicating high accumulation of these compounds, which may lead to endocrine disturbance.

Optimal development of the gut is important for nutrient absorption and for poultry to resist diseases. The aim of the study was to compare gastrointestinal tract morphometrics, small intestine microstructure, as well as the amount of pasture matter and feed ingested by the birds with outdoor access presenting either an outdoor-preferring, moderate-outdoor or indoor-preferring ranging profile. Sixty non-beak trimmed birds per strain: broiler hybrid Sasso and Polish indigenous green-legged partridge were housed from week 5 to 10 in groups of 10, under conditions of EU organic meat chicken production. Pens with outdoor ranges were video recorded, to obtain frequencies of the birds’ range use. Statistical analysis was conducted applying generalized linear mixed models, applying the ranging profile as a fixed effect and pen as a random factor. The weight of the pasture matter in bird crops was the highest in moderate-outdoor profiled green-legged partridges, as compared to other ranging profiles (p = 0.04). In Sasso, villi in the small intestines were significantly higher in the outdoor-preferring compared to indoor-preferring profiled birds (p = 0.04), while their area was larger in the outdoor-preferring Sasso birds (p = 0.01). The level of development of the gastrointestinal tract and its content may be a potential indicator of the birds’ ranging profile and forage consumption.

Accessory respiratory organs (AROs) are a group of anatomical structures found in fish, which support the gills and skin in the process of oxygen uptake. AROs are found in many fish taxa and differ significantly, but in the suborder Anabantoidei, which has a labyrinth organ (LO), and the family Clariidae, which has a dendritic organ (DO), these structures are found in the suprabranchial cavity (SBC). In this study, the SBC walls, AROs, and gills were studied in anabantoid (Betta splendens, Ctenopoma acutirostre, Helostoma temminckii) and clariid (Clarias angolensis, Clarias batrachus) fishes. The histological structure of the investigated organs was partially similar, especially in relation to their connective tissue core; however, there were noticeable differences in the epithelial layer. There were no significant species-specific differences in the structure of the AROs within the two taxa, but the SBC walls had diversified structures, depending on the observed location. The observed differences between species suggest that the remarkable physiological and morphological plasticity of the five investigated species can be associated with structural variety within their AROs. Furthermore, based on the observed histology of the SBC walls, it is reasonable to conclude that this structure participates in the process of gas exchange, not only in clariid fish but also in anabantoids.

Background: The aim of the study has been to compare the effect of dietary supplementation of β-1,3/1,6-glucan, Lactobacillus plantarum bacteria or their mixture on the growth performance, selected parameters of the immune system as well as the liver and intestinal histology of roach. Fish were fed for 14 days with four different diets, each treatment being carried out in triplicate. In control group, fish were fed dry commercial starter feed Aller Performa 2 (Aller Aqua, Denmark). The other experimental fish groups received the same commercial starter feed supplemented with: 1% β-1,3/1,6-glucan (Leiber® BetaS) in group G; 10 8 cfu L. plantarum g − 1 in group L; 1% β-1,3/1,6-glucan + 10 8 cfu L. plantarum g − 1 in group G + L. The stimulating effect of the tested preparations was evaluated once the feeding with commercial feed exclusively was resumed and 2 weeks afterwards. Results: No effect on the survivability and growth performance of the fish was observed in any of the groups. Supplementation of feed with β-1,3/1,6-glucan improved (P < 0.05) selected parameters of innate humoral immunity and the pinocytotic activity of phagocytes. Increased respiratory burst activity of head kidney phagocytes (RBA) was observed in groups L and G + L (P < 0.05), and the effect persisted for 2 weeks after the commercial feed regime was resumed. An analogous tendency was determined for the killing activity of phagocytes (PKA) of the head kidney with respect to Aeromonas hydrophila, although this effect appeared only during the feed supplementation period. Supplying roach with β-1,3/1,6-glucan, singly or with L. plantarum, had no effect (P > 0.05) on the proliferation of mitogen-activated lymphocytes. However, an increase in the number of CD3-positive cells and goblet cells was noticed in the digestive system of the L group fish (P < 0.05).

No abstract