Sex-related gene expression profiles in various tissues of juvenile Russian sturgeon (Acipenser gueldenstaedtii)

Fajkowska, Magdalena; Głowacka, Dominika Kinga; Adamek‐Urbańska, Dobrochna; Ostaszewska, Teresa; Krajnik, Katarzyna Alicja; Rzepkowska, Małgorzata

doi:10.1016/j.aquaculture.2018.10.066

Cited by 12 publications

(9 citation statements)

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“…A total of 60 genes (involving 755 unigenes), reported as active in the gonad development of sturgeon [10,[42][43][44], were detected to be present in the full-length gonad transcriptome of 3-year-old A. schrenckii. Therefore, the 60 early gametogenesis related genes and their NR annotations of full-length unigenes from the Iso-Seq of A. scipenserkii gonads are listed in Table 3 and Supplementary Table 6, respectively.…”

Section: Search For Genes Involved In Early Gametogenesis Of a Schrementioning

confidence: 99%

Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii)

Zhang¹,

Zhou²,

Li³

et al. 2020

Front Zool

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Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii) AbstractBackground: Sturgeons (Acipenseriformes) are polyploid chondrostean fish that constitute an important model species for studying development and evolution in vertebrates. To better understand the mechanisms of reproduction regulation in sturgeon, this study combined PacBio isoform sequencing (Iso-Seq) with Illumina short-read RNA-seq methods to discover full-length genes involved in early gametogenesis of the Amur sturgeon, Acipenser schrenckii.Results: A total of 50.04 G subread bases were generated from two SMRT cells, and herein 164,618 nonredundant full-length transcripts (unigenes) were produced with an average length of 2782 bp from gonad tissues (three testes and four ovaries) from seven 3-year-old A. schrenckii individuals. The number of ovary-specific expressed unigenes was greater than those of testis (19,716 vs. 3028), and completely different KEGG pathways were significantly enriched between the ovary-biased and testis-biased DEUs. Importantly, 60 early gametogenesisrelated genes (involving 755 unigenes) were successfully identified, and exactly 50% (30/60) genes of those showed significantly differential expression in testes and ovaries. Among these, the Amh and Gsdf with testisbiased expression, and the Foxl2 and Cyp19a with ovary-biased expression strongly suggested the important regulatory roles in spermatogenesis and oogenesis of A. schrenckii, respectively. We also found the four novel Sox9 transcript variants, which increase the numbers of regulatory genes and imply function complexity in early gametogenesis. Finally, a total of 236,672 AS events (involving 36,522 unigenes) were detected, and 10,556 putative long noncoding RNAs (lncRNAs) and 4339 predicted transcript factors (TFs) were also respectively identified, which were all significantly associated with the early gametogenesis of A. schrenckii.

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Section: Search For Genes Involved In Early Gametogenesis Of a Schrementioning

confidence: 99%

Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii)

Zhang¹,

Zhou²,

Li³

et al. 2020

Front Zool

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“…Parentage analysis gives accurate information about effective population size, inbreeding assessment, genetic diversity level and migration pattern, which are essential for effective conservation and restocking programme of fish in their wild habitat (Liu et al 2012;Schreier et al 2013;Roques et al 2016). Gene expression analysis is helpful in sex determination, identification of specific candidate genes (Chen et al 2018;Degani et al 2019;Fajkowska et al 2019), developmental biology (Abdolahnejad et al 2015), discovery of biomarker and ecotoxicological-related studies of sturgeon (Silvestre et al 2010). Germ cell transplantation has immense application in artificial breeding and seed production, conservation of endangered species, gene banking, primordial germ cell cryopreservation and surrogate reproduction through germline chimera (Kazama-Wakabayashi et al 1999;Yoshizaki et al 2003;P seni cka et al 2015).…”

Section: Application Of Above Techniques In Aquaculture and Conservationmentioning

confidence: 99%

“…The brain of sturgeon is the prime organ that stimulates other organs and tissues to differentiate into male or female; double sex and mab‐3‐related transcription factor 1 ( dmrt1 ) and foxl2 were key genes that played pivotal roles in sex differentiation in Russian sturgeon ( A. gueldenstaedtii ) (Fajkowska et al . 2019). The expression of cytochrome P450 family 17 subfamily a polypeptide 1 ( cyp17a1 ), androgen receptor ( ar ), dmrt1 and Sry box‐containing gene 9 ( sox9 ) genes were significantly higher in testis than in ovary of stellate sturgeon ( A. stellatus ) (Burcea et al .…”

Section: Gene Expression Analysismentioning

confidence: 99%

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Trends in aquaculture and conservation of sturgeons: a review of molecular and cytogenetic tools

Chandra

Fopp‐Bayat

2020

Reviews in Aquaculture

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Sturgeons are the most primitive, endangered groups of vertebrates on the planet and often referred to as 'living fossils'. Their natural populations are decreasing due to habitat destruction, blockage of spawning migration, pollution and overexploitation for their expensive black caviar and boneless meat. Currently, existing populations have little chance to survive without artificial breeding and sustainable ranching programmes. Convention on International Trade in Endangered Species (CITES) is regulating international trade of all Acipenseriformes and their products for effective conservation. The aim of this review paper was to describe the main innovative applications in sturgeon aquaculture and conservation, such as genome engineering techniques applied in sturgeon's reproduction, molecular and ploidy analyses of embryo and larvae, sex determination, genome sequencing, gene expression analysis and genome transplantations. Further research is required to improve cryopreservation techniques, embryo banking and quick identification of disease in sturgeon aquaculture and conservation programme.

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“…A total of 60 genes (involving 755 unigenes), reported as active in the gonad development of sturgeon [10,[42][43][44], were detected to be present in the full-length gonad transcriptome of 3-year-old A. schrenckii. Therefore, the 60 early gametogenesis related genes and their NR annotations of fulllength unigenes from the Iso-Seq of A. scipenserkii gonads are listed in Table 3 and Supplementary Table 6, respectively.…”

Section: Search For Genes Involved In Early Gametogenesis Of a Schrementioning

confidence: 99%

Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii)

Zhang¹,

Zhou²,

Li³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Sturgeons (Acipenseriformes) are polyploid chondrostean fish that constitute an important model species for studying development and evolution in vertebrates. To better understand the mechanisms of reproduction regulation in sturgeon, this study combined PacBio isoform sequencing (Iso-Seq) with Illumina short-read RNA-seq methods to discover full-length genes involved in early gametogenesis of the Amur sturgeon, Acipenser schrenckii . Results: A total of 50.04 G subread bases were generated from two SMRT cells, and herein 164,618 nonredundant full-length transcripts (unigenes) were produced with an average length of 2,782 bp from gonad tissues (three testes and four ovaries) from seven 3-year-old A. schrenckii individuals. The number of ovary-specific expressed unigenes was greater than those of testis (19,716 vs. 3,028), and completely different KEGG pathways were significantly enriched between the ovary-biased and testis-biased DEUs. Importantly, 60 early gametogenesis-related genes (involving 755 unigenes) were successfully identified, and exactly 50 percent (30/60) genes of those showed significantly differential expression in testes and ovaries. Among these, the Amh and Gsdf with testis-biased expression, and the Foxl2 and Cyp19a with ovary-biased expression strongly suggested the important regulatory roles in spermatogenesis and oogenesis of A. schrenckii , respectively. We also found the four novel Sox9 transcript variants, which increase the numbers of regulatory genes and imply function complexity in early gametogenesis. Finally, a total of 236,672 AS events (involving 36,522 unigenes) were detected, and 10,556 putative long noncoding RNAs (lncRNAs) and 4,339 predicted transcript factors (TFs) were also respectively identified, which were all significantly associated with the early gametogenesis of A. schrenckii . Conclusions: Overall, our results provide new genetic resources of full-length transcription data and information as a genomic-level reference for sturgeon. Crucially, we explored the comprehensive genetic characteristics that differ between the testes and ovaries of A. schrenckii in the early gametogenesis stage, which could provide candidate genes and theoretical basis for further the mechanisms of reproduction regulation of sturgeon.

show abstract

Sex-related gene expression profiles in various tissues of juvenile Russian sturgeon (Acipenser gueldenstaedtii)

Cited by 12 publications

References 80 publications

Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii)

Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii)

Trends in aquaculture and conservation of sturgeons: a review of molecular and cytogenetic tools

Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Acipenser schrenckii)

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